Adsorption surface plasmon resonance

Fourier transform infrared spectroscopy Micro-flow imaging Poly(dimethylsiloxane) Polymethylsilsesquioxane Quartz crystal microbalance Random sequential adsorption Surface plasmon resonance Total internal reflection fluorescence X-ray photoelectron spectroscopy... 

There are a few other surface-sensitive characterization techniques that also rely on the use of lasers. For instance surface-plasmon resonance (SPR) measurements have been used to follow changes in surface optical properties as a fiinction of time as the sample is modified by, for instance, adsorption processes [ ]. SPR has proven usefiil to image adsorption patterns on surfaces as well [59]. 

Uchida K, Otsuka H, Kaneko M, Kataoka K, Nagasaki Y (2005) A reactive poly(ethylene glycol) layer to achieve specific surface plasmon resonance sensing with a high S/N ratio the substantial role of a short underbrushed PEG layer in minimizing nonspecific adsorption. Anal Chem 77 1075-1080... 

Shumaker Parry, J. S. Campbell, C. T., Quantitative methods for spatially resolved adsorption/desorption measurements in real time by surface plasmon resonance microscopy, Anal. Chem. 2004, 76, 907 917... 

Other teehniques used for studying molecular interactions between polymers and mueus inelude ultracentrifugation, surface plasmon resonance, and electromagnetic transduction [4,34]. Ilium and co-workers [35] investigated the interaction of chitosan microspheres using turbidimetric measurements and adsorption studies of mucin to the mierospheres. 

Jordan, C. E. and Corn, R. M. (1997) Surface plasmon resonance imaging measurements of DNA hybridization adsorption and strepta-vidin/DNA multilayer formation at chemically modified gold surfaces. Anal. Chem. 69, 1449-1456. 

Green RJ, Davies MC, Roberts CJ et al (1999) Competitive protein adsorption as observed by surface plasmon resonance. Biomaterials 20(4) 385-391... 

Reversible attachment of nanostructures at molecular printboards was exemplified by the adsorption and desorption of CD-functionalized nanoparticles onto and from stimuli-responsive pre-adsorbed ferrocenyl-dendrimers at a CD SAM (Fig. 13.7).65 Electrochemical oxidation of the ferrocenyl endgroups was employed to induce desorption of the nanostructure from the CD SAM. An in situ adsorption and desorption of ferrocenyl dendrimers and CD-functionalized Au nanoparticles (d 3 nm) onto and from the molecular printboard was observed by a combination of surface plasmon resonance spectroscopy (SPR) and electrochemistry. Similar behavior was observed when larger CD-functionalized silica nanoparticles (d 60 nm) were desorbed from the surface with the aid of ultrasonication. 

The quantitative difference in adsorption behavior of linear polymers between alkaline and acidic conditions can be verified from surface plasmon resonance (SPR) results [111]. As seen in Fig. 6, the adsorption kinetics change along any profile of rapid saturation, gradual increase after rapid increase or gradual increase, depending on the type of linear polymers. The equilibrium of adsorption is reached faster for NaPGA and NaHA than for DNA. However, the film at equilibrium is thicker at acidic condition than at alkaline condition. The quantitative estimation of adsorption of DNA on dendrimer SAMs can be also carried out from the intensity increase of a UV-vis absorption band at 258.8 nm [110]. 

The adsorption curve of DNA-conjugated TA-polyallylamine in pH 8.0 equilibration buffer indicated that the surface plasmon resonance (SPR) response was only slightly reversed by washing with buffer (Fig. 6A, curve I). 

In the kinetic studies of the adsorption process, the mass transport of the analyte to the binding sites is an important parameter to account for. Several theoretical descriptions of the chromatographic process are proposed to overcome this difficulty. Many complementary experiments are now needed to ascertain the kinetic measurements. Similar problems are found in the applications of the surface plasmon resonance technology (SPR) for association rate constant measurements. In both techniques the adsorption studies are carried out in a flow system, on surfaces with immobilized ligands. The role of the external diffusion limitations in the analysis of SPR assays has often been mentioned, and the technique is yet considered as giving an estimate of the adsorption rate constant. It is thus important to correlate the SPR data with results obtained from independent experiments, such as those from chromatographic measurements. 

Hook, F., Kasemo, B., Nylander, T., Fant, C., Sott, K., and Elwing, H. (2001). Variations in coupled water, viscoelastic properties, and film thickness of a Mefp-1 protein film during adsorption and cross-linking A quartz crystal microbalance with dissipation monitoring, ellipsometry, and surface plasmon resonance study.y4na/. Chem., 13, 5796-5804. 

Kolomenskii, A. A., Gershon, P. D., Schuessler, H. A. (1997) Sensitivity and detection limit of concentration and adsorption measurements by laser-induced surface-plasmon resonance. Applied Optics 36 6539-6547. 

Chemosensory applications will normally take place in an environment of complex composition. Humidity and other varying ambient conditions are in sharp contrast to the well-defined environment most typically found in related applications of imprinted polymers. Moreover, the trend in sensor technology towards miniaturisation, with the aim of future nano-scale dimensions, is a primary reason for rising perturbation sensitivity, such as new interfering forces that can be neglected in the macro range. Chemical sensors can be influenced by numerous factors, such as electrostatic effects (ChemFETs) or non-specific adsorption (SAW, surface plasmon resonance). 

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