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Thiopropyl sepharose

The use of thiopropyl-Sepharose and boronic acid-agarose is an example of covalent chromatography, since relatively strong but reversible covalent bonds are formed between the affinity gel and specific macromolecules. [Pg.103]

FIG. 11. Synthetic scheme of a multifunctional molecular probe, N-(2-aminobenzoyl)cysteine hydrazide (compound 6 ABCh), and BlotGlycoABC bead derived by conjugation with Thiopropyl Sepharose 6B. This figure is adapted and arranged from ref. 88. [Pg.238]

Covalent chromatography The method described by Alouf and Geoffrey (1988) is followed with some modifications. A set of columns is used in tandem. The first is a Thiopropyl-Sepharose 6B column (about 1 ml/per I of culture) in the free thiol form. The second (about 2 ml/per I of culture) is activated with thiopyridine (if necessary, the gel is activated according to the manufacturer s instruction). The gel of the first column is converted to the reduced form by washing with... [Pg.251]

Brain heart infusion Bacto Profuse-Peptone No. 3 Ultrafiltration membrane (cut off 30kDa) Thiopropyl-Sepharose 6B Alkyl-Superose HR 10/110 column... [Pg.254]

Protoporphyrin. MgP was prepared as described by Hinchigeri et al. (10) and was covalently attached to Thiopropyl-Sepharose 6B (Phannacia) by a modification of the diazo-coupling procedure originally developed by Suttnar et al. (11). The gel contained... [Pg.2583]

Protocol 3 provides an example of inunobilization of Npys-peptide to thiopropyl-Sepharose (37). [Pg.235]

Thiopropyl Sepharose 6B 6% agarose Thiol compounds -c=o, -C=C. -N=N, -SH 20 pmol 45-165 AP Biotech... [Pg.337]

SOLUBLE PROTEIN WITH ACCESIBLE SH-GROUP THIOPROPYL-SEPHAROSE ... [Pg.61]

HiTrap NHS-activated HP (NHS- derivatized, cross-linked agarose) NHS-activated Sepharose 4 Fast Flow (NHS- derivatized, cross-linked agarose) Thiopropyl Sepharose 6B (Carboxy-derivatized, cross-linked agarose)... [Pg.516]

See other pages where Thiopropyl sepharose is mentioned: [Pg.76]    [Pg.37]    [Pg.103]    [Pg.103]    [Pg.72]    [Pg.237]    [Pg.238]    [Pg.473]    [Pg.509]    [Pg.354]    [Pg.921]    [Pg.111]    [Pg.235]    [Pg.236]    [Pg.59]    [Pg.61]    [Pg.62]    [Pg.464]   
See also in sourсe #XX -- [ Pg.6 , Pg.37 ]



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Sepharose

Thiopropyl

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