Surface plasmon resonance technologies

Alternative methods to MS for the analysis of protein phosphorylation would involve immunoaffinity techniques using antibodies specific for phosphorylated residues. Surface plasmon resonance technology has been recently used for the determination of active concentration of phosphotyrosine-containing proteins at picomolar levels.117... 

The piezo-electric effect of deformations of quartz under alternating current (at a frequency in the order of 10 MHz) is used by coating the crystal with a selectively binding substance, e. g. an antibody. When exposed to the antigen, an antibody-antigen complex will be formed on the surface and shift the resonance frequency of the crystal proportionally to the mass increment which is, in turn, proportional to the antigen concentration. A similar approach is used with surface acoustic wave detectors [142] or with the surface plasmon resonance technology (BIAcore, Pharmacia). 

In the kinetic studies of the adsorption process, the mass transport of the analyte to the binding sites is an important parameter to account for. Several theoretical descriptions of the chromatographic process are proposed to overcome this difficulty. Many complementary experiments are now needed to ascertain the kinetic measurements. Similar problems are found in the applications of the surface plasmon resonance technology (SPR) for association rate constant measurements. In both techniques the adsorption studies are carried out in a flow system, on surfaces with immobilized ligands. The role of the external diffusion limitations in the analysis of SPR assays has often been mentioned, and the technique is yet considered as giving an estimate of the adsorption rate constant. It is thus important to correlate the SPR data with results obtained from independent experiments, such as those from chromatographic measurements. 

Protease assay CMV protease activity was measured using the surface plasmon resonance technology of the BIAcore instrument (Pharmacia Biosensor). Briefly, this instrument was used to quantitate residual uncleaved biotinylated... 

Usui-Aoki K, Shimada K, Nagano M, Kawai M, Koga H (2005) A novel approach to protein expression proWfing using antibody microarrays combined with surface plasmon resonance technology. Proteomics 5 2396-2401... 

Perspicace S, Banner D, Benz J et al (2009) Fragment-based screening using surface plasmon resonance technology. J Biomol Screen 14 337-349... 

Huber W, Mueller F (2006) Biomolecular interaction analysis in drug discovery using surface plasmon resonance technology. Curr Pharm Design 12 3999 -021... 

O Shannessy DJ, O Donell KC, Martin J, Brigham-Burke M (1995) Detection and quantitation of hexa-His-tagged recombinant proteins on Western blots and by surface plasmon resonance technology. Anal Biochem 229 119-124... 

Boozer, C., Kim, G., Cong, S., Guan, H., Londergan, T. (2006). Looking towards label-free biomolecular interaction analysis in a highthroughput format a review of new surface plasmon resonance technologies. Curr Opin Biotechnol 17, 400-405. 

Most information on how the trans-sialidase reaction proceeds was obtained from binding studies with either active T cruzi TS or with inactive natural mutants using surface plasmon resonance technology and NMR spectroscopy. While, for example, sialyllactose was readily bound to the enzyme protein, the acceptor substrate lactose or other asialo glycoconjugates did not interact with the enzyme at all unless it had been pre-incubated with the sialylated donor substrate. All of these studies clearly show that sialic acid modulates the afiSnity for die asialo acceptor substrate. The association of the asialo receptor with the active site is an... 

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