Acquity UPLC BEH

AT, FB, and their impurities Tablets Acquity UPLC BEH C18 column (1.7 urci, 2.1 mm x 100 mm) Gradient elution of acetonitrile ammonium acetate (pH 4.7 0.01 M) Minimum detection limit -was atng/1 levels [15]... 

Acquity UPLC BEH C,8 column Acquity UPLC BEH Shield C18 (150 X 2.1 mm,... 

Flow-rate 0.3 mL/min Column temperature 40°C Offline SPE Acquity UPLC BEH C,8 column... 

Acquity UPLC BEH C,5 column (50 X 2.1 mmi.d., 1.7 pm particle size) Gradient elution... 

Four columns and several mobile phases were compared in this study. Acquity UPLC BEH Shield RP,g (100 x 2.1 mm i.d., 1.7 pm particle size), Acquity UPLC BEH Ci8 (100 X 2.1 mm i.d., 1.7 pm particle size), Acquity UPLC Tg (100 x 2.1 mm i.d., 1.7 pm particle size), and YMC30 (150 x 4.6 mm i.d., 3 pm particle size) were used for comparisons to the separation capability in vitamin Kl isomers. The type of column plays an important role in the separation of vitamin Kl isomers. As a result. 

In preliminary tests, four ODS Qg columns with different colunm size and/or different particle size were compared regarding the separation of the four vitamins and methotrexate internal standard. Column A is a Waters Atlantis Cj column (150 X 2.1 mm i.d., 5 pm particle size), column B is a Waters Sunfire Cj column (150 X 2.1 mm i.d., 5 pm particle size), column C is a Waters Acquity UPLC BEH Cj column (50 x 2.1 mm i.d., 1.7 pm particle size), and column D is a Waters Acquity UPLC BEH C18 column (100 x 2.1 mm i.d., 1.7 pm particle size). 

Choosing a chromatographic column gives the possibility to perform various water-soluble vitamin and vitamin-like compound separations in one run and with better resolution. Chromatographic separation was performed using Waters Acquity UPLC BEH Shield RP,g column (100 x 2.1 mm i.d., 1.7 pm particle size) at a flow rate of 0.2 mL/min, and the injection volume was 10 pL. The mobile phase also has a significant effect on peak shape, sensitivity, and resolution. Methanol with 10 mM aqueous ammonium acetate gave the best overall performance and was deaned to be most suitable when all 14 compounds were considered. 

A Waters Acquity UPLC coupled to an Acquity tandem quadrupole detector (Waters Corporation, Manchester, UK) was used for all analyses. Chromatographic separations were performed on Acquity UPLC BEH C18 1.7 jm column (2.1 x 100 mm column) at 45°C at a flow rate of 400 aL/min running a gradient 98% of A as initial setting for... 

UPLC-MS/MS analysis 10 pL of each extract was injected onto a UPLC-ESI-MS/MS system. Chromatographic separations were carried out using the Acquity UPLC BEH (bridged ethyl hybrid) C18 column 2.1 x 100 mm, and 1.7-pm particle size. The column was eluted under gradient from 95% of eluent A and 5% of eluent B to 100% of eluent B over 10.0 min, at a flow rate of 0.3 mL min. Eluent A water/ formic acid (0.1%, v/v) eluent B acetonitrile/formic acid (0.1%, v/v). 

Column Acquity UPLC BEH C18 column (2.1mmID x 50 mm, 1.7 pm particles)... 

Stationary phase Acquity UPLC BEH C18 with Acquity BEH C18 pre-column. 

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