Brassica napus seed

Dandurand L M, Mosher R D and Knudsen G R (2000), Combined effects of Brassica napus seed meal and Trichoderma harzianum on two soilbome plant pathogens , Can... 

Enkephalins Promoter and terminator of 2S1 albumin gene of A. thaliana Integrated in 2S1 albumin of A. thaliana and flanked by tryptic cleavage sites A. thaliana (seed) Brassica napus (seed) 2.9% ofTSP, (200 nmol g 1) 50 nmol 3... 

Harada, J.J., De-Lisle, A.J., Bakden, C.S. Crouch, M.L. (1989). Unusual sequence of an abscisic acid-inducible mRNA which accumulates late in Brassica napus seed development. Plant Molecular Biology 12, 395-401. 

TAIPALENSUU, J., ERIKSSON, S., RASK, L., The myrosinase-binding protein from Brassica napus seeds possesses lectin activity and has a highly similar vegetatively expressed counterpart., Eur. J. Biochem., 1997,250,680-688. 

BJORKMAN, R., LONNERDAL, B., Studies on Myrosinases III Enzymatic properties of myrosinases from Sinapsis alba and Brassica napus seeds. Biochim. Biophy. Acta, 1973,327,121-131. 

Baumert, A., Milkowski, C., Schmidt, J., Nimtz, M., Wray, V. and Strack, D. (2005) Formation of a complex pattern of sinapate esters in Brassica napus seeds, catalyzed by enzymes of a serine carboxypeptidase-like acyltransferase family Phytochemistry, 66,1334- 5. 

The in vitro methods also include chemical mutation techniques with the use of, for example, of N-ethyl-N-nitrosourea and ethyhnethanesulfonate. These mutagenic agents enable the increase in the oleic acid concentration in Brassica napus seed oil to over 80% (MacKenzie, 1999) (Table 15.1). 

Eccleston VS, Ohlrogge JB (1998) Expression of lauroyl-acyl crirrier protein thioesterase in Brassica napus seeds induces pathways for both fatty acid oxidation and biosynthesis and imphes a set point for triacylglycerol accumulation. Plant Cell 10 613-621 Eccleston VS, Cranmer AM, Voelker TA, OUrogge JB (1996) Medium-chain fatty acid biosynthesis and utilization in Brassica napus plants expressing lauroyl-acyl crurier protein thioesterase. Planta 198 46-53... 

Yu B, Lydiate D, Young L, Schafer U, Hannoufa A (2008) Enhancing the carotenoid content of Brassica napus seeds by downregulating lycopene epsilon cyclase. Transgenic Res 17 573-585... 

Baumert, A. et al. (2005) Formation of a complex pattern of sinapate esters in Brassica napus seeds, catalyzed by enzymes of a serine... 

Rapeseed (low erucic acid) Brassica napus Seed 30-40... 

Acyl carrier protein (ACP) plays a central rOle In lipid metabolism, serving as both a component of plant fatty acid synthetase (1) and as a substrate/cofactor for complex lipid biosynthesis (2). The protein has been purified from a number of plant sources and Its amino acid sequence determined for the protein from both barley leaf (3) and spinach leaf (4) material. Both of these two previously mentioned sources of ACP have two detectable forms of the protein (5-6) whilst in seed material only one form has been detected (5). ACP has been shown, using immunological techniques, to be a developmentslly regulated protein In maturing soy bean seeds. The activity of the protein appearing just prior to lipid accumulation (7). Despite the Importance of this protein in lipid metabolism and the fact that seeds are a major site of lipid synthesis there Is no reported literature on the characterization of ACP from seed material. The present study was aimed at a detailed characterization of ACP from rape (Brassica napus ) seed ... 

OVER-EXPRESSION AND STUDY OF l -KETOACYL-[ACP] REDUCTASE FROM BRASSICA NAPUS SEED... 

Martinez-Rivas JM, Thomas NC, Chase D, Fawcett T, Slabas AR. cDNA cloning and overexpression of 3-oxoacyl-ACP reductase from Brassica napus seed. Grasas y Aceites 1993 44 119-120... 

ELONGATION SYSTEM INVOLVED IN THE BIOSYNTHESIS OF VERY LONG CHAIN FATTY ACIDS IN BRASSICA NAPUS SEEDS CHARACTERIZATION AND SOLUBILIZATION... 

Brassica napus seeds Drakkar variety were placed on two sheets of filter paper in Petri-dishes with either distilled water (control) or with 3, 6, 9, 12, 15 and 18 g/1 NaQ solutions in darkness at 25 1°C. Cotyledons were separated from embryonic axes for lipid analysis at two day intervals up to the 10th day of germination lipid extraction and fatty acid analysis have been described in previous paper [1]. Kinetic studies of (l- Q-acetate incorporation were carried out in the control and treated whole seedlings according to Zanouk et aL [2] methods. The results represent the mean of three experiments. 

In most plants the major product of fatty acid biosynthesis in plastids is oleic acid and a highly active oleoyl-ACP thioesterase has been described [1, 2]. In the seeds of California Bay Umbellularia californicd) however, a lauroyl-ACP oesterase has been found that is involved in the production of 12 0 [3]. Recently, a partial cDNA clone (YAP140) fi om Arabidopsis thaliana siliques was isolated [4]. This clone showed a high sequence identity with the California Bay thioesterase cDNA but lower similarity with the safflower oleoyl-ACP thioesterase cDNA [2]. A. thaliana seeds show a "normal" fatty acid pattern (6.9 mol % 16 0 3.1 % 18 0 14.4% 18 1 26.9% 18 2,21.2% 18 3, 2.2% 20 0, 20.6% 20 1) and they contain only trace amounts of 12 0 [3]. In addition, the thioesterase from extracts oiA. thaliana seeds is specific for oleoyl-ACP [3]. fri this study we address the question of whether the novels, thaliana cDNA encodes a fimctional thioesterase and we examine its substrate specificity and expression in different, thaliana tissues and in developing Brassica napus seeds. 

In order to analyze the expression of the TE 3-2 gene, antibodies against the recombinant TE 3-2 protein were raised in rabbits and used to probe Western blots with protein fiom different A. thaliana tissues. The TE 3-2 protein was detected in siliques, leaves and roots. As the TE 3-2 gene product presumably was involved in fatty acid synthesis, we also analyzed the expression of the thioesterase in developing Brassica napus seeds. The protein was expressed throughout seed development and peeked between 18 and 31 days after flowering. This expression pattern was si ar to other enzymes involved in fatty acid biosynthesis. 

Peng, Q., Hu, Y, Wei, R., Zhang, Y, Guan, C., Ruan, Y, Liu, C., 2010. Simultaneous silencing of FAD2 and FAEl genes affects both oleic acid and erucic acid contents in Brassica napus seeds. Plant Cell Rep. 29,317-325. 

---