Antifungal susceptibility testing

National Committee for Clinical Laboratory Standards, 1996, Method for Broth Dilution Antifungal Susceptibility Testing Yeast Approved Standard, M27-A, 15, 10, NCCLS, VA Medical Center, Tucson, AZ. 

National Committee for Clinical Laboratory Standards (1997) Reference method for broth dilution antifungal susceptibility testing of yeasts approved standard. NCCLS document M27-A, Wayne, PA. 

NCCLS (2002) Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts Approved Standard M-27-A2, 22 (15). National Committee on Clinical Laboratory Standards, Wayne, PA... 

Vella, A., Carolis, E.D., Vaccaro, L, Posteraro, P., et al. (2013) Rapid antifungal susceptibility testing by matrix-assisted laser desorption ionization time-of-flight mass... 

Liao, R. S. Rennie, R. P. Talbot, J. A. Comparative evaluation of a new fluoreseent carboxyfluoies-cein diacetate-modified microdilution method for antifungal susceptibility testing of Candida albicans isolates. Antimicrob. Agents Chemother. 2002, 46, 3236-3242. 

Minimal inhibitory concentrations (MIC, mg/ml) were determined by a broth microdilution method adapted from the tentative reference method (document MJ7-T) recommended hy the Committee on Antifungal Susceptibility Testing (36). Antibiotic medium 3 was used rather than RPMI 1640 medium. The MIC is defined as the lowest concentration of compound chat completely snhihited growth when tested with LY303366, cilofungin, or amphotericin B. 

Calgiani JN, Bartlett MS. EspineUlngrolf A, Ftomtling RA, Pfaller M, Rinaldi MG. Refer ence Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts Proposed Stan dard. NCCLS Document M27-T, Vol. 15, No. 10. Villanova, Pennsylvania NCCLS, 1995. 

TeUier, R. Ktajden, M. Grigoriew, G. A. Campbell, I. Innovative endpoint detennination system for antifungal susceptibility testing of yeasts. Antimicrob. Agents Chemother. 1992, 36, 1619—1625. 

Hoeprich and Huston [117] assessed the stability of miconazole and three other antifungal agents under conditions encountered in bioassay and susceptibility testing in vitro. Although the amphotericins were labile as compared with other drugs, tests should be reliable with all four drugs in view of the rapid action of the polyenes and the relatively slow action of miconazole and 5-fluorocytosines. 

Standardized methods for surveillance of antifungal drug susceptibility have been a recent development (Rex et al, 1993). The M27 protocol of the National Committee for Clinical Laboratory Standards (NCCLS) for testing of yeasts focused on laboratory to laboratory reproducibility and became an approved standard in 1997 (National Committee for Clinical Laboratory Standards, 1997). A modification of M27 for testing of molds has recently been proposed as NCCLS document M38-P (National Committee for Clinical Laboratory Standards, 1998). With these tools, collaborative studies to validate the predictive power of these results have been possible. Interpretive breakpoints for... 

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