Buffers acetamide

Acetamide has been used experimentally as a source of nonprotein nitrogen for sheep and dairy cattie (13). It does not appear to be toxic in amounts of about 2—3% of ration. Buffering the diet with dibasic acids serves to allow higher levels of intake because the ammonia Hberated in the digestive process is then scavenged. 

P 20] Via two micro syringes, a mixture of 0.32 M p-nitrophenyl-/lD-galacto-pyranoside and y0-galactosidase and 3.2 mM p-nitrophenyl-2-acetamide-2-deoxy-/3-D-glucopyranoside in 50% phosphate buffer (pH 8)-acetonitrile and /Igalactosi-dase (20 U) in 10 ml of the same solvent system were fed into the micro channel [26]. Identical flow rates were used and the reaction was carried out for 0-30 min at 37 °C. 

Relationship between rat intestinal absorption clearance and lipid solubility. The results shown with the squares represent the relationship between intestinal absorption clearance (ka) observed from the in situ jejunum loop in the presence ( ) and absence ( ) of cyclosporin A in rats and octanol-buffer (pH 7.0) partition coefficients (log D) determined in this study. The numbers refer to 1, atenolol 2, nadolol 3, acetamide 4, celiprolol 5, acebutolol 6, doxorubicin 7, timolol 8, sulfathiazole 9, quinidine 10, sulfamethoxazole 11, digoxin 12, cyclosporin A 13, vinblastine 14, b-estradiol 15, verapamil. Modified from A.Tsuji and I. Tamai. Pham. Res., 13 963—977 (1996). 

B SA, 7V,0-bis-(trimethylsilyl)-acetamide BMS, bis(2-mercaptoethyl)snlfone Buchner fuimel Buffers, aqueous... 

Examples of chiral CE separations of racemic drugs are the following. (/ )-(-)-ketoprofen has successfully been separated from ketoprofen and detected (Fig. 4).f (5)-(+)-ketoprofen is the active component. Also, simultaneous chiral separation of a basic drug compound, 2(/ )-A-[l-(6-amino-pyridin-2-ylmethyl) pip-eridin-4-yl]-2-[(l/ )-3,3-difluorocyclopentyl]-2-hydroxy-2-phenyl-acetamide, and its chiral acidic intermediate, (/ ,/ ) l-(2,2-difluorocyclopentyl)-phenylacetic acid, has been achieved by CE using a single-isomer CD, octakis (2,3-diacetyl-6-sulfo)-y-CD (ODAS-y-CD).P l Carnitine has been separated using 50 mM DM-p-CD in 20 mM phosphate buffer (pH 4.3) as chiral selector. The separations are done at 30° C in a fused-silica capillary, dynamically coated with triethanolamine present in the background electrolytes. 

Kupferberg (38) reported that primidone formed a trlmethylsilyl derivative with N,0-bis-(trimethylsilyl)-acetamide in pyridine. Primidone was extracted from plasma buffered to pH 7.2, using chloroform. After several solvent partitioning steps, the primidone was treated with the reagent for 20 minutes. 

Sodium acetate anhydrous Sodium diacetate buffer, electrodeless plating Sodium glycolate buffer, electroplating baths Sodium phosphate buffer, emulsions Sodium citrate buffer, explosives Acetamide buffer, eye drops Boric acid... 

The reaction kinetics of acetamide and benzamide o-(phenoxycarbonyl)oximes have been studied in aqueous buffers over a wide pH range, with cyclization dominant at high pH, and hydrolysis at lower values. A Hammett plot for the benzamide series suggests that A-deprotonation occurs, followed by a rate-limiting step involving concerted N-C bond formation and C-0 bond breaking. 

Hydrolysis ofp-nitrophenyl-(3-D-galactopyranoside was performed in buffered media, whereas transgalactosylation of p-nitrophenyl-2-acetamide-2-deoxy-beta-D-glucopyranoside was conducted in buffer-acetonitrUe solvent system. The authors did not use immobilized enzyme. The reaction was performed by separate loading of the enzyme in phosphate buffer solution and the substrate solution in... 

Solution-phase continuous flow technique hydrolysis of p-nitrophenyl- 3-D-galacto-pyranoside on buffered media transgalactosylation on p-nitrophenyl-2-acetamide-2-deoxy-beta-D-glucopyranosidein buffer-acetonitrile solvent system both reactions enhanced compared to batch system no isomers isolated... 

Frankenberger and Tabatabai have described an assay for soil amida.ses, which catalyse the hydrolysis of aliphatic amides to carboxylic acids and ammonia. Their assay requires toluene-treated soils to be incubated at 37°C with 0.05M substrate (formamide, acetamide, propionamide) for either 2 or 24h. The suspensions were buffered at pH 8.5 the choice of buffer influenced the amounts of NH4 released, which formed the basis for estimating activities. The pH optimum for hydrolysis of all substrates was about pH 8.5 the optimum temperature was about 65°C. Reaction rates in the presence of toluene were linear both with time and with the amounts of soil when substrate concentrations were 0.05M. At this concentration, and above, the reactions exhibited zero-order kinetics for the recommended assay periods. 

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