Optimization of chromatographic separations

The optimization of chromatographic separations can generally be seen as a compromise between speed, i.e., to produce the largest possible amount of data or substance per unit time, and resolution, i.e., to produce the highest possible quality of data or purity of substance. Obviously the goal for optimization differs according to the purpose of the separation and also between scale of operation. Therefore, different parameters are critical for different situations. Still, some basic rules for optimization may be applied. 

For optimization of chromatographic separations the ratio of the time spent by the solute in the stationary phase to the time it spends in the mobile phase is more fundiwentally i tortant. This ratio is called the solute capacity factor and is given by equation (1.8)... 

Other Examples of the Use of Principal Properties Characterization by principal properties has been reported for classes of compounds in applications other than organic synthesis Aminoacids, where principal properties have been used for quantitative structure-activity relations (QSAR) of peptides [64], Environmentally hazardous chemicals, for toxicity studies on homogeneous subgroups [65]. Eluents for chromatography, where principal properties of solvent mixtures have been used for optimization of chromatographic separations in HPLC and TLC [66],... 

The capacity factor plays an important role in optimization of chromatographic separations. It is related to the partition coefficient of the solute between the mobile phase and stationary phase K in the following way ... 

In addition, chemometricians contribute to the development of all these methods. As a rule, these developments are dedicated to particular practical requirements, such as the automatic optimization of chromatographic separations or in prediction of the biological activity of a chemical compound. 

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