Waters Acquity particles

The presence of 10% water in the eluent minimized variations. Two different LC modes were used for the analysis of the extracts a smaller (50 x 2.1 mm) Atlantis C18 column (5 /um particle size) on a Shimadzu liquid chromatograph and a UPLC column (Acquity C18,1.7 fim particle size, 50 x 2.1 mm) on a Waters Acquity system. In the Shimadzu experiment, a gradient from 0.5 min... 

A Waters Acquity UPLC system with a cooling autosampler and column oven was used. The stationary phase was a Waters Acquity BEH C18 column (50 x 2.1 mm, 1.7 /.un particle size). The column was maintained at 40°C. The mobile phase consisted of water and acetonitrile, each containing 0.3% formic acid and was delivered at 0.35 mL/min in a gradient mode at 60% water from 0 to 1.5 min, linearly decreased to 10% water in 0.5 min, and then returned to 60% water. Sample vials were maintained at 4°C. 

Fig. 8.3 UPLC analysis of Cupi/cMm-lyoplrilizEd pericarp carotenoids. Carotenoids detected by absorption at 454 nm, following separation on a waters acquity C18 1.8 xm HSS particle, 2.1 x 100 mm column resolved with 10% isopropanol (v/v) (a) and 100% acetonitrile (b). The solvent profile included two linear phases (0-3 min at 75% (b) 3-11 min from 95 to 100%) flow rate of 0.75 mL/min. (a) Standards (each at 100 ppm) capsorubin (1.7 min), capsanthin (2.08 min), antherxanthm (2.69 min), zeaxanthin (2.97 min), f -cryptoxanthin (4.86 min), and P-carotene (8.15 min), (b) Valencia pericarp extract, (c) NuMex Sunset pericarp extract...

Figure 8 UPLC-QTOF-MS base peak ion chromatogram obtained for the combined methanol extracts from soybean and Medicago truncatula (CV Jemalong A17). Separations were achieved using a Waters Acquity UPLC 2.1 x 100 mm, BEH C18 column with 1.7 pm particles, a flow of 600 jj.I min-1, and a linear gradient of 0.1% acetic acid acetonitrile (5 95 to 30 70 over 30 min). Mass spectra were collected on a Waters QTOFMS Premier. (Data generated by David Huhman.) Reprinted from M. Bedair L. W. Sumner, Trends Analyt. Chem. 2008, 27 (3), 238-250, Copyright (2008), with permission from Elsevier.

Reversed-phase UPLC separation system was also used for the determination of four water-soluble B vitamins, including B5, B8, B9, and B12 in fortified infant foods using Waters Acquity UPLC BEH C,g column (100 x 2.1 mm i.d., 1.7 pm particle size), and a binary gradient, acetonitrile-water mobile phase [85]. 

Folic acid and 5-CH3-H4folate have also been accurately measured in fortified breads by the UPLC-MS/MS method [101]. Reversed-phase Waters Acquity HSS T3 colnmn (100 x 0.1 mm i.d., 1.8 j,m particle size) has been eluted with a gradient mobile phase of 0.1% (v/v) formic acid in Milli-Q water and acetonitrile, the mobile phase delivered at 200 pL/min, and the sample injection volume fixed at 20 pL. Under these chromatographic conditions, the total run time was 6 min. 

UPLC was performed under gradient conditions consisting of 0.1% of formic acid in water and acetonitrile. Ten microliter of samples were injected on a Waters Acquity HSS T3 column (150 x 2.1 mm i.d., 1.8 jm particle size), followed by tandem mass spectrometry detection. The instrument was operated in the ESI positive mode and the data were acquired in MRM mode. 

Figure 5.10 Impedance time plots of a steroid separation obtained with columns packed with fully porous sub-2 p,m particles (Grace Vision HT C18, Waters Acquity BEH C18, and Hypersil Gold C18) and with a column packed with 2.7 p,m shell particles (Halo C18). From Fekete, S., et al.

A commercial HPLC system and columns capable of performing ultra high-pressure LC were recendy introduced at PITTCON 2004 (ACQUITY Ultra Performance LC System by Waters). This HPLC system was designed to take full advantage of the potential of novel, sub-2-micron particles to give scientists chromatographic run times that are up to 9 times shorter than current fast HPLC systems, up to 2 times better peak capacity or resolution, and 3 times better routine sensitivity. 

FIGURE 5 (a) Peptide digest run on a 4.8-gm particle on a traditional HPLC system. Peak count is 70, and peak capacity is 143. (b) Peptide digest run on a 1.7-gm particle on the ACQUITY UPLC system. Peak count is 168, and peak capacity is 360. (Courtesy of Waters Corp.)... 

In con (ras( to all other applications referred to in this article, that make use of 3.0-5 pm particles, Kintz et al. chose an ACQUITY C18 column (Waters) of 50 mm length and 2.1 mm I.D. packed with 1.7 pm particles. They analysed scopolamine from hair samples by LC-ESI-MS/MS with a flow of 0.3 ml/min [56], The retention time of scopolamine was as short as 1.13 min and the LOQ was excellent at 0.2 pg/ mg hair. This LOQ was 25-times lower than that obtained with conventional 3.5 pm material as reported before by the same research group (Table 8) [57],... 

Figure 3.11. A partial list of popular silica-based columns including some recent new offerings. Columns based on high-purity silica are underlined. Columns packed with hybrid particles are in bold. Note that the ACQUITY (Waters) and Gemini (Phenomenex) are new second-generation hybrid columns.

In order to overcome the pressure limitations and the other challenges. Waters Corporation introduced Acquity UPLC , the first commercially available system that addresses the challenge of using elevated pressure and sub-2 pm particles, which makes it a particularly attractive and promising analysis tool [51]. The UPLC instrumentation is designed to deliver mobile phase at pressures up to 1034 bar (15,000 psi). The researchers were very active in this area for some time to manufacture columns that can withstand these rigorous pressures [52,53]. 

Using Acquity UPLC HSS T3 column (50 x 2.1 mm i.d., 1.8 pm particle size) reversed-phase chromatography, water, 10 mM ammonium formate, 0.1% ammonium formate, 10 mM ammonium acetate, and 10 mM formic acid were tested for the aqneons bnffer component of the mobile phase, while methanol and acetonitrile were examined as the organic constituent Best peak shape, resolntion, and signal-to-noise ratio was achieved using a shallow gradient of 10 mM ammoninm formate and acetonitrile with an initial ratio of 95 5. Optimal chromatographic separations occurred at a flow rate of 0.3 mL/min, and a column temperature of dO C. The mass spectrometer was operated via positive ion mode ESI, and the vitamins were... 

UPLC-MS/MS analysis 10 pL of each extract was injected onto a UPLC-ESI-MS/MS system. Chromatographic separations were carried out using the Acquity UPLC BEH (bridged ethyl hybrid) C18 column 2.1 x 100 mm, and 1.7-pm particle size. The column was eluted under gradient from 95% of eluent A and 5% of eluent B to 100% of eluent B over 10.0 min, at a flow rate of 0.3 mL min. Eluent A water/ formic acid (0.1%, v/v) eluent B acetonitrile/formic acid (0.1%, v/v). 

---