Waters Corporation

This chapter gives a summary of the size exclusion columns currently available from Waters Corporation. It includes a description of the principles used to select the column type as well as a discussion of the properties and use of different columns. A few application examples are included as well. This review is designed to give the user a good overview over the properties and capabilities provided by Styragel, Ultrahydrogel, and Protein-Pak columns. 

Waters, Styragel, and Ultrahydrogel are registered trademarks of Waters Corporation. Protein-Pak is a trademark of Waters Corporation. 

The TSK-GEL column is a trademark of Tosoh Haas Co. Ltd and the Waters Insulin HMWP column is a trademark of Waters Corporation. The author thanks the book s editor Chi-san Wu, the assigned peer reviewer, and the Eli Lilly and Company reviwers John Towns, Ralph Riggin, and James Kelley for their assistance, time, and professional comments. 

Detectors Model 410 differential refractive index (Waters Corporation) Model 100 differential viscometer (Viscotek Corporation) Dawn-F multiangle laser light-scattering photometer (Wyatt Technology)... 

Figure 7.29 LC-PB-MS retention time-mass plot of a polymer additive extract. Courtesy of F. Ploeg, Waters/Micromass, Etten-Leur. Reproduced by permission of Waters Corporation...

Waters Corporation has now introduced the MicrobeLynx , a MALDI-TOF MS system developed for fingerprint bacterial ID. The system and its applications are described thus ... 

The LCQ and LCQ DECA are products of Thermo Electron Corporation (San Jose, CA) the Q-TOF and MassLyx are products of Waters Corporation (Beverly, MA) the QSTAR XL is a product of Applied Biosystems (Foster City, CA) and Spectrum Mill and the MSD TRAP XCT is a product of Agilent Technologies (Palo Alto, CA). 

Life Sciences R D, Waters Corporation, Milford, MA 01757, USA Nathan S. Buchanan... 

Fig. 4.17. The Right Hand Rule (I thumb, B index finger, Fl middle finger) to determine the direction of the Lorentz Force (a) the current corresponds to the direction where positive charges move, i.e., the figure directly applies for positive ions, (b) A real magnet yoke without coils and flight tube. With kind permission of Thermo Electron (Bremen) GmbH, (left) and Waters Corporation, MS Technologies, Manchester, UK (right).

Fig. 4.56. Schematic of the Q-TOF Ultima with ESI ion source in MS/MS mode. The TOF analyzer has a double reflector for higher resolution. Courtesy of Waters Corporation, MS Technologies, Manchester, UK.

Fig. 5.13. Ion source housing of a Autospec magnetic sector instrament. The ion source can be accessed from several directions to allow for simultaneous connection to DIP/DEP, GC and reference inlet system. By courtesy of Waters Corporation, MS Technologies, Manchester, UK.

Fig. 10.9. MALDI targets (a) Bruker Scout26 target with some sample preparations using different matrices and (b) Micromass target suitable for 96 samples as delivered from standard well plates, (b) By courtesy of Waters Corporation, MS Technologies, Manchester.

O Gara et al. [44] from Waters Corporation reported the preparation of an embedded octyl carbamate phase (12% C) using a single-step synthesis in 1995, having the structure... 

Diode resolution is an important consideration in a PDAD detector. It is calculated by dividing the wavelength range by the number of diodes in the array. This, in combination with the optical resolution, will affect the spectral resolution of the instrument (i.e., the wavelength in nm, between data points in an acquired spectrum). (Operation and Maintenance of the ZQ with MassLynx, Waters Corporation.)... 

FIGURE 17 Retention map and chromatograms of two basic antidepressants using mobile phases at various pH illustrating the importance of pH in the separation of basic analytes. Figure reprinted with permission from Waters Corporation. 

Data extracted from published specifications by Waters Corporation in 2003. 

I would also like to thank Todd Miller, John Van Antwerp, and Jeff Jalinski of Waters Corporation, and Michael Dong of Purdue Pharma for their helpful ideas and review of the material included in this chapter. 

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