Biomolecule Detection

Biosensing Demonstrations with the OFRR 14.5.1 Quantitative Biomolecule Detection... 

The fundamental fluorescence turn-on sensor described above for biomolecule detection can be used in many different formats for homogeneous assays. For example, one attractive formulation is a reverse assay (Fig. 2) wherein a molecule similar in structure and function to the ligand is sensed by having the fluorescent polymer and the QTL bioagent complex together. In this case,... 

Kim NH, Baek TJ, Park HG, Seong GH (2007) Highly sensitive biomolecule detection on a quartz crystal microbalance using gold nanoparticles as signal amplification probes. Anal... 

Zhu H, White IM, Suter JD et al (2007) Analysis of biomolecule detection with optofluidic ring resonator sensors. Opt Express 15 9139-9146... 

Bonnin, C., Matoga, M., Gamier, N. et al. (2007) 224 nm deep-UV laser for native fluorescence, a new opportunity for biomolecules detection. J Chrom A, In Press. 

In recent years, amperometric-based microfluidic sensor has been demonstrated as a highly sensitive method for biomolecules detection. Combination of microfluidic techniques and amperometric component enables dynamic monitoring of the target molecules. 

Biosensors Using Magnetics, Fig. 1 Schematic of magnetically labeled biomolecule detection in a biosensor. Target bimnolecules bound with a magnetic particle interact with magnetraesistive sensor-bound counter biomolecules to be detected... 

N. V. C. Ralston, C. D. Hunt, Diadenosine phosphates and S-adenosylmethi-onine novel boron binding biomolecules detected by capillary electrophoresis, Biochimica Et Biophysica Acta - General Subjects 2001,1527, 20. 

Small molecules such as dopamine,ascorbic acid, amino acids, nucleotides, NADH, and glucose have been successfully detected with QD-based biosensors. FRET-based fluorescence microscopy and electrochemistry are the most commonly used methods for small biomolecule detection using QD-based biosensors. ... 

Macropores coated with (immobilized) biomolecules Detection of specific biomolecules, DNA, ete. (Bengtsson et al. 2000 Yoo et al. 2013)... 

Table 1 Moderate-size biomolecule detection by different PSi optieal transducers ...

Binding assays for small molecules detection 3 Effective optical thickness (EOT) 3-4 Moderate-size biomolecules, detection and recognition 4 Organophosphorus compounds (OPCs) 4 Photoluminescence-based transducers 2 PSi nanostructures-sensing concept 3-4 Reflectance-based transducers 2 Sensing of complex samples 5... 

A biosensor s detector typically consists of chemical receptors attached or immobilized to a material surface (typically the transducer surface). These are often themselves biomolecules. Detection involves an interaction between these immobilized molecules and biomolecules from solution that approach the detector surface. In this sense, both the fabrication of, and detection using, biosensors involve biomolecules at interfaces. 

Figure 16 Biomolecule detection using magnetic relaxation switches. The analyte-induced aggregation of MNPs dephases the spins of the surrounding water protons more efficiently than MNPs present in the individual state. The effect is observed as a decrease in spin-spin relaxation time (T2).

Excellent recent reviews on graphene-based electrochemical sensors [113] and on analytical devices in biomolecules detection and cancer diagnostics [114] appeared recently, where the superior electrochemical sensing performances of graphene-based electrodes toward the detection of various biomolecules and chemicals have been demonstrated. Also in this field, porphyrin-functionalized graphene hybrids resulted in interesting analytical applications, as will be discussed next. 

Metal Nanoparticles as Labels in Biomolecule Detection Strategies.491... 

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