Tris aminomethane buffer

These same dependencies will, in general, apply to the heat of ionization of the buffer acid, AH. Thermodynamic quantities, namely, AH°, have been reported for some buffer substances, and it is found that A//° is temperature dependent. Bates and Hetzer studied the temperature dependence of for the important buffer tris(hydroxymethyl)aminomethane (TRIS), finding... 

Addex-tham AI3-03948 Aminotrimethylolmethane Aminotris(hydroxymethyl)methane Apiroserum Tham Caswell No. 036 EINECS 201-064-4 EPA Pesticide Chemical Code 083901 HSDB 3408 Methanamine, 1,1,1-tris(hydroxymethyl)- NSC 6365 Pehanorm Talatrol Tham THAM THAM-E Trimethylolamino-methane Tris Tris (buffering agent) Tris-base Tris buffer Tris, free base Tris-hydroxymethyl-aminomethan Tris-... 

Phos, phosphate buffer Tris, tris(hydroxymethyl)aminomethane ammo, ammonium sulfate lact, D-lactate glyc, glycerol trit, Triton X-100 gaba, -y-aminobutyric acid. 

Activity was not released by 1.0% Reten 205M (a cationic polymer) (Hercules Inc., Wilmington, Del.), 0.13-0.38M 2-mercapto-ethanol, liquid phenol, incubation with 0.1% lysozyme, osmotic shock (I), or the following surfactants 0.4% Triton X-100 (Rohm and Haas Co., Philadelphia, Pa.), 0.4% sodium lauryl sulfate, 0.5% digitonin in 0.05M tris buffer (trishydroxymethyl-aminomethane). 

Radioiodide has also been separated from iodine-labeled proteins (human serum albumin and Bovine IgG) by thin-layer gel filtration on Sephadex G-200 and G-75 superfine layers (163). (Sephadex is a modified dextran.) The protein solutions were applied in amounts of 5-10 vJL to the Sephadex layers and the angle of slope of the layers was 15°. Elution was carried out with 0.2 M Tris buffer (Tris = tris (hydroxymethyl) aminomethane) at pH 8.0. The buffer contained Tween 80. Separations took 45-60 min on Sephadex G-75 and 3-4 h on Sephadex G-200 and were particularly good on the latter. 

Precaution Wear eye and face protection, protective gloves and clothing avoid elevated temps. reacts with with copper, aluminum, zinc and their alloys Uses De-emulsifier Trade Names Ethoduomeen OV/13 Tris (hydroxymethyl) aminomethane CAS 77-86-1 25149-07-9 108195-864 EINECS/ELINCS 201-0644 Synonyms 2-Amino-2-(hydroxymethyl)-1,3-propanediol Aminotrimethylol-methane Aminotris(h roxymethyl) methane THAM Tris Trisamine Tris Buffer Tris (hydroxymethyl) methanamine Tris (hydroxymethyl) methylamine 1,1,1-Tris (hydroxymethyl) methylamine Trometamol Tromethamine (INCI)... 

AACC Method32-07for TDF, SDF, and IDF. This approved method is a modification of the Prosky method. The phosphate buffer is replaced by a buffer of 2(A[-morpholino)ethanesulfonic acid (MES) [4432-31-9] and tris(hydroxymethyl)aminomethane (TRIS) [77-86-1]. Precision is improved and analysis time is reduced. I DE may be determined on a separate sample or calculated by summing the SDE and IDE values. The MES /TRIS-modified method has received final approval by AACC and official first action by AO AC (5). 

Heavy metal contamination of pH buffers can be removed by passage of the solutions through a Chelex X-100 column. For example when a solution of 0.02M HEPES [4-(2-HydroxyEthyl)Piperazine-l-Ethanesulfonic acid] containing 0.2M KCl (IL, pH 7.5) alone or with calmodulin, is passed through a column of Chelex X-100 (60g) in the K" " form, the level of Ca ions falls to less than 2 x 10" M as shown by atomic absorption spectroscopy. Such solutions should be stored in polyethylene containers that have been washed with boiling deionised water (5min) and rinsed several times with deionised water. TES [, N,N, -Tetraethylsulfamide] and TRIS [Tris-(hydroxymethyl)aminomethane] have been similarly decontaminated from metal ions. 

Chloroform, sodium chloride, anhydrous sodium sulfate, sulfuric acid (97%), hydrochloric acid (36%), sodium bicarbonate, trifluoroacetic acid, tris(hydro-xymethyl)aminomethane (Tris), special grade Water, high-performance liquid chromatography grade 0.1 M Phosphate buffer solution (pH 7.0)... 

