Adsorbates, polysaccharides

Polysaccharides interfaced with water act as adsorbents on which surface accumulations of solute lower the interfacial tension. The polysaccharide-water interface is a dynamic site of competing forces. Water retains heat longer than most other solvents. The rate of accumulation of micromolecules and microions on the solid surface is directly proportional to their solution concentration and inversely proportional to temperature. As adsorbates, micromolecules and microions ordinarily adsorb to an equilibrium concentration in a monolayer (positive adsorption) process they desorb into the outer volume in a negative adsorption process. The adsorption-desorption response to temperature of macromolecules—including polysaccharides —is opposite that of micromolecules and microions. As adsorbate, polysaccharides undergo a nonequilibrium, multilayer accumulation of like macromolecules. 

Silica-based material provides mechanical stability and high efficiency, while adsorbed polysaccharide helices offer chiral selectivity to the wide range of enantiomeric analytes. 

Other Surfactants. Most polysaccharides do not adsorb onto apolar surfaces. But those that do adsorb can provide strong steric repulsion. See Section 10.2.2 for adsorbing polysaccharides. 

Non-adsorbing polymers generate attractive interactions and depletion attractions, thus causing the system to phase-separate into one polymer-depleted and one particle-depleted solution. Typical polymers that could cause this behaviour are large non-adsorbing polysaccharides, such as xanthan or starch. This effect is usually observed as an increased creaming or a coarsening of the system. 

The interactions between different polysaccharide additives (such as guar gum or starch) and the components in a variety of pulp suspensions (including whole pulp and washed pulp) have been described as a four-step process (Fig. 7) mixing of polysaccharides into the pulp suspension complexation with DCS adsorption of polysaccharides (free or complexed) onto fibres and fines material and, finally, association of fines to the adsorbed polysaccharides on the fibres [48]. According to the authors, this agrees with the maximum fines retention, drainage and paper strength observed in industrial applications. 

Separation of enantiomers by physical or chemical methods requires the use of a chiral material, reagent, or catalyst. Both natural materials, such as polysaccharides and proteins, and solids that have been synthetically modified to incorporate chiral structures have been developed for use in separation of enantiomers by HPLC. The use of a chiral stationary phase makes the interactions between the two enantiomers with the adsorbent nonidentical and thus establishes a different rate of elution through the column. The interactions typically include hydrogen bonding, dipolar interactions, and n-n interactions. These attractive interactions may be disturbed by steric repulsions, and frequently the basis of enantioselectivity is a better steric fit for one of the two enantiomers. ... 

Polysaccharide-based CSPs incorporate derivatives of cellulose and amylose adsorbed on silica gel. The selectivity of these CSPs depends upon the nature of the substituents introduced during the derivatization process. The secondary structure of the modified polysaccharide is believed to play a role in selectivity, but the chiral recognition mechanisms have not been fully elucidated [55]. 

In some cases, the anionic POs adsorbed on chitin have similar antigenic determinants, but the plants belonging to different families - and even members of the same family - could have polysaccharide-specific POs with different structures. Thus, the majority of investigated species had anionic chitin-specific peroxidises, and these isoforms from potato Solcmaceae) and horseradish Brassicaceae) formed lines of precipitation with antibodies to wheat chitin-bound PO but not to anionic isoPO (Maksimov et al., 2000). However, protein extracts from several plants of Brassicaceae, Cucurhitaceae and Fahaceae formed precipitate with both the chitin-specific and anionic PO of wheat (Fig. 3). It was foimd that the greatest homology showed in plants and formed precipitation lines with the anionic PO of wheat (Tab. 2). 

It would be easier to describe those classes of compounds not normally separated by RPLC than to catalogue the applications to which RPLC has been turned. Applications for reversed phase can be found in virtually every area of analysis and are reviewed regularly in the journal Analytical Chemistry. RPLC has not been in general use for the analysis of inorganic ions, which are readily separated by ion exchange chromatography polysaccharides, which tend to be too hydrophilic to separate by RPLC polynucleotides, which tend to adsorb irreversibly to the reversed phase packing and compounds which are so hydrophobic that reversed phase offers little selectivity. 

Attachment There is a high specificity in the interaction between virus and host. The most common basis for host specificity involves the attachment process. The virus particle itself has one or more proteins on the outside which interact with specific cell surface components called receptors. The receptors on the cell surface are normal surface components of the host, such as proteins, polysaccharides, or lipoprotein-polysaccharide complexes, to which the virus particle attaches. In the absence of the receptor site, the virus cannot adsorb, and hence cannot infect. If the receptor site is altered, the host may become resistant to virus infection. However, mutants of the virus can also arise which are able to adsorb to resistant hosts. 

Fig. 3.5 Representation of a scheme of an experiment (upper set of drawings) and the obtained experimental results presented as AFM images (middle part) and cross-sectional profiles (bottom) that provides evidence of silica nucleation and shell formation on biopolymer macromolecules. Scheme of experiment. This includes the following main steps. 1. Protection of the mica surface against silica precipitation. It was covered with a fatty (ara-chidic) acid monolayer transferred from a water substrate with the Langmuir-Blodgett technique. This made the mica surface hydrophobic because of the orientation of the acid molecules with their hydrocarbon chains pointing outwards. 2. Adsorption of carbohydrate macromolecules. Hydrophobically modified cationic hydroxyethylcellulose was adsorbed from an aqueous solution. Hydrocarbon chains of polysaccharide served as anchors to fix the biomacromolecules firmly onto the acid monolayer. 3. Surface treatment by silica precursor. The mica covered with an acid mono-...

Biopolymers e.g., polysaccharides, polynucleotides, unfolded protein molecules, that all attain expanded flexible structures in solution adsorb more or less according to the principles discussed above. 

Several anionic carbohydrate polymers (e.g., carboxymethyl cellulose, xanthomonas campestris polysaccharide, cellulose sulfate ester, etc.) do not adsorb from fresh water solutions, but their adsorption in saline solutions plays an... 

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