And automated image analysis

A new suspension array concept based on sedimentation and microscopic imaging was introduced by Moser et al. [98], Magnetic microbeads settle to the bottom of a microplate well by magnetic forces and form randomly ordered arrays, which are examined by fluorescence microscopy and automated imaging analysis. Each bead carries specific capture molecules and can be identified by a defined luminescent code. 

Straszheim, W.E. Markuszewski, R. Application of Scanning Electron Microscopy and Automated Image Analysis for Characterization of Mineral Matter in Coal, Am. Chem. Soc. Div. Fuel Chem. Preprints, 1985, 30(1), 47-55. 

CellProfiler and CellProfiler Analyst are free Open Source software for automated image analysis, data visualization, and machine learning. Versions for Mac, Windows, and Linux are available and both software can be downloaded at http //www.cellprofiler. org. CellProfiler was developed by Anne Carpenter and Thouis Jones in the laboratory of David Sabatini at the Whitehead Institute for Biomedical Research and by Polina Golland at the CSAIL of the MIT. 

Pressman, NJ. Wied, G.I. (1979). The automation of cancer cytology and cell image analysis. Ed., Proc. Second Inti. Conf., Chicago, IL. 

Here we address a problem which is commonly encountered in microscopy studies of particulate materials Given a collection of similar objects, such that the individual shapes occlude each other, how can the symmetry of these objects be extracted using methods of automated image analysis. The method we apply to solve the problem is to evaluate the symmetry of the occluded shapes, locate the center of symmetry, and reconstruct the symmetric shape most similar to the unoccluded original. 

Automatix produces automated image analysis packages comprising data collection, spreadsheet analysis and charting to be used with the Macintosh computer. This has been used, in conjunction with ancillary equipment, to produce a computer digital analysis system [137] for around 4,000 [138]. Later, a more sophisticated system, costing around 20,000, was described for digital examination of film. 

To be confident of an average size analysis within 10%, approximately 150 particles should be sized. To increase the confidence level to 5%, approximately 740 particles should be sized (104). Of course these numbers are only a rough guide, and the actual confidence levels will depend upon the nature of the size distribution. Figure 21 shows a problem that can occur when too much reliance is placed on automated image analysis, namely, inaccurate sizing of droplets that are slightly out of focus. The fields of view to be analyzed, either manually or with automated methods, have to be chosen carefully. 

Decordier I, Papine A, Plas G, et al. Automated image analysis of cytokinesis-blocked micronuclei An adapted protocol and a validated scoring procedure for biomonitoring. Mutagenesis. 2009 24(l) 85-93. 

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