Yeasts cultivation media

B. Horstkotte, E. Werner, A.K. Seresht, G. Cornelissen, O. Elsholz, V. Cerda, R. Luttmann, Sequential injection analyzer for glycerol monitoring in yeast cultivation medium, Talanta 71 (2007) 941. 

Candida boidinii is a further yeast producer of pectic enzymes complex. The production is induced by the presence of pectin as a C-source in the medium the primary methabolic path is the utilization of methanol and the secondary the utilization of pectate chains. The pectic enzymes were bound on the cell walls or released on the cultivation medium. The main enzyme of pectic complex, polygalacturonase, was briefly characterized and the possibility to influence the production of its multiple forms discussed. 

The poor activities of pectic enzymes in the cultivation medium led us to prove the cell cytosole and the cell walls for these activities. The cytosole contains only traces of polygalacturonase activity, but the suspension of cell walls established the activity which seems to be widely sufficient for yeast growth and development. The characterization of this cell wall bound enzymes will be the object of our next studies. 

Yeast acid phosphatase (APase) Asymmetrical Separation of APase in cultivation medium identification of APase peak by enzymatic activity measurements [7]... 

The cultivation medium composition and the proteins excreted by the yeast cells were determined and their influence on the flotation performance was investigated by Bahr et al. [115]. The sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAAGE) and native PAAGE measurements indicated that during the cultivation various glycoproteins were excreted, which were distributed differently between the foam and the flotation residue liquid. A... 

Temperatures around 30 C have been utilized for xylitol production yeasts cultivated in synthetic medium or in hemicellulosic hydrolysates produced from... 

For small-scale cultivation of S. insignis, our laboratory has observed moderate anrhracycline production in GYE (glucose-yeast extract) medium consisting of 6.25% cerelose, 1.25% yeast extract, 0.33% NaO, and 0.625% GaCOj, pH 7.2 (78). Additional media useful for cultivation and fermentation of S. imignis also have been described pre-viousty (77,78). 

A. Bzducha-Wrobel, M. Kieliszek, S. Blazejak, Chemical composition of the cell wall of probiotic and brewers yeast in response to cultivation medium with glycerol as a carbon source, Eur. Food Re. Technol., 237,489-499, 2013. 

A. tubingensis strain NW756 was cultivated for 55 h at 30 C in minimal medium according to Pontecorvo et al [1] supplemented with yeast extract (2g/l) and 10 g/1 endo-PG II digested polygalacturonic acid (PGA). Complete hydrolysis of PGA by endo-PG II resulted in a mixture of mono, di and trigalacturonate [2]. Four subsequent colunm chromatographic steps were used for purification of the enzyme for which the data are summarized in Table 1. 

The cultivation of this yeast strain on pectin medium showed optimal grow conditions. The behaviour of this strain was compared with that of four strains of Candida boidinii from the Culture Collection of Yeasts. The grow curves of all strains on pectin medium showed marked plateau suggesting the presence of two existing C-sources in the pectin medium, requiring two different metabolic paths (Fig. 1). 

Pseudomonas spp. DSM 6611 and 6978 and Rhodococcus ruber DSM 44541 were cultivated in shaking flasks for 3 days at 30 °C with shaking at 120 rpm in YPG medium containing 10 g yeast extract, 10 g bacteriological peptone, 10 g glucose. 

Malt extract (1 g), yeast extract (1 g) and magnesium sulfate heptahydrate (0.012 g) were dissolved with water and the volume was adjusted to 100 mL with tap water. The culture medium (30 mL) was placed in a 500 mL shaking-flask with a cotton plug and sterilized (121 °C, 20 min). The seed culture broth was transferred to a 500 mL shaking-flask containing 30 mL of the culture medium. Cultivation was carried out for 48 h at 28 °C and 115 strokes per minute. 

Production of Lignin Peroxidase. Medium for the inoculum was rich in yeast extract (25 g/1) and glucose (25 g/l) to promote maximal growth of the mycelia. The inoculum of Phanerochaete chrysosporium ATCC 24725 was first cultivated for 3 days at 30°C in five litres of medium divided in five shake flasks. The shake flask batches were transferred to a 100 litre bioreactor and cultivated again for 3 days at 30°C. The batches were stirred and aerated to obtain maximal growth of mycelia. 

Production. The inoculum grew vigorously in the rich yeast extract containing media and produced a thick viscous dispersion in the stirred tank bioreactor. No lignin peroxidase activity could be detected at this stage. When transferred to the 1000 1 production bioreactor, the mycelium of P.chrysosporium attached completely to the nylon wool sheets within a few hours after inoculation and the medium remained completely clear throughout the cultivation. The enzyme had to be harvested immediately after the maximal activity level was reached due to its... 

You are going to cultivate yeast, Saccharomyces cerevisiae, by using a 10 m -fermenter your company already owns. You want to find out the amount of ethanol the fermenter can produce. Therefore, a chemostat study was carried out and the Monod kinetic parameters for the microorganism grown in the glucose medium at 30°C, pH 4.8, were found to be Ks = 0.0025 g/L and /imax = 0.25 h-1. The ethanol yield (YP/S) is 0.44 (g/g) and cell yield (Yx/S) is 0.019 (g/g). The inlet substrate concentration is 50 g/L-... 

Expression of eukaryotic genes in yeast has two main advantages (i) the yeast expression system contains many features of a eukaryotic expression system such as glycosylation or disulfide bond formation, and (ii) yeast is a very economical system. Yeasts are single cells, can be cultivated easily, feature fairly short doubling times, and require relatively inexpensive medium ingredients in many aspects they resemble bacteria. 

E. coli XLl-Blue was grown at 37°C in Luria-Bertani (LB) medium (containing 10 g/L of tryptone, 5 g/L of yeast extract, and 5 g/L of NaCl). Recombinant E. coli strains for the PHA production were cultivated at 30°C for 72 h in LB medium containing two different carbon sources (1) 2 g/L of sodium decanoate (Sigma, St. Louis, MO) and (2) 10 g/L of sodium gluconate (Junsei, Tokyo, Japan) plus 2 g/L of sodium decanoate. All the flask cultures were carried out in triplicate in a rotary shaker at 250 rpm. For the cultivation of recombinant E. coli strains, ampicillin (50 mg/L) was added to the medium. 

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