Aminomethane

H0CH3)3CNHH3 121.137 Tris(hydroxymethyl)aminomethane is available commercially as a primary standard. Dry at 100-103°C (<110°C). In titrations with a strong acid the equivalence point is at about pH 4.5-5. Equivalent weight is the formula weight. [J. H. Eossum, P. C. Markunas, and J. A. Riddick, Anal. Chem., 23 491 (1951).]... 

The end point for this titration is improved by titrating to the second equivalence point, boiling the solution to expel CO2, and retitrating to the second equivalence point. In this case the reaction is Na2C03 + 2H3O+ -> CO2 + 2Na+ + 3H2O TRIS stands for tr/s-(hydroxymethyl)aminomethane. 

AB, AEFD and TRIS AMINO are registered trademarks of ANGUS Chemical Company. Common name is tris(hydroxymethyl)aminomethane. 

These compounds are highly soluble in water. AMP, AMPD, AEPD, and DMAMP are completely miscible in water at 20 °C the solubihty of AB is 250 g/100 mL H2O at 20°C. They are generally very soluble in alcohols, slightly soluble in aromatic hydrocarbons, and nearly insoluble in aliphatic hydrocarbons tris(hydroxymethy1)aminomethane [77-86-1] is appreciably soluble only in water (80 g/100 mL at 20°C) and methanol. 

Fig. 3. (a) Reaction of pytidoxal 5 -phosphate (PLP) witii an amino-temiinal amino group of hemoglobin (Hb). The reagent is in the form of a Schiff s base with tris(hydroxymethyl)aminomethane [77-86-1] (Tris) buffet, and the reaction is a transamination, (b) The resulting unstable Schiff s base is reduced with... 

AACC Method32-07for TDF, SDF, and IDF. This approved method is a modification of the Prosky method. The phosphate buffer is replaced by a buffer of 2(A[-morpholino)ethanesulfonic acid (MES) [4432-31-9] and tris(hydroxymethyl)aminomethane (TRIS) [77-86-1]. Precision is improved and analysis time is reduced. I DE may be determined on a separate sample or calculated by summing the SDE and IDE values. The MES /TRIS-modified method has received final approval by AACC and official first action by AO AC (5). 

Heavy metal contamination of pH buffers can be removed by passage of the solutions through a Chelex X-100 column. For example when a solution of 0.02M HEPES [4-(2-HydroxyEthyl)Piperazine-l-Ethanesulfonic acid] containing 0.2M KCl (IL, pH 7.5) alone or with calmodulin, is passed through a column of Chelex X-100 (60g) in the K" " form, the level of Ca ions falls to less than 2 x 10" M as shown by atomic absorption spectroscopy. Such solutions should be stored in polyethylene containers that have been washed with boiling deionised water (5min) and rinsed several times with deionised water. TES [, N,N, -Tetraethylsulfamide] and TRIS [Tris-(hydroxymethyl)aminomethane] have been similarly decontaminated from metal ions. 

Chemical Designations - Synonyms Aminomethane Mercurialin Monomethylamine, anhydrous Chemical Formula CHjNHj... 

These same dependencies will, in general, apply to the heat of ionization of the buffer acid, AH. Thermodynamic quantities, namely, AH°, have been reported for some buffer substances, and it is found that A//° is temperature dependent. Bates and Hetzer studied the temperature dependence of for the important buffer tris(hydroxymethyl)aminomethane (TRIS), finding... 

Methylamine. This article supplements the one in Vol 1, A225—7 under Aminomethane and Derivatives CA Registry No 74-89-5 Uses in Explosives. Gaseous methylamine is passed into solid Amm Perchlorate below -6.3° to give useful monoproplnts. A mixt so prepd of 85% Amm Perchlorate and 15% methylamine has an Isp of 230 lbsec/lb at 20atm (Ref 1). 

FIGURE 3 Effect of the amount of cholesterol on the particle size. Phosphatidylcholine/cholesterol liposomes were prepared by the octyl glucoside dilution technique. The begin concentration of the mixed micelles was 150 mM octyl glucoside and 10 mM phosphatidylcholine in 10 mM tris(hydroxymethyl)aminomethane and 0.9% NaCl, pH 7.4. Dilution was performed with an automatic titration unit at a dilution rate (= dilution factor, relative to the initial volume, per unit of time) of 0.026 sec"l ( a and ) or 0.69 sec l ( and o). Mean diameters after dilution and ) and after filtration ( L and q) are repi sented. (Adapted from Jiskoot et al, 1986a.)... 

Tris-hydroxymethyl-aminomethane (THAM) has been evaluated in ischemic stroke to reduce mass effect and ICP. It acts as a bulfer, neutralizing acidosis on a local level, including in the brain parenchyma. It has been studied in animal models of stroke, showing an effect in reducing the size of and swelling from cerebral infarction. To date, however, THAM has not been studied in a controlled fashion in humans with ischemic stroke. 

Chloroform, sodium chloride, anhydrous sodium sulfate, sulfuric acid (97%), hydrochloric acid (36%), sodium bicarbonate, trifluoroacetic acid, tris(hydro-xymethyl)aminomethane (Tris), special grade Water, high-performance liquid chromatography grade 0.1 M Phosphate buffer solution (pH 7.0)... 

The products, perfluoro-l-aminomethylguanidine and periluoro-A-am inomethy 1 tri-aminomethane, and by-products of the reaction of fluorine with cyanoguanidine are extremely explosive in gas, liquid and solid states. 

Note that, just like for the first-order expression in Equation 5.12 also the second-order expression in Equation 5.18 applies to field-swept spectra, and a different expression found in EPR textbooks (Pake and Estle 1973) applies to frequency-swept spectra. The effect of including a second-order contribution to the central hyperfine splitting is illustrated in Figure 5.7 on the spectrum of a not uncommon contaminant of metalloprotein preparations Cu(II) ion coordinated by nitrogens of tris-hydroxy-ethyl aminomethane or Tris buffer. 

FIGURE 5.7 Second-order hyperfine shift in the X-band EPR of the Cu(II)-Tris complex. The thin solid line is the experimental spectrum of 1.5 mM CuS04 in 200 mM Tris-HCl buffer, pH 8.0 taken at v = 9420 MHz and T = 61 K. Tris is tris-(hydroxymethyl)aminomethane or 2-amino-2-hydroxymethyl-l,3-propanediol. The broken lines are simulations using the parameters g = 2.047, gN = 2.228, Atl = 185 gauss. In the lower trace the second-order correction has been omitted. 

TEP TL TLC TMB TMP TMP TMPG TNS TPB TRIS TRITC TTAB UA USDA-FSIS Triethylphosphine Total luminescence Thin layer chromatography T etramethylbenzidine 2,4,6,8-Tetrathiomorpholinopyrimido [5,4-d] pyrimidine Trimethylphosphine S S -Trimethoxyphenylglyoxal Potassium 2-p-toluidinylnaphthalene-6-sulfonate Tetradecylpyridine bromide Tris (hydroxymethyl) aminomethane Tetramethylrhodamine isothiocyanate Tetradecyltrimethyl ammonium bromide Uric acid U.S. Department of Agriculture-Food Safety and Inspection Service... 

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