Biogenic producing bacteria, detection

Landete, J.M., de las Rivas, B., Marcobal, A. Munoz, R. (2007a). Molecular methods for the detection of biogenic amine-producing bacteria on foods. Int. J. Food Microbiol., 117, 258-269. 

Techniques for the detection of biogenic amine-producing bacteria... 

CS2 and COS occur in the atmosphere in significant amounts - CS2, 3.8-4.7 Tg S yr-1, COS, 2.7-3.5 Tg S yr-1 one-fifth to one quarter of these amounts are probably anthropogenic. Biogenic CS2 mainly originates in marine settings - anaerobic sediments (bacteria) and salt marshes with a role for Spartina alterniflora.6,10 Some terrestrial plants produce CS2 and tree roots are another source, usually after cutting or wetting. A tree of central America, Stryphnodendron excelsum. can be detected by its CS2 odor.10... 

A recombinant IFN-P, IFN-pia (Rebif Serono and Avonex Biogen) is produced for commercial use in Chinese hamster ovary (CHO) cells. A synthetic mutant produced in bacteria has a substitution of serine for cysteine at amino acid 17, yielding IFN-pib Betaseron Berlex). Both IFN-pia and IFN-pib are approved by the FDA for treatment of multiple sclerosis (MS) and have shown comparable biological activity (see Section 7.3). In vivo IFN-a2 and IFN-alb show comparable biological activity as well as similar side effects [54,55]. However, IFN-P is eliminated faster, resulting in no detectable serum peak levels [56]. The clinical consequence of this is not known. Objective responses, whether partial or complete tumor regression, have been documented in patients with carcinoma of the breast, hairy-cell leukemia, and non-small-cell lung cancer [57,58]. 

Thin-Layer Chromatography (TLC) is a rapid and simple technique to detect several amines. This was one of the first techniques used to determine biogenic amines in foods (Halasz et al. 1994). Recently, a simple and rapid qualitative TLC method to determine the ability of bacteria to produce biogenic amines in liquid culture media containing the amino acid precursor was described by Garci a-Moruno et al. (2005). 

Recently, multiple PCR systems have been published that use different pairs of primers to simultaneously detect lactic acid bacteria producers of histamine, tyra-mine and putrescine, the majority wine biogenic amines (Marcobal et al. 2005b Constantini et al. 2006). 

Marcobal, A., De las Rivas, B., Munoz, R. (2006). Methods for the detection of bacteria producing biogenic amines on foods a survey. Journal of Consumer Protection and Food... 

Sciancalepore, A. G., Mele, E., Arcadio, V., Reddavide, F., Grieco, F, Spano, G., et al. (2013). Microdroplet-based multiplex PCR on chip to detect foodborne bacteria producing biogenic amines. Food Microbiology, 35,10-14. http //dx.doi.Org/10.1016/j.fm.2013.02.010. 

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