Luria broth

Enterococcus gallinarum Direct Black 38 The bacterium removed 53-63% of the dye in 24 h in minimal medium while 71-85% of decolourization was observed in Luria broth medium. [66]... 

Standard nutrient media for bacterial (Luria Broth Miller, Muller-Hinton Broth) and fungal growth (Sabouraud, yeast culture media). 

Grow 50 ml of an overnight culture (—18 hr) in Luria broth, NZYM, or Circle Grow medium (BIO 101 Labs, La Jolla, CA) with shaking (225 rpm). Harvest by centrifuging at 7000 g for 5 min. 

Inoculate (sterile technique) 100 ml of sterile Luria broth (200- to 400-ml flask) with a sample of wild-type Bacillus subtilis obtained from a stock culture slant. Incubate this solution on a shaker at 37°C for 15 to 24 hr (until the culture is densely turbid and is entering the stationary phase of growth). 

Luria broth—Luria broth Prepare five 100-ml and five 10-liter solutions containing 10 g/liter Bacto-Tryptone, 5 g/liter Bacto-Yeast extract, 5 g/liter NaCl, 0.5 g/liter antifoam agent (e.g., Polyglycol P-2000), and 1 g/liter glucose (autoclave separately). Autoclave the main solution and separate solution of concentrated glucose for 20 min at 105 to 110°C and cool use sterile conditions to add the glucose solution to the main solution and use within 24 hr. 

Inoculate a single colony into 3.0 mL of Luria broth with 100 pg/mL of ampicillin, and grow with shaking for 6-8 h at 37°C. 

Luria broth (LB) is available from BiolOl (La Jolla, CA). 

Transformed E. coli containing the plasmid of interest are grown from a glycerol stock (0.85 mL bacterial culture + 0.15 mL glycerol, stored at -70°C) and plated on a semisolid matrix containing Luria Broth (LB), agar, and the selection antibiotic. 

E. coli culture media luria broth (LB) medium and LB agar plate with 50 pg/mL kanamycin or 100 pg/mL ampicillin. Miller s LB powder (Invitrogen) or LB agar powder (Invittogen) is dissolved in distilled water and antoclaved. The media is cooled to approx 50°C before adding the antibiotic from a lOOOX stock solntion (kanamycin, 50 mg/mL or ampicillin, 100 mg/mL). 

The antibacterial activity of the fabric samples was tested using AATCC Method 100 using Staphylococcus aureus. The fabric swatch (l xl ) was placed in a sterilized flask containing Luria broth (Hi-media) solution, inoculated with 20 pi of test organisms and incubated at 37°C for 24 hrs in a laboratory shaker at 200 rpm. After incubation the number of colony forming unit (CFU) was counted by using serial dilution method. 

Luria Broth liquid media per liter, 10 g tryptone, 5 g yeast extract, 10 g NaCl, dissolved in deionized water and sterilized by autoclaving. 

Luria Broth agar plates (1.5 % agar) with Kanamycin (50 mg/L) and Rifampicin (25 mg/L). 

Grow suitably transformed A robacterium tumefaciens LBA 4404 cells in liqirid Luria Broth containing rifampicin and kanamycin see Note 1). Spread 100-200 iL of a stationary phase culture on Luria Broth agar plates containing rifampicin and kanamycin. Incubate at 28 °C for 2-3 days. 

Inoculate 50 ml Luria broth (LB) containing appropriate antibiotics with Agrobacterium. 

Grow up overnight, at 37°C, a 20-mL culture of transformed E. coli JMlOl, in freshly prepared Luria broth containing 40 pL of the ampicillin solution 2. Inoculate 1 L of Luna broth containing 2 mL of the ampicillin solution with the aforementioned culture Incubate for 60-80 min at 30°C until an absorbance of 0.2 at 600 nm is attained Add 5 mL of the nalidixic acid solution to induce the expression of CRBP and continue the incubation at 30°C up to an absorbance of 0.85/1 0 at 600 nm... 

Luria broth (LB) medium supplemented with 10% glycerol and 50 Xg/ml carbenicillin. 

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