Receptor-ligand interactions, affinity capillary electrophoresis

Affinity capillary electrophoresis is an electrophoretic mode that takes advantage of the specific interactions of receptors, antibodies, or ligands with the analyte. In contrast to other CE modes, ACE is not dedicated to general analysis, but rather is focused on measuring molecular interactions of the solute with specific receptors. 

E Mito, Y Zhang, S Esquivel, FA Gomez. Estimation of receptor-ligand interactions by the use of a two-marker system in affinity capillary electrophoresis. Anal Biochem 280 209-215, 2000. 

Affinity Capillary Electrophoresis-Mass Spectrometry. Affinity capillary electrophoresis was originally used for the determination of the binding constants of small molecules to proteins (47-49). This solution-based technique is rapid and requires only small amounts of ligands. Affinity constants are measured based on the mobility change of the ligand on interaction with the receptor present in the electrophoretic buffer (50). By combining affinity capillary electrophoresis with on-line mass spectrometric detection and... 

Affinity capillary electrophoresis (ACE) is used to characterize receptor-ligand interactions. The technique depends upon a shift in the electrophoretic mobility of the receptor upon complexation with a charged ligand. The... 

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