Water column particulates

Normal-phase HPLC has also found application in the analysis of pigments in marine sediments and water-column particulate matter. Sediments were extracted twice with methanol and twice with dichloromethane. The combined extracts were washed with water, concentrated under vacuum and redissolved in acetone. Nomal-phase separation was performed with gradient elution solvents A and B being hexane-N,N-disopropylethylamine (99.5 0.5, v/v) and hexane-2-propanol (60 40, v/v), respectively. Gradient conditions were 100 per cent A, in 0 min 50 per cent A, in 10 min 0 per cent A in 15 min isocratic, 20 min. Preparative RP-HPLC was carried out in an ODS column (100 X 4.6 mm i.d. particle size 3 jum). Solvent A was methanol-aqueous 0.5 N ammonium acetate (75 25, v/v), solvent B methanol-acetone (20 80, v/v). The gradient was as follows 0 min, 60 per cent A 40 per cent A over 2 min 0 per cent A over 28 min isocratic, 30 min. The same column and mobile phase components were applied for the analytical separation of solutes. The chemical structure and retention time of the major pigments are compiled in Table 2.96. 

R. Goericke, A. Shankle and D.J. Repeta, Novel carotenol chlorin esters in marine sediments and water column particulate matter. Geochim. Cosmochim. Acta 63 (1999) 2825-2834. 

Shafer, M. M. Biogeochemistry and Cycling of Water Column Particulates in Southern Lake Michigