Broth brain heart infusion

This stock solution is conveniently stored frozen at — 20°C in 20 ml and 80 ml amounts after checking for contamination using 1) Saboraud fluid medium at 31°C for 1 week (Appendix 4) 2) brain heart infusion broth at 37°C for 1 week (Appendix 4). (One check of each should contain calf serum). 

Various other broths for detecting bacterial and fungal contamination include brain heart infusion broth, tryptose phosphate broth and trypticase soy broth. These should be made up as per manufacturers (Oxoid Ltd. or Difco Labs. see Appendix 3) instructions, but some procedures are given in Appendix 4. 

Both batches of virus should be tested for bacterial contamination (Chapter 9) by inoculating an aliquot of virus into brain-heart infusion broth (leave at 37° C for one week) and into Saboraud fluid medium (32°C for one week) (Appendix 4). 

Although this procedure is necessary for production of samples of pure virions it is usually unnecessary if a viral preparation is only required for subsequent infections. In such circumstances the cells should be harvested aseptically and processed to step e, omitting the DNase and RNase. The debris from the disrupted cells is pelleted at 15,000g for 30 min and the supernatant used as a source of virus. It should be tested for bacterial contamination with brain-heart infusion broth and Saboraud fluid medium (Appendix 4). The virus should be stored at -70°C at about 1010 p.f.u./ml. 

Dissolve 37.0 g of brain heart infusion broth powder (Oxoid, Basingstoke) in 1 1 of distilled water. 

Minimal inhibitory concentrations (MIC) determined in brain-heart infusion broth containing 10 cells/ml. Minimal bactericidal concentrations (MBC) determined by subculture into broth. Figures given are averages of two tubes except that the readings from four tubes were averaged for the E. coli data. 

Bacillus subtilis cells were grown in 1 liter brain heart infusion broth for 36 hours, centrifuged,and the cells were washed 2 times with sterile phosphate buffer Ten ml of the washed cell suspension was added to each flask which contained 0.5 gms of carrier. After 3 hours of contact time, the carriers were washed 3 times and maintained at 8°C overnight. 

Streptococcus mutans (S. mutans) IFO 13955 was obtained from the Institute for Fermentation, Osaka, Japan. S. mutans IFO 13955, was cultured on BHI (Brain Heart Infusion) broth, and each isothiocyanate was added in 80% aqueous N,N-dimethylformamide (0.05 mL) or 80% aqueous ethanol solution (0.05 mL), and then added to the test tube containing 4.94 mL of BHI broth and 1% sucrose. The test tube was allowed to stand at an angle of 30 for 24-48 h at 37 C. The BHI broth was removed and the residue was dried. The weight of the residue was then determined. The sucrose dependent adherence of 5. mutans IFO 13955 was represented by the relative percentage compared with the adherence in the absence of isothiocyanate. All tests were run in triplicate and averaged. 

Anaerobic incubations similarly prepared from other media using anal sac inocula, were also tested. These media were Robertson s meat broth (RM), casein acid hydrolysate medium (CHY) and an egg yolk medium (EY) (fresh egg yolk/glucose/Difco brain-heart infusion broth 5/2/100 w/w/w). After incubation, all fermentations were stopped by the addition of excess solid sodium chloride, and maintained at 4 C until required for testing. 

Figure 1.3. Titration curves by weak acids with brain-heart infusion broth. Comparison with the predictions of the modei of Wiison et ai. [WiL 00], The points correspond to the experimentai measurements and the iines correspond to the predictions of the modei...

Robbins et al., 1974 Brain heart infusion broth medium After staining Hook after staining "Hook Very low... 

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