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Bean second-intemode bioassay

In 1970, Mitchell and colleagues reported that a lipoidal extract obtained from rape (Brassica napus L. ) pollen elicited strong elongation activity in the bean second-intemode bioassay (1). The activity was distinguished from other known plant hormones in that the lipoidal extract promoted not only cell elongation, but also cell division. The activity was termed brassins activity and extracts showing the same type of activity were also obtained from pollens of other plants (see ref. 2). Thus they proposed that new types of lipoidal plant hormone coined the brassins were contained in pollen. [Pg.29]

Since the isolation of BL, BL and its related compounds have been tested by a number of bioassays originally designed for known plant hormones. BRs have been shown to have a broad spectrum of biological activities (3,4,8). Structure-activity relationship of BRs has also been clarified by bean second-intemode bioassay, bean first-intemode bioassay, raphanus test, tomato test, and rice-lamina inclination test (25-57). [Pg.112]

The bean second-intemode bioassay was used for the isolation of BL from the pollen of rape (Brassica napus L.) (7). The procedure of the bioassay is described in... [Pg.112]

In subsequent years, John W. Mitchell developed two bioassays, popularly known as the bean first intemode and the bean second internode bioassays. The first intemode test was successfully used for screening several commercial chemicals, including such popular pesticidal chemicals as 2,4-D, 2,4,5-T, and Amo 1618. The bean second intemode bioassay did not find immediate application, although gibberellins, including gib-berellic acid (GA showed elongating properties in this test. [Pg.320]

Barley field experiments, description for brassin evaluation, 12 Barley leaf cell ultrastructure, protective effect of brassinosteroids under saline stress, 150,152/153,154/ Bean first-intemode bioassay, 321 Bean second-intemode bioassay activities of compounds, 65,68,69-7Of application, 321 brassin purification, 3-4 brassinosteroids, description, 109-110 development, 321 scheme, 63,65,66/... [Pg.345]

The development of bioassays for isolating bioactive compounds from natural sources has played an important role in recent smdies of namral BR phytochemistry. The development of highly sensitive and specific bioassays was essential for the isolation and purification of BRs from plant tissues because of the very low physiological concentrations of these hormones. The bean second intemode assay was used to isolate BL from rape pollen [1], and the rice-lamina inclination test was used to isolate CS from chestnut insect galls [14]. Following the publication of these results, the rice-lamina inclination test has been widely used to isolate many BRs from various plant sources because of its simplicity, high sensitivity, and specificity for BRs [25]. [Pg.4742]

The physiological concentrations of BRs in plants are extremely low (ng.Kg Fw), and it can be veiy difficult to analyze their abundance in plant tissues. A wide range of methods are currently employed for the determination and quantification of brassinosteroids in plants, including bioassays, diverse chromatographic procedures, radioimmunoassays [9], and enzyme-linked immunosorbent assays [10-13]. The most widely used bioassays are the second bean intemode bioassay and rice-lamina inclination test. These bioassays have been used in the isolation of brassinolide from rape pollen [1] and castasterone from chestnut insect galls [14]. Gas chromatography-mass spectrometry (GC-MS) analysis is the current standard technique for instrumental analysis of BRs [15-17]. [Pg.4737]


See other pages where Bean second-intemode bioassay is mentioned: [Pg.112]    [Pg.320]    [Pg.198]    [Pg.79]    [Pg.4169]    [Pg.112]    [Pg.320]    [Pg.198]    [Pg.79]    [Pg.4169]    [Pg.110]    [Pg.111]    [Pg.334]    [Pg.277]    [Pg.70]    [Pg.113]    [Pg.4230]    [Pg.87]   
See also in sourсe #XX -- [ Pg.63 , Pg.65 , Pg.66 , Pg.67 , Pg.68 , Pg.69 , Pg.109 ]




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