Biochemical experiment

How do the Predicted Free Energy Changes Fit With the Revised Model Biochemical Experiments with Fibers... 

A variety of different methodologies has been employed to investigate the neurotransmitter systems involved in control of behavioral states. Biochemical experiments have elucidated the pathways and enzymes involved in the synthesis, degradation, release and reuptake of different neurotransmitters. Immuno-histochemical techniques have allowed the visualization of their cellular and sub-cellular distribution throughout the nervous system as well as the distribution of their receptors and uptake systems. Chemical sampling techniques, including... 

Results of a variety of neurophysiological and biochemical experiments suggest the presence of two distinguishable... 

For a complete functional study of a biological pathway, it is often necessary to confirm the important protein interactions by in vivo experiments. This can be done by demonstrating protein localizations on a microscopic level, for instance, by tagging proteins with the green fluorescent protein or localizing them with antibodies and colloidal gold particles using an electron microscope. Additional, very specific biochemical experiments are often required to confirm the putative protein function. 

It is possible to identify several apparently disconnected themes which led ultimately to the analysis of the chemistry of living cells. Perhaps the first biochemical experiment in Europe may have been that of van Helmont (1579-1644), a physician with a profound interest in chemistry. He grew a willow tree in a weighed amount of earth, watered it for five years and then weighed the earth and the tree again. The... 

The Xenopus oocyte can reliably express LGIC receptors. In our laboratory, we have seen robust expression of the Torpedo nAChR, 5-HT3 receptors and various GABAa receptor subtypes in oocytes. Injection of cRNA transcripts is a convenient and reproducible way to achieve the expression levels needed for functional analysis of receptor subtypes. We have found that functional characterization with this system complements biochemical experiments conducted on native receptors or those that have been expressed in mammahan cells. A combination of these approaches is essential for furthering our understanding of structure-function relationships in these receptors. 

The structurally related myxochromides Aj.j are cyclic hexapeptides produced by several Myxococcus species. These examples contain a proline residue, which is not present in myxochromides Si 3, as the fourth amino acid in their peptide core. The NRPSs responsible for myxochromides A and S biosynthesis have exacdy the same module and domain organization thus, the fourth module of the myxochromide S synthetase must be skipped to account for the natural product. Biochemical experiments revealed that the A domain of this module activates L-proline, but the adjacent PCP domain cannot be phosphopantetheinylated by a PPTase. These results suggest that the C domain of module 5 reacts directly with the tripeptide intermediate bound to the PCP domain of module 3 in myxochromide S biosynthesis. A similar example of domain skipping has been noted in the biosynthesis of the mannopeptimycins. ... 

Hammerhead construct RNA 6 16 nt ribozyme, 25 nt substrate. Used for Scott group crystal structures and biochemical experiments by Hampel and Burke, references 33, 56 5 3 ... 

Used in biochemical experiments. Two representational methods shown. 

Stabihty. The buffer should be stable under the conditions of the biochemical experiment or enzyme assay. 

Domains may be regarded as the basic units for the structure, function and evolution of proteins, but the definition of a domain remains fuzzy. They are most often treated as compact or connected areas that are apparent from a visual inspection of protein models. To avoid subjectivity and ambiguities of visual inspection, computer algorithms have been developed to localize domains. Rashin offered an alternative interpretation domains are stable globular fragments, generated in biochemical experiments that refold autonomously and retain specific functions. He proposed a method for localiz-... 

The half-lives of radioisotopes typically used in biochemical experiments are ... 

Describe your experiments in sufficient detail to ensure that others can replicate your findings. Avoid codes or your own jargon, unless you have clearly defined the term in writing. Many biochemical experiments involve many operations that, while combined in different ways, should be clearly described in their order of execution for each experiment. 

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