Zidovudine

Molecular formula C,oHi3Ns04 Molecular weight 267.2 CAS Registry No. 30516-87-1 

Sample preparation Condition a Baker CIS SPE cartridge with three 1 mL portions of MeOH, three 1 mL portions of water, and three 1 mL portions of buffer. Add a 200 p,L aliquot of plasma, bile, or liver homogenate containing the IS to 200 pL buffer and add this mixture to the SPE cartridge, wash with two 200 pL portions of buffer, allow cartridge to dry, elute with two 100 pL portions of MeOH, combine the eluates, add an equal volume of water, iiyect a 40 pL aliquot. (Buffer was 200 mM pH 7.5 sodium phosphate containing 8 mM tetrabutylammonium sulfate.) 

Mobile phase MeCN 200 mM pH 7.5 sodium phosphate + 8 mM tetrabutylammonium sulfate 5 95 (Wash column overnight with MeOH.) 

Molema, G. Jansen, R.W. Visser, J. Meijer, D.K.F. Simultaneous analysis of azidothymidine and its mono-, di- and triphosphate derivatives in biological fluids, tissue and cultured cells by a rapid high-performance liquid chromatographic method. J.Chromatogr., 1992, 579, 107-114 

Mobile phase Gradient. A was 1.44 mL concentrated phosphoric acid and 4 mL n-octylam-ine in 1 L water, pH adjusted to 6.55 with concentrated ammonia. B was MeCN. A B from 95 5 to 70 30 over 7 min, to 20 80 over 1.5 min, return to initial conditions over 1 min, re-equilibrate for 2.5 min. 

Sample preparation Condition a 3 mL 200 mg Bond Elut CIS SPE cartridge with 3 mL MeOH and 2 mL buffer, do not allow to go dry. 1 mL Plasma -I- 25 p.L 40 p.g/mL 7-ethyltheophylline in water, vortex, add to the SPE cartridge at not more than 0.5 mL/min, wash with 1 mL buffer, air dry for 3 min, elute with 2 mL MeOH. Evaporate the eluate to dryness under a stream of nitrogen at 50°, reconstitute the residue in 100 p,L MeCN 5% acetic acid 10 90, vortex vigorously, centrifuge at 10000 g for 5 min, inject a 20 xL aliquot of the supernatant. (Prepare buffer by diluting 1.44 mL concentrated phosphoric acid to 1 L with water and adjusting the pH to 6.55 with concentrated ammonia.) 

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Z-furan [710-25-8] Zidovudine [30516-87-1] Ziegler catalyst Ziegler catalysts... 

Adverse reactions associated with zidovudine include headache, weakness, malaise, nausea, abdominal pain, and diarrhea. Hematologic changes include anemia and granulocytopenia (low levels of granulocytes, atype of white blood cell, in the blood). 

ZIDOVUDINE The nurse assesses the patient for an increase in severity of symptoms of HIV and for symptoms... 

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