A-Hydroxymethyl acrylamide

HYDROXY-3-METHYL-4-ACETYLTETRAHYDROFURANE see BMK290 N-(HYDROXYMETHYL)ACRYLAMIDE see HLU500 a-HYDROXY-P-METHYL AMINE PROPYLBENZENE see EAWOOO... 

Treatment of a styrene-acrylamide latex similar to the one mentioned immediately above with 0.34 gm of 35% formaldehyde at various pH s for 1 hr at 50 C introduced A/-hydroxymethyl groups on the amido-nitrogens. The highest surface density of 7V-hydroxymethyl groups was obtained when the reaction was mn at pH 12.1. The product work-up was similar to that described above. 

Besides the most typical PNIPAAm in the family of poly(A-substituted acrylamide), there are many other polymers (Fig. 9.4) that show varied transition properties in response to temperature changes. For example, poly(A, Af -diethyl acrylamide) (PDEAAm) has an LCST in the range of 25-32 °C [4], poly[A-(D,L)-(l-hydroxymethyl) propylmethacrylamide] (PDL-HMPMA) has one close to 35 °C... 

Dissolve the CNBr fragments in standard SDS sample buffer (if the bromphenol blue in the sample buffer turns yellow, this indicates the presence of residual formic acid in the sample and small amounts of concentrated Tris base, pH 9, can be added to raise the pH). Analyze the peptides by SDS-PAGE on a 24% acrylamide, 0.054% bisacrylamide gel with a Tricine cathode buffer [0.1 M N-tris[hydroxymethyl]methylglycine (Tricine), 0.1% SDS, 0.1 M Tris base,... 

Hydroxyethyl acrylate Hydroxyethyl methacrylate Hydroxypropyl acrylate Hydroxypropyl methacrylate A -Methylol acrylamide A -Methylol methacrylamide Hydroxymethylated diacetone acrylamide Epoxy... 

Figure 7.10 Poly (A -substituted acrylamide) showing a LCST (a) Poly (iV-isopro-pylaciylamide) (PNIPAAm) (b) Poly(AyV-diethylacrylamide) (PDEAAm) (c) Poly(2-carboxyisopropylacrylamide) (PCIPAAm) (d) Poly(A -(Z) (l-hydroxymethyl), pro-pylmethacrylamide (P(Z-HMPMAAm)) (e) Poly(A - acryloyl-A/ -alkylpiperazine) [124]...

Acrylic acid esterified with cross-linked hydroxymethyl polystyrene or Wang resin reacts smoothly with primary or secondary aliphatic amines at room temperature (Entries 1 and 2, Table 10.6). Only sterically demanding amines or amines of low nucleophilicity (anilines, a-amino acid esters) fail to add to polystyrene-bound acrylate. Support-bound acrylamides are less reactive than acrylic esters, and generally require heating to undergo addition with amines (Entries 4 and 5, Table 10.6). a, 3-Unsaturated esters with substituents in the 3-position (e.g. crotonates, Entry 3, Table 10.6) react significantly more slowly with nucleophiles than do acrylates. The examples in Table 10.6 also show that polystyrene-bound esters are rather stable towards aminolysis, and provide for robust attachment even in the presence of high concentrations of amines. Entry 10 in Table 10.6 is an example of the alkylation of a resin-bound amine with an electron-poor alkene to yield a fluorinated peptide mimetic. 

HEA = Af-(Hydroxymethyl)methacrylamide TS = Af-[Tris(hydroxymethyl)methyl]acrylamide AA = Acrylic acid DMA = A,A-Dimethylacrylamide... 

Highly porous and hydrophilic supports have been prepared by co-polymerization of A-tris(hydroxymethyl)methyl acrylamide or A-tris(hydroxymethyl)-methyl methacrylamide with acrylic monomers bearing carboxyl functions, and then treated with 4-aminophenyl a-L-fucoside using a water-soluble carbodi-imide mediator. The support was used for purification of the L-fucose-binding lectin from Ulex europeus. 

Reaction between a water solution of formaldehyde with urea, melamine, and similar molecules (such as acrylamide) leads to hydroxymethylation of the nitrogens... 

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