3H-thymidine incorporation

Obermuller-Jevic et al. (2003) comparison PrEC-non-neoplastic cells M -trans/5% cis isomers 0.5—5.0pM THF affected cell cycle, corrected for THF alone values 48 h medium changed every 24 h Lycopene uptake 3H thymidine incorporation for proliferation... 

To establish whether rifaximin, like the other members of the rifamycin family [36, 58], specifically inhibits bacterial RNA synthesis the effect of this antibiotic as well as that of rifampicin and chloramphenicol on RNA (via 3H-uridine incorporation), DNA (via 3H-thymidine incorporation) and protein (via 35S-methionine incorporation) synthesis was studied in growing cultures of Escherichia coli [59], While chloramphenicol reduced protein synthesis, both rifaximin and rifampicin inhibited RNA synthesis in a concentration-dependent fashion. In contrast, none of them affected 3H-thymidine incorporation into DNA. These data suggest that rifaximin, like rifampicin, inhibits RNA synthesis by binding the (3 subunit of the bacterial DNA-dependent RNA polymerase [60],... 

Mehendale HM, Purushotham KR, Lockard VG. 1989. The time course of liver injury and [3H]thymidine incorporation in chlordecone-potentiated trichloromethane hepatotoxicity. Exp Mol Pathol 51 (1 ) 31 - 47. 

Ahmed, S.A., Gogal, R.M. Jr, and Walsh, J.E., A new rapid and simple non-radioactive assay to monitor and determine the proliferation of lymphocytes an alternative to (3H)thymidine incorporation assay, /. Immunol. Methods, 170, 211-224, 1994. 

Antiproliferation activity shown by the inhibition of [3H]thymidine incorporation into DNA in the proliferation of rat vascular smooth muscle cells. The effect was expressed as % of control 45 0-7.8,46 0-0.4. kActivity found as a constituent of a plant other than a Broussonetia species. 

In an effort to investigate antioxidant constituents with antiproliferative effects in rat vascular smooth muscle cells (VSMC), broussoflavan A (36) [49], broussoflavonols F (45) [50] and G (46) [51], and broussoaurone A (48) [49] were found to inhibit the Fe2+-induced thiobarbituric acid-reactive substance formation in rat brain homogenate. Furthermore, broussoflavonols F (45) and G (46) inhibited fetal calf serum-, 5-hydroxytryptamine-, or ADP-induced [3H]thymidine incorporation into rat VSMC [45]. Antioxidant activities and inhibitory effects on proliferation of rat VSMC with potent antiplatelet activities of 45 and 46 may be useful for vascular diseases and atherosclerosis [43,45]. 

In Swiss 3T3 cells, GRP stimulates [3H]thymidine incorporation in a concentration-dependent manner. Kuwanon H (2) inhibited GRP-induced DNA synthesis in Swiss 3T3 cells. The IC50 value was around 100 nmol/L, close to its AT, value for [125I]GRP binding to Swiss 3T3 cells, Fig. (19). Kuwanon H (2) demonstrated selectivity toward GRP, as concentration of KT6 mol/L uninfluenced basal and 5% serum-induced [3H]thymidine incorporation. From above results, kuwanon H (2) appears to be a selective antagonist for GRP-R. 

Figure 8. Effect of adding butyrate at different points in the cell cycle of mitotically selected KB cells on [3H]-thymidine incorporation.

Effects of Preincubating PBMC With Gangliosides for 18 Hours on 3H-Thymidine Incorporation (CPMxlO- ) Into PBMC8 ... 

Blair HC, Finch JL, Avioli R, et al. 1989. Micromolar aluminum levels reduce 3H-thymidine incorporation by cell line UMR 106-01. Kidney Int 35 1119-1125. 

Fig. 12.7. Time course of [3H]thymidine incorporation into DNA of UV irradiated, hydroxyurea-treated lymphocytes. 3 X106 lymphocytes from actinic keratosis patients ( ) or age-matched normal individuals (o) were incubated with [3H]thymidine (5/iCi/ml, 18.5 Ci/mmol) and hydroxyurea (1.5 mM) at 37°C immediately after irradiation (20 J-m 2). After incubating for different times cells were fixed in Camoy s fixative and washed with 5% trichloracetic acid and ethanol before counting. (Reproduced from Abo-Darub et al., 1978, with kind permission of the authors and...

Assess the cytotoxicity of the immunotoxin conjugate by [3H]thymidine incorporation assay. Pulse cells after 56 hby the addition of 0.5 iCi/well of [3H]thymidine and incubate for another 16 h. 

Coveney, M. F., and Wetzel, R. G. (1988). Experimental evaluation of conversion factors for the [3H] Thymidine incorporation assay of bacterial secondary production. Appl. Environ. Microbiol. 54, 2018-2026. 

A6. Ashton, I. K., and Pocock, A. E., Action of multiplication-stimulating activity on [3H]thymidine incorporation in rabbit and human fetal chondrocytes in vitro. J. Endocrinol. 99, 93-98 (1983). 

MLR. Human PBMCs were stimulated with inactivated PBMCs from an unrelated donor and cultured in the presence of increasing doses of calcitriol or ZK 191784. On day 5 cultures were labeled with [methyl-3H]thymidine for 6-h [3H] thymidine incorporation measured by liquid scintillation counts. (Adapted from Ref. [8] with permission from Macmillan Publishers.)... 

In vitro testing of delayed-type chemical hypersensitivity is based on the detection of chemical-specific IgG antibodies and/or T cells. Chemical-specific IgG antibodies are detected in solid-phase assays where the chemical is bound to various carriers, such as nitrocellulose or sepharose. These methods are controversial and are not recommended for the routine diagnosis of chemical hypersensitivity. Chemical-specific T cell responses are measured in the so-called LTT. This test reveals a sensitization of T cells by an enhanced proliferative response of peripheral blood mononuclear cells to a certain chemical. Generally, lymphocyte transformation is measured by [3H]thymidine incorporation into DNA, but alternative,... 

Wagner U, Burkhardt E, Failing K. Evaluation of canine lymphocyte proliferation comparison of three different colorimetric methods with the 3H-thymidine incorporation assay. Vet Immunol Immunopathol 1999 70 151-159. 

Carp oil ( Xg/mL) [3H]Thymidine incorporation into the DNA of LLC cells (x 103 dpm/well) Capillary-like tube formalion (mm length/field) Adherence of LLC cells to HMVEC (xlO3 LLC cells/well)... 

Scheving, L. E., T. H. Tsai, E. W. Powell, J. N. Pasley, F. Halberg, and J. Dunn. 1983. Bilateral lesions of suprachiasmatic nuclei affect circadian rhythms in [3H]-thymidine incorporation into deoxyribonucleic acid in mouse intestinal tract, mitotic index of corneal eptithelium and serum corticosterone. Anatomical Record 205 239-249. 

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