Allogeneic cells

Selection. Products designed for positive or negative selection of autologous or allogenic cells intended for therapy (e.g., purging of tumor from bone marrow, selection of CD34+ cells). 

Mitchell MS, Harel W, Kan-Mitchell J, et al. Active specific immunotherapy of melanoma with allogeneic cell lysates. Rationale, results, and possible mechanisms of action. Ann NY Acad Sci 1993 690 153-166. 

Kaplan HJ, Streilein JW, Stevens TR (1975) Transplantadon immunology of die anterior chamber of die eye II. Immune response to allogeneic cells. J Immunol 115 805-810. 

If it is necessary to inject allogenic cells along with matrigel the test can be modified using nude mice. Nude mice usually exhibit a lower angiogenic response as compared to C57/black mice it is necessary to titrate the cytokines and heparin concentration. 

Cytotoxic lymphocyte function (CTL) [50] Allogeneic cells stimulate rodent splenocytes (e.g., P815 murine mastocytoma cell line) or human blood lymphocytes (e.g., Jurkat leukemic cell line) for killing by CTL. Rarely used for in vitro immunotoxicity... 

Whole melanoma cells Autologous cells Allogeneic cells Haptenized cells Melanoma cell lysates Viral oncolysates Shed melanoma cell supernatant Defined antigen vacciness Gangliosides (GM2, GD2)... 

Bonnet, M., Lespinats, G., Burtin, C. (1984). Histamine and serotonin suppression of lymphocyte response to phytohemagglutinin and allogeneic cells. Cell. Immunol, 83, 280—291. 

CD8+ cytotoxic T-cells (CTL) bind to virally infected, tumour or allogenic cells and induce apoptosis in these target cells via a mechanism that is not prevented by inhibitors of protein and RNA synthesis (Duke et al., 1983 Ostergaard and Clark,... 

The heterogeneity of each of the five carbohydrate components of human-serum a j-glycoprotein has been examined. A function of this glycoprotein might be to inhibit certain lymphocyte responses, including blast transformation induced by mitogens and allogeneic cells. ... 

Cultured Skin Substitutes (Univ. Cincinnati/Shriners Hospitals Collagen-based polymer with autologous fibroblasts Autologous keratinocytes Bum wounds congenital nevi chronic wounds (using allogeneic cells) [25-29]... 

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