Blood-brain barrier endothelial cells

A cell line is a continuously growing cell population, which is derived from tumor cells or from primary cells, which have been immortalized using appropriate vectors. There are several lines of blood-brain barrier endothelial cells available, for example, MBECs (mouse brain endothelial cells), RBE4 cells (rat brain endothelial cells, clone 4), PBMECs (porcine brain microvessel... 

Williams H, Neuhaus J, Kniesel U, KrauB B, Schmid E-M, Ocalan M, Farell C, Risau W (1980) Modulation of tight junction structure in blood-brain barrier endothelial cells. J Cell Sci 107 1347-1357... 

Claudio, L., et al. 1990. Increased vesicular transport and decreased mitochondrial content in blood-brain barrier endothelial cells during experimental autoimmune encephalomyelitis. Am J Pathol 135 1157. 

Jodoin J, Demeule M, Fenart L, et al. P-glycoprotein in blood-brain barrier endothelial cells interaction and oligomerization with caveolins. I Neurochem 2003 87(4) 1010-23. 

Descamps L, Dehouck M-P, Torpier G, Cecchelli R. 1996. Receptor-mediated transcytosis of transferrin through blood-brain barrier endothelial cells. Am. J. Physiol. Heart Circ. Physiol. 39 1149-58... 

Lippmann ES, Azarin SM, Kay JE, Nessler RA, Wilson HK, Al-Ahmad A, Palecek SP, Shusta EV (2012) Derivation of blood-brain barrier endothelial cells from human pluripotent stem cells. Nat Biotechnol 30(8) 783-791... 

Lippoldt A, Kniesel U, Liebner S, et al. Structural alterations of tight junctions are associated with loss of polarity in stroke-prone spontaneously hypertensive rat blood-brain barrier endothelial cells. Brain Res 2000 885 251-261. 

Tripathi, A. K., Sullivan, D. J., and Stins, M. F. (2007). Plasmodium falciparum-infected erythrocytes decrease the integrity of human blood-brain barrier endothelial cell mono-layers. J. Infect. Dis. 195,942-950. 

Plateel, M., Dehouck, M.P., Torpier, G., Cecchelh, R., and Teissier, E., Hypoxia increases the susceptibility to oxidant stress and the permeabihty of the blood-brain barrier endothelial cell monolayer, /. Neurochem., 65(5), 2138,1995. 

Fig. 1 Anatomical makeup of the blood-brain barrier. Endothelial cells of the cerebral capillaries are tightly joined by complexes of tight junctions. Pericytes are found surrounding the capillary endothelium and are continuous with the basement membrane of the endothelium. The astrocytic endfeet are wrapped tightly around both pericytes and endothelial cells. Together, these cells work together to tightly regulate the influx/efflux and metabolism of molecules important for optimal brain function. Figure was reprinted, with permission [243]...

Liebner S, Kniesel U, Kalbacher H, Wolburg H. Correlation of tight junction morphology with the expression of tight junction proteins in blood-brain barrier endothelial cells. Eur J Cell Biol 2000 79 707-717. 

Zhang H, Mitin A, Vinogradov SV (2009) Efficient transfection of blood-brain barrier endothelial cells by lipoplexes and polyplexes in the presence of nuclear targeting NLS-PEG-acridine conjugates. Bioconjug Chem 20 120-128. doi 10.1021/bc8003414... 

Janigro, D., West, G. A., Nguyen, T. S., and Winn, H. R. (1994). Regulation of blood-brain barrier endothelial cells by nitric oxide. Circ. Res. 75, 528538. 

FIGURE 14.16 Schematic view of the blood-brain barrier. Endothelial cells form a continuous layer covering the inner surface of blood microvessels. At the point of contacts with brain tissues, these endothelial cells interact with pericytes and astrocytes. Nerve ending with specific neurotransmitters can also be observed. The light junctions (TJ) that link endothelial cells act as a physical barrier that blocks the paraceUular route and forces the molecular traffic to take the transceUijlar pathway (double arrows). 

Martins, T., Burgoyne, T, Kenny, B.-A., et al., 2013. Methamphetamine-induced nitric oxide promotes vesicular transport in blood-brain barrier endothelial cells. Neuropharmacology 65, 74-82. 

Miller, D., B. Keller, and R. Borchardt. 1994. Identification and distribution of insulin receptors on cultured bovine brain microvessel endothelial cells Possible function in insulin processing in the blood-brain barrier. J Cell Physiol 161 333. 

Transporters are critical in the function of capillary endothelium, where they contribute to the blood-brain, blood-germinal epithelium (blood-testis and blood-ovary), and blood-placental barriers. Endothelial cells in each of these tissues express high levels of MDR-1. The existence of a blood-brain barrier is well established and is thought to arise... 

Orte, C.. Lawrenson, J.G, Firm, T.M, Reid, A.R and Allt, G, (1999) A comparison of blood—brain barrier and blood—nerve barrier endothelial cell markers. Anatomy and Embryology, 199, 509-517. 

Hurst, R.D. and Fritz, LB., Properties of an immortahsed vascular endothelial/glioma cell co-culture model of the blood-brain barrier, J. Cell Physiol., 167(1), 81, 1996. 

The blood-brain barrier (BBB) forms a physiological barrier between the central nervous system and the blood circulation. It consists of glial cells and a special species of endothelial cells, which form tight junctions between each other thereby inhibiting paracellular transport. In addition, the endothelial cells of the BBB express a variety of ABC-transporters to protect the brain tissue against toxic metabolites and xenobiotics. The BBB is permeable to water, glucose, sodium chloride and non-ionised lipid-soluble molecules but large molecules such as peptides as well as many polar substances do not readily permeate the battier. 

MV Shah, KL Audus, RT Borchardt. The application of bovine brain micro vessel endothelial-cell monolayers grown onto polycarbonate membranes in vitro to estimate the potential permeability of solutes through the blood-brain barrier. Pharm Res 6 624-627, 1989. 

Miller DW, KL Audus, RT Borchardt. (1992). Cultured bovine brain microvessel endothelial cells A model of the blood brain barrier. J Tiss Cult Meth 14 217-224. 

Blood-brain barrier A physicochemical barrier formed by astrocytes and capillary endothelial cells. It prevents toxic chemicals from entering CNS neurons from the systemic blood circulation. 

Fig. 15.2 Diagram showing a transverse cross-section of a cerebral capillary. The endothelial cells, responsible for the main barrier properties of the blood-brain barrier are separated from the astrocyte foot processes, pericytes and occasional neurons by the basement membrane. All these components make up the blood-brain barrier.

Fig. 15.3 D iagram showing a longitudinal cross-section of the blood-brain barrier, with the brain capillary endothelial cells sealed by the tight junctions and surrounded by pericytes and astrocyte foot processes. These cellular components of the BBB are separated by a basement membrane.

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