Water Staphylococcus aureus growth

Correlation between lag phase (hours) and cell growth at 24 h (log) for Staphylococcus aureus and physico-chemical parameters measured for BHI (black circles) and BHliNaCl 1 1 mixture (white triangles) water activity, population of bound water (Pbw)/ NMR relaxation rate (Ri),... 

Lavoie, J., Labbe, R.G., and Chinachoti, P. Comparison of NMR water mobility to water activity for the determination of growth of Staphylococcus aureus, /. Food Sci, 62, 861,1997. 

Troller, J. A. (1972). Effect of water activity on enterotoxin A production and growth of Staphylococcus aureus. Appl. Microbiol. 24,440. 

Documented effects Alcohol and water extracts of the roots inhibited the growth of Aeromoms hydrophila. Bacillus mega-terium, Corynebacterium xenosis, Pseudomonas aeruginosa. Micrococcus luteus. Enterococcus faecalis, and Staphylococcus aureus, but was not an effective inhibitor of Escherichia coli (Golcu et al. 2002). In experiments with rats that ate fresh roots decreased bladder and kidney stone formation was observed, but increased death rates were exhibited. In experiments with rabbits that were given root extracts orally, decreased calcium oxalate crystal formation in the kidneys and hepatotoxicity was observed. Genotoxic effects were observed in bacterial and mammalian cell systems (Blumenthal 1998). 

Presumptive test The various types of media used for detecting Staphylococcus aureus are presented in Table 4.15. Water samples (100 mL) are filtered through a 0.45 xm membrane. The membrane is then placed on Baird-Parker medium and incubated at 35-37°C + 1°C for 24r-48 h. The medium contains pyruvate and glycine that selectively stimulate the growth of Staphylococcus spp. Colonies measuring 1-5 mm in diameter that are shining, black, convex, and surrounded by a clear zone suggest the presence of S. aureus [2]. 

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