Orbital shaker

Tomato cell suspensions in modified Murashige and Skoog medium (12) were assayed for change in cell volume at varying concentrations of polar and nonpolar extracts. Twenty-five ml erlenmeyer flasks (5 reps/treatment) containing 10 ml of sterile medium, tomato cell suspension, and extract were allowed to settle for 20 min in sidearm test tube attachments. Relative height of cell volume fraction to medium was recorded each day for 11 days. Cell suspensions were incubated in the dark at 27 C on an orbital shaker at 125 rpm. The doubling time of control cell suspensions were approximately 2.25 days. 

Mnk assay (using S6peptide as substrate) Each reaction contains 20 1 of immunoprecipitated or purified recombinant Mnk, as described previously, and S6 peptide (KEAKEKRQEQIAKKRRLSSLRASTSKSESSQK, final concentration = 200 pM) in kinase buffer. To initiate the reaction, 6 p of the following mix is added 5 pi 5x kinase buffer, 0.25 pi 10 mM ATP, 1 pCi [7 32P]ATP, and H20. Reactions are carried out in an orbital shaker at 30° and 900 rpm, for example, for 20 min (Mnkl) or for 5 min (Mnk2) and stopped by spotting 20 pi of the reaction mixture onto P81 paper. The filters are washed in 1% orthophosphoric acid three times. The radioactivity is determined by using a liquid scintillation counter. 

We perform equilibrium assays using an orbital shaker and incubation times of 20 h. The active flow of the fluid over the disc surface and the long incubation times ensure... 

After 1 hour, carbon dioxide should be evolved at approximately 12 bubbles/second. Alternatively the whole reaction may be carried out in a 2 L conical-flask placed in an orbital shaker at 30 °C and 220 r.p.m. 

After 1 h on an orbital shaker, pH is recorded and 5 mL aliquots of each thin slurry are filtered for ICP analysis. Over powders, Pt adsorption is complete within minutes [19,23], and 1 h contact is sufficient to ensure equilibration. Samples of the parent solution (before contact) are also filtered and analyzed with ICP, which can be used to determine not only metal uptake, but also dissolution of the support. The metal uptake, in terms of surface density (mol/m2), is calculated by dividing the concentration difference by the SL ... 

Each selected test strain is grown for 10 h at 37°C in nutrient broth (Oxoid No. 2) or supplemented minimal media (Vogel-Bonner) on an orbital shaker. A timing device can be used to ensure that cultures are ready at the beginning of the working day. 

To isooctane (25 mL) was added (7 ,5)-ibuprofen ester (0.27 g, 40 mM), 0.5 m sodium hydroxide (25 mL) and immobilized lipase (0.2 g). The reaction medium consisted of two layers of solution, namely ibuprofen ester in isooctane and aqueous NaOH, where the reaction only occurred at the interface between these two layers. The mixture was agitated in an orbital shaker at constant temperature of 45 °C and at 200 rpm. 

Reaction was initiated with 1 mg yeast ADH (663 U mg ) plus 10 /rg N145V and the mixture was held at 25 °C in an orbital shaker incubator. 

Add cell suspension on complexes by a single swift pipetting step and shake on an orbital shaker for 2-3 s at 400 rpm to equally distribute the cells throughout the well. 

Mix well (10 min) using an orbital shaker and proceed immediately to step 6. 

In in vitro permeability studies conducted in static systems, the UWL adjacent to the membrane can be up to 1500-4000 j,m thick, whereas in vivo the UWL is only 30-300 j,m in the GI tract and is negligible for the BBB [71]. The experimental system is often stirred or shaken to minimize the effects of the UWL. An orbital shaker is often not effective and can be modified by adding beads to enhance the agitation. Recently, it has been clearly demonstrated that the quantitative structure activity relationship was interfered if the UWL limited... 

A 1-L H2S-inert test container (glass test bottle) is filled to 50 vol % with fuel oil from a filled H2S inert container (glass sample bottle) just prior to testing. In the test container, the vapor space above the fuel oil sample is purged with nitrogen to displace air. The test container with sample is heated in an oven to 140°F (60°C) and agitated on an orbital shaker at 220 rpmfor three minutes. 

Figure 7.8. IR spectra taken from individual formylpolystyrene beads after 30 min with a two-fold excess of dansylhydrazine in DMF mixed using (A) a wrist shaker and (B) an orbit shaker with the reaction tube fixed at a 45° angle. The starting material has a band at 2728 cm-1 and the product at 2790 cm-1.

Add 2 mL of nutrient broth containing 10 mM MgS04 to the tubes, and shake them vigorously at 37°C in an orbital shaker for 4-6 h or until lysis occurs. Lysis is complete when the solution becomes clear and contains string-like cell debris. Comparing the appearance of the infected culture with the control makes it easy to determine when this point is reached. 

Culture bacteria and phage in liquid media at 37°C m an orbital shaker at 250-300 rpm... 

Place membrane in heat-sealable plastic bag with 5 ml blocking buffer and seal bag. Incubate 30 min to 1 hr at room temperature with agitation on an orbital shaker or rocking platform. 

Equilibrate membrane in appropriate blocking buffer in heat-sealed plastic bag with constant agitation using an orbital shaker or rocking platform. For nitrocellulose and PVDF, incubate 30 to 60 min at room temperature. For nylon, incubate >2 hr at 37°C. 

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