Adenovirus production cycle

The adenovirus productive cycle has most usually been studied following infection of established lines of human cells, such as HeLa, with sufficiently high multiplicities of a human serotype to achieve a... 

The antiviral activity of a triterpene saponin isolated from angallis arvensis, was studied in vitro against several vimses including HSV-1, adenovirus type 6, vaccinia, vesicular stomatitis and poliovirus (Amoros et al. 1987). The drug was found to inhibit the replication of HSV-1 and poliovirus type 2 as shown by inhibition of cytopathic effect and reduction of virus production. The action was not due to a virucidal effect but might involve inhibition of virus-host cell attachment. Single cycle experiments indicated that saponins interfered with both early and late events of herpes virus replication (Amoros et al. 1987). 

Indeed, the whole question of the variable responses of mammalian cells to different human adenovirus serotypes is poorly understood, despite the fascinating range of interactions displayed. The variable abilities of cells derived from different species, or different tissues, to support adenovirus replication is generally interpreted in terms of the presence, or lack of, cellular factors that the virus needs to complete various steps in the replication cycle. More precisely, putative factors that permit human adenovirus replication in human cells may be present in, say, murine cells, but sufficiently divergent that they fail to interact optimally with the relevant viral components. Thus, an understanding in molecular terms of the steps in the viral replication cycle that are blocked in non- or semipermissive cells should provide important information about the host cell components utilized by the virus and, thus, the molecular interactions among viral and cellular products. 

The relevance of the examples of activated cellular gene expression that have been reported so far to the life cycle of the virus has also to be established. Adenovirus depends on its hose cell for a large number of products, whose synthesis might be expected to be enhanced soon after infection to prime the cell for the period when massive quantities of viral DNA, late mRNA, and structural proteins are made. At this juncture, it is, however, difficult to appreciate the significance of increased expression of such genes as those encoding P-tubulin and the 70K heat-shock protein. This may reflect our ignorance about many aspects of the virus-cell interaction. Alterna-... 

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