Note that, just like for the first-order expression in Equation 5.12 also the second-order expression in Equation 5.18 applies to field-swept spectra, and a different expression found in EPR textbooks (Pake and Estle 1973) applies to frequency-swept spectra. The effect of including a second-order contribution to the central hyperfine splitting is illustrated in Figure 5.7 on the spectrum of a not uncommon contaminant of metalloprotein preparations Cu(II) ion coordinated by nitrogens of tris-hydroxy-ethyl aminomethane or Tris buffer. 

FIGURE 5.7 Second-order hyperfine shift in the X-band EPR of the Cu(II)-Tris complex. The thin solid line is the experimental spectrum of 1.5 mM CuS04 in 200 mM Tris-HCl buffer, pH 8.0 taken at v = 9420 MHz and T = 61 K. Tris is tris-(hydroxymethyl)aminomethane or 2-amino-2-hydroxymethyl-l,3-propanediol. The broken lines are simulations using the parameters g = 2.047, gN = 2.228, Atl = 185 gauss. In the lower trace the second-order correction has been omitted. 

An ethanolic solution of 3H-Flunisolide is diluted with tris-(hydroxymethyl)-aminomethane/hydro-chloric acid buffer and 0.1% gelatin such that 0.1 ml portion contains 8,000 to 10,000 cmp activity,... 

A review by Galli et al. describes several buffer-absorbing chromophores as co-ions. These include phthalate, PDG (2,6-pyridinedicarboxylic acid), PMA (1,2,4,5-benzenetetra-carboxylic acid or pyromellitic acid), TMA (trimellitic acid), MES, 2,4-dihydrobenzoic acid with s-aminocaproic acid, p-hydroxybenzoate, p-anisate, 3,5-dinitrobenzoic acid, salicylic acid with TRIS, benzoic acid with tris (hydroxymethyl)aminomethane (TRIS), and many others. On the other hand, some inorganic chromophores such as chromate (Figure 9) or molybdate may be added to a buffer. A BGE-containing chromate should have a pH above 8, because it precipitates below this value. The advantage of a TRIS buffer or buffers at around pH 6 is that carbonate will not interfere with the separation because it is not soluble in TRIS or at lower pHs. 

Measurements made at pH 9.0 in 0.02 M tris(hydroxymethyl)aminomethane buffer, 25°C. Stock solutions of substrate were made in acetonitrile hence, the final buffer also contained 6.7% acetonitrile. 

Reagents for Enzyme-Immunoassay. Tris buffer contained 0.05 M Tris (hydroxymethyl) aminomethane, pH 7.5 0.05, with 0.1% human serum albumin and 0.01% sodium azide. The H O -methanol fixative contained 0.3% in absolute methanol prepared just before use... 

Huisman, T. H. J., and Dozy, A. M. (1965). Studies on the heterogeneity of hemoglobin. IX. The use of Tris(hydroxylmethyl)aminomethane-HCl buffers in the anion-exchange chromatography of hemoglobins. J. Chromatogr. 19, 160-169. 

If an amine P-NH2 is used in the aqueous solution, one obtains RCONHP instead of RCOOH. Rates of cleavage of three acyl nitrophenyl esters were followed by the appearance of p-nitrophenolate ion as reflected by increased absorbances at 400 nm. The reaction was carried out at pH 9.0, in 0.02 M tris(hydroxymethyl)aminomethane buffer, at 25°C. Rate constants were determined from measurements under pseudo-first-order conditions, with the residue molarity of primary amine present in approximately tenfold excess. First-order rate graphs were linear for at least 80% of the reaction. With nitrophenyl acetate and nitrophenyl caproate, the initial ester concentration was 6.66xlO 5M. With nitrophenyl laur-ate at this concentration, aminolysis by polymer was too fast to follow and, therefore, both substrate and amine were diluted tenfold for rate measurements. 

Fig. 3. (a) Reaction of pyridoxal 5 -phosphate (PLP) with an amino-terminal amino group of hemoglobin (Hb). The reagent is in the form of a Schiffs base with tris(hydroxymethyl)aminomethane [77-86-1] (Tris) buffer, and the reaction is a transamination, (b) The resulting unstable Scbiff s base is reduced with... 

For illustration, we choose a widely used buffer called tris, which is short for tris(hydroxy-methyl)aminomethane. 

Potassium phosphate buffer solution. Similar l , data were obtained also for SYL-boundb Pr in tris(hydroxymethyl)aminomethane buffer solution. 

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