Cutinases fungal

The purified fungal cutinase fiom F. oxysporum f. sp. pisi was kindly provided by Prof. C.M.J. Sagt in Utrecht University. The commercial product of esterase firom Candida cylindracea was purchased fi om Boehringer Mannheim (Germany). The enzymatic degradation of DEP... 

Fig. 2. Time-course variation in the amount of chemical compounds produced during DEP degradation by fungal cutinase (10 mg 1 ) (a) and yeast esterase (100 mg F) (b), analyzed through GC/MS chromatography.

Isolation of Fungal Cutinases and their Molecular Properties... 

Fungal cutinase catalyzes hydrolysis of model substrates and in particular p-nitrophenyl esters of short chain fatty acids, providing a convenient spectro-photometric assay for this enzyme activity [101,102,116]. Hydrolysis of model esters by this cutinase showed the high degree of preference of this enzyme for primary alcohol ester hydrolysis. Wax esters and methyl esters of fatty acids were hydrolyzed at low rates. Alkane-2-ol esters were hydrolyzed much more slowly than wax esters and esters of mid-chain secondary alcohols were not... 

Fungal cutinases show no free SH groups but have 4 Cys residues, indicating that they are in disulfide linkage [119]. The reaction of the native enzyme with DTE was extremely slow but in the presence of SDS at its CMC rapid reduction could be observed [102]. Reduction of the disulfide bridge resulted in irreversible inactivation of the enzyme and the protein tended to become insoluble. CD spectra of cutinase in the 205-230 nm region, before and after DTE reduc-... 

The crystal structure of cutinase from F. solani f. pisi (Fig. 10) indicated that this fungal cutinase constitutes a separate class of enzyme that maybe regarded as a bridge between esterases and lipases in that the free cutinase has a well-de-... 

Apart from proteases, cutinases and lipases were demonstrated to hydrolyse PA. Hydrolysis of PA with a lipase (not further specified) was confirmed by using FTIR analysis and dye-binding assays [32]. Several reports have assessed the potential of cutinases for PA functionalisation, and a fungal cutinase from F. solani was successfully genetically engineered towards higher activity on a PA oligomer and PA [23-25]. 

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