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Biosensors enzyme-based electrochemical

An overview concerning the enzyme-based electrochemical biosensors published during the last 5 years for heavy-metal determinations is reported. Their sensitivity and selectivity toward inhibitors and the factors (pH, buffer, enzyme, and inhibitor concentrations) affecting the analytical characteristics of these biosensors are also discussed. [Pg.301]

Coupling between a biologically catalyzed reaction and an electrochemical reaction, referred to as bioelectrocatalysis, is the constructional principle for enzyme-based electrochemical biosensors. This means that the flow of electrons from a donor through the enzyme to an acceptor must reach the electrode in order for the corresponding current to be detected. In case a direct electron transfer between the active site of an enzjane and an electrode is not possible, a small molecular redox active species, e.g. hydrophobic ferrocene, meldola blue and menadione as well as hydrophilic ferricyanide, can be used as an electron transfer mediator. This means that the electrons from the active site of the enzyme reduce the mediator molecule, which, in turn, can diffuse to the electrode, where it donates the electrons upon oxidation. When these mediator molecules are employed for coupling of an enzymatic redox reaction to an electrode at a constant potential, the resulting application can be referred to as mediated amperometry or mediated bioelectrocatalysis. [Pg.410]

Everett W. R. and Rechnitz G. A., Enzyme-based electrochemical biosensors for determination of organophosphorous and carbamate pesticides, Anal. Lett., 32, 1-10, 1999. [Pg.331]

DNA and Enzyme-Based Electrochemical Biosensors Electrochemistry and AFM Surface Characterization... [Pg.105]

Enzyme-Based Electrochemical Biosensors for Measuring Relatively Small Blomolecules... [Pg.158]

Zhao, Z., Jiang, H., 2010. Enzyme-based electrochemical biosensors. In Serra, P.A. (Ed.), Biosensors. INTECH, Croatia. [Pg.98]

Enzymatic biosensors can be defined as an analytical device having an enzyme as a bioreceptor integrated or intimately associated with the physical transducer to produce a discrete or continuous digital electronic/optical signal that is proportional to the concentration of analyte present in the sample. This chapter describes the enzyme-based electrochemical biosensors for the measurement of clinically important biomatkers, beginning with a history of biosensors. [Pg.135]

Table 11.1 Representative summary of enzyme-based electrochemical biosensors for pesticides and phenols reported in the past 10 years (2003-2013)... [Pg.292]

Zhiwei Z, Helong J (2010). Enzyme-based electrochemical biosensors, biosensors, hr Serra PA (ed.), ISBN 978-953-7619-99-2, InTech, available frran http //www.intechopeiLctHn/ books/biosensOTs/enzyme-based-electrochemical-biosensors... [Pg.563]

Particularly attractive for numerous bioanalytical applications are colloidal metal (e.g., gold) and semiconductor quantum dot nanoparticles. The conductivity and catalytic properties of such systems have been employed for developing electrochemical gas sensors, electrochemical sensors based on molecular- or polymer-functionalized nanoparticle sensing interfaces, and for the construction of different biosensors including enzyme-based electrodes, immunosensors, and DNA sensors. Advances in the application of molecular and biomolecular functionalized metal, semiconductor, and magnetic particles for electroanalytical and bio-electroanalytical applications have been reviewed by Katz et al. [142]. [Pg.340]

Electrochemical biosensors are analytical devices in which an electrochemical device serves as a transduction element. They are of particular interest because of practical advantages, such as operation simplicity, low expense of fabrication, and suitability for real-time detection. Since the first proposal of the concept of an enzyme-based biosensor by Clark, Jr [1], significant progress in this field has been achieved with the inherited sensitivity and selectivity of enzymes for analytical purposes. [Pg.555]

The determination of H202 is very important in many different fields, such as in clinical, food, pharmaceutical, and environmental analyses [202], Many techniques such as spectrophotometry, chemiluminesence, fluorimetry, acoustic emission, and electrochemistry methods have been employed to determine H202. Electrochemical methods are often used because of their advantages. Among these electrochemical methods, the construction of the mediator-free enzyme-based biosensors based on the direct electrochemistry of redox proteins has been reported over the past decade [203— 204], The enzyme-based biosensors, which use cyt c as biocatalyzer to catalyze H202, were widely studied. [Pg.574]

Chapters 1 to 5 deal with ionophore-based potentiometric sensors or ion-selective electrodes (ISEs). Chapters 6 to 11 cover voltammetric sensors and biosensors and their various applications. The third section (Chapter 12) is dedicated to gas analysis. Chapters 13 to 17 deal with enzyme based sensors. Chapters 18 to 22 are dedicated to immuno-sensors and genosensors. Chapters 23 to 29 cover thick and thin film based sensors and the final section (Chapters 30 to 38) is focused on novel trends in electrochemical sensor technologies based on electronic tongues, micro and nanotechnologies, nanomaterials, etc. [Pg.1]

This book on Electrochemical (Bio)Sensor Analysis, edited by S. Alegret and A. Merko< i, is an additional step to advance the field of rapid analysis. It presents advanced sensor developments as well as practical applications of electrochemical (bio)sensors in various fields in a single source. The book contains 38 chapters grouped into seven sections (a) Potentiometric sensors, (b) Yoltammetric (bio)sensors, (c) Gas sensors, (d) Enzyme based sensors, (e) Affinity biosensors, (f) Thick and thin film biosensors, and (g) Novel trends. This interdisciplinary book has contributions from well-known specialists in the field and will be a useful resource for professionals with an interest in the application of electrochemical (bio)sensors. [Pg.3]

M. Campas, D. Szydlowska, M. Trojanowicz and J.-L. Marty, Enzyme inhibition-based biosensor for the electrochemical detection of microcystins in natural blooms of cyanobacteria (2006), submitted for publication. [Pg.354]

The concentration of analyzed substance (glucose) in this electrochemical system is determined by recording the 02 or H202 reduction current. The design of many commercial biosensors is based on these alternatives. It should be noted that enzymes adsorbed on a solid surface preserve their structure and activity. [Pg.291]

Enzyme sensors can measure analytes that are the substrates of enzymatic reactions. Thermometric sensors can measure the heat produced by the enzyme reaction [31], while optical or electrochemical transducers measure a product produced or cofactor consumed in the reaction. For example, several urea sensors are based on the hydrolysis of urea by urease producing ammonia, which can be detected by an ammonium ion-selective ISE or ISFET [48] or a conductometric device [49]. Amperometric enzyme sensors are based on the measurement of an electroactive product or cofactor [50] an example is the glucose oxidase-based sensor for glucose, the most commercially successful biosensor. Enzymes are incorporated in amperometric sensors in functionalised monolayers [51], entrapped in polymers [52], carbon pastes [53] or zeolites [54]. Other catalytic biological systems such as micro-organisms, abzymes, organelles and tissue slices have also been combined with electrochemical transducers. [Pg.422]


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