Yeasts identification

Hammer E, D Krpowas, A Schafer, M Specht, W Erancke, E Schauer (1998) Isolation and characterization of a dibenzofuran-degrading yeast identification of oxidation and ring clavage products. Appl Environ Microbiol 64 2215-2219. 

Zachaeiae, W. et al. Mass spec-trometric analysis of the anaphase-promoting complex from yeast identification of a subunit related... 

Pincus DH Rapid identification of Candida dubliniensis with commercial yeast identification systems. J Clin Microbiol 1999 37 3533-3539. 

Thelander, M, Fredriksson, D, Schouten, J, Hoge, JH, and Ronne, H, Cloning by pathway activation in yeast identification of an arabidopsis thaliana f-box protein that can turn on glucose repression. Plant Mol Biol, 2002. 49(1) 69-79. 

Several methods have been developed for the differentiation of yeasts. Traditional platting techniques may be adapted using selective and/or differential platting media. Such media have been developed for Zygosaccharomyces bailii, the most important of all food spoilage yeasts. However, yeast differentiation by differential media is poorly developed when compared with similar works applied to bacteria. Other phenotyping methods include conventional yeast identification by means of assimilation or fermentation tests and the use of morphological characteristics. Conventional methods are not suited to industrial laboratories even when these procedures are automated and computerized. 

Some promising new methods are not provided in this table however, they are discussed in Section 5.2.4 below. The variety of identification and differentiation methods is extensive only a few are implemented in the routine analysis conducted in commercial laboratories, and even fewer in the microbiology laboratories of breweries. A list of some of the established and reliable routine methods for rapid yeast identification is found in Section 5.2.3. Which method is applied in brewery laboratories or combined with the established routine methods depends on the practicability and the degree of aceeptance for new techniques, which require special training and knowledge of yeast handling. 

Table 5.2 Overview of methods for yeast identification and differentiation that can be applied for yeast species from a brewing environment... 

Brandi, A., Hutzler, M., Geiger, E. (2005). Optimisation of brewing yeast differentiation and wild yeast identification by real-time PCR. In Proc. 30th ebc congr. Prague. Fachverlag hans car/([CD-ROM], Ntimberg). 

Hutzler, M., Wenning, M. (2009). Yeast identification using infrared spectroscopy, friend Oder foe Brauwelt, 27,14-11. 

Further, microscopic observation is complicated by the fact that molds easily fragment when disturbed. Thus, preparation of wet mounts, as used in bacterial and yeast identification, is of minimal value. 

Lohmann C, Sabou M, Moussaoui W, Prevost G, Delarbre JM, Candolfi E, Gravet A, Letscher-Bru V. Comparison between the Biflex III-Biotyper and the Axima-SARAMIS systems for yeast identification by matrix-assisted laser desorption ionization-time of flight mass speetrometry. J Clin Microbiol. 2013 51(4) 1231-6. 

Dlauchy, D., j. Tornai-Lehoczli, and G. Peter. 1999. Restriction enzyme analysis of PGR amplified rDNA as a taxonomic tool in yeast identification. Sys. Appl. Microbiol. 22 445-453. 

A.-M. Freydiere, R. Guinet and P. Boiron, Yeast identification in the clinical microbiology laboratory phenotypical methods, Med. MycoL, 2001, 39, 9-33. 

Barnett, J.A., Payne, R.W. and Yarrow, D. (2000b) Yeasts Identification PC Program. Version 5, Barnett, Norwich, UK. 

Chambon C, Ladeveze V, Servouse M, Blanchard L, Javelof C, Vladescu B, Karst F (1991) Sterol pathway in yeast. Identification and properties of mutant strains defective in mevalonate diphosphate decarboxylase and famesyl diphosphate synthetase. Lipids 26(8) 633-636. doi 10.1007/bf02536428... 

Byrd, J. C., A. L. Tarentino, F. Maley, P. H. Atkinson, and R. B. Trimble, Glycoprotein synthesis in yeast. Identification of Man8GlcNAc2 as an essential intermediate in oligosaccharide processing, J. Biol Chem., 1982. 257, 14657-14666. 

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