Broth microdilution method

The MIC value ( ig/ml) was determined for each compound by a broth microdilution method using H. pylori (ATCC43504) [26,27], Each 69 and 70 each equivalent mixture of DL (R, S). 

A sensitive and simple chemiluminescent (CL) method for measuring antioxidant activity was developed. The determination of TEAC (Trolox equivalent antioxidant capacity) is based on the inhibition of CL intensity of luminol by an antioxidant. Antimicrobial activity was tested as a minimal inhibitory concentration (MIC) by broth microdilution method on gram-positive Enterococcus faecalis. Staphylococcus aureus) and gram-negative Pseudomonas aeruginosa, Escherichia coli) bacterial strains. 

In a recent study, with S. multicaulis, four of the new compounds (29-32) showed structural resemblence to tanshinone-type compounds. Therefore, they and the other new compounds (33-34 ) isolated from the same plant, were tested against bacterial strains (Table 28). All the compounds from this plant were also tested in Mycobacterium tuberculosis H37Rv test system. Their antituberculous activity was established according to broth microdilution method . When the MIC results were... 

In order to assess the bacterial susceptibility profile, the MIC for different drugs was established using the broth microdilution method [135]. The surface hydrophobicity of the bacterial strains was determined using the microbial adhesion to hydrocarbon... 

Antibacterial activity testing. Determined using the broth microdilution method. Incubations were performed for 18 h using an Ominolog automated incubator (Biolog Inc. 21124 Cabot Boulevard, Hayward, CA 94545, USA). 

Minimal inhibitory concentrations (MIC, mg/ml) were determined by a broth microdilution method adapted from the tentative reference method (document MJ7-T) recommended hy the Committee on Antifungal Susceptibility Testing (36). Antibiotic medium 3 was used rather than RPMI 1640 medium. The MIC is defined as the lowest concentration of compound chat completely snhihited growth when tested with LY303366, cilofungin, or amphotericin B. 

Weseler, A., H.K. Geiss, R. Sailer et al., 2005. A novel colorimetric broth microdilution method to determine the minimum inhibitory concentration (MIC) of antibiotics and essential oils against Helicobacter pylori. Pharmazie, 60 498-502. 

In contrast, agar dilution and broth microdilution methods are able to overcome some of the limitations of the disk diffusion method. Not only are they more convenient for routine antimicrobial susceptibihty testing of bacteria in clinical laboratories, they are capable of drawing quantitative conclusions by determining the MIC values for antimicrobials, as opposed to qualitative data generated by the disk diffusion method (Kim and Kim 2007). 

Liao, R. S. Reimie, R. P Talbot, J. A. Novel fluorescent broth microdilution method for fluconazole susceptibility testing of Candida albicans. J. Clin. Microbiol. 2001, 39, 2708-2712. 

Several honeybee products including RJ have been evaluated for their ability to inhibit the growth of 40 yeast strains of Candida albicans, Candida glabrata, Candida krusei, and Trichosporon spp. using the broth microdilution method. MIC values for RJ and fluconazole (used as standard) were 0.06-1 and 0.02-96 pg/mL, respectively, demonstrating that RJ could potentially control some fluconazole-resistant fungal strains [44]. lO-hydroxy-2-decenoic acid (10-HDA), main component of RJ, is well known for its antimicrobial... 

Tullio, V., A. Nostro, N. Mandras et al., 2006. Antifnngal activity of essential oils against lamentous fungi determined by broth microdilution and vapour contact methods. J. Appl. Microbiol., 102 1544-1550. 

Currently, several methods have been applied to measure the in vitro antimicrobial activity of lichens, such as disk diffusion, agar dilution, and broth microdilution (Fig. 3.1). 

Fig. 3.1 Disk diffusion (a), agar dilution (b), and broth microdilution (c) methods to measure the...

Tommasi L, Negro C, Miceh A et al (2009) Antimicrobial activity of essentieil oils from aromatic plants grown in the Mediterranean area. J Essent Oil Res 21 185-189 Tullio V, Nostro A, Mandras N et al (2007) Antifungal activity of essentitil oils against filamentous fungi determined by broth microdilution and vapour contact methods. J Appl Microbiol... 

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