Bromelain pineapple

C Bromelain Pineapple stems Peptides Carboxylic acid... 

Bromelain Pineapple Pharmaceutical anti-inllammatoiy bum debridement enhancement of dmg absorption... 

Another subclass of proteases attacks internal peptide bonds and Hberates large peptide fragments. Bromelain, a plant protease derived from the stem of the pineapple plant, can even produce detectable semm proteolysis after oral adrninistration (180). Oral therapy with bromelain significantly reduces bmising that stems from obstetrical manipulations (181). Bromelain—pancreatin combinations have been more effective in digestive insufficiency compared to either pancreatin or placebo (182,183). Bromelain may also enhance the activity of antibiotics, especially tetracycline, when adrninistered concurrently (184). 

Bromelain (anti-inflammatory Ananase from pineapple) [37189-34-7] Mr 33 000, [EC 3.4.33.4]. This protease has been purified via the acetone powder, G-75 Sephadex gel filtration and Bio-Rex 70 ion-exchange chromatography and has Aj 20.1 at 280nm. The protease from pineapple hydrolyses benzoyl glycine ethyl ester with a Km (app) of 210mM and kcat of 0.36 sec. [Murachi Methods Enzymol 19 273 1970 Balls et al. nd Eng Chem 33 950 1941.]... 

According to U.S. Patent 3,002,891, the following describes pilot plant production of bromelain. Stripped pineapple stumps were passed four times through a three roll sugar mill press. 

Bromelain (from pineapple) has applications in milk clotting. Malt extract from barley has amylolytic enzymes used in beer brewing. 

Bromelain—a plant protease with uses similar to those of papain. Bromelain is obtained from stumps left over from pineapple harvest. 

A family of cysteine proteinase inhibitors different from the cystatin super-family was Isolated from pineapple stem acetone powder. These inhibitors have a Mr of about 5800 and are composed of a longer (41 amino acids) and a shorter (1L ammo acids) peptide chain connected with disulfide bonds [29]. The conserved sequence Gin- fal-Val-AJa-Gly of the cystatins is not present in these inhibitors, indicating a different mechanism of interaction. The bromelain inhibitor VI was found to share similar folding and disulfide band connectivities with the Bowman-Bilk trypsiu/chymatrypsm inhibitor from soybean [30,31]. Hie physiological role of these inhibitors remains undear. 

S. I. Ttausrig and S. Bulkin. Bromelain, the enzyme complex of pineapple (Ananas oomosus) and Its clinical application. An update, J. EthnopkanttaeoL 22 191 (1988). 

R. M. Hdnicke and W. A. Gortner. Stem bromelain, a nrar protease preparation from pineapple plants. Econ. Hot. 11 225 (1957). 

K. Hatanc, M. Kqjima, M. Tanokura, and K, Takahaihi. Solution structure of bromelain inhibitor VI from pineapple stem structural similarity with Bawmnn-Birk hyprin/chymotrypsin inhibitor from soybean. Biochemistry J5 5379 (1996). 

F, Yamada, N. Takabashi, and T. Munchi. Purification and characterization of a proteinase from pineapple fruit, fruit bromelain FA2.X Bioctum (Tokyo) 79 1223 (1976). 

S. J, Taussig, J. SzefarcaeK, and S. Batkin. Inhibition of tumor growth in vitro by bromelain, an extract of ihc pineapple plant (Ananas camosus). rianra hied. 6 556... 

Enyzmes are present in all plant cells, and in greatest variety in seeds. Most plant enzymes are intracellular as they cannot diffuse through the cell wall. In a few cases the enzyme can be obtained from the plant juice, for example, the enzyme bromelain can be obtained from the stem of the pineapple. Otherwise, methods of extraction are used which depend on the rupturing of the cell wall, for example, grinding the plant with sand, either with or without freezing, or drying of the tissue. 

Bromelain The purified proteolytic substance derived from the pineapples Ananas comosus and Ananas bracteatus L. (Fam. Bromeliaceae). Produced as a white to light tan, amorphous powder soluble in water (the solution is usually colorless to light yellow and somewhat opalescent), but practically insoluble in alcohol, in chloroform, and in ether. Major active principle bromelain. Typical applications used in the chillproofing of beer, in the tenderizing of meat, in the preparation of precooked cereals, in the production of protein hydrolysates, and in baking. 

Bromelain protease pineapples Ananas comosus Ananas bracteatus (L) none 3.4.22.32 3.4.22.33... 

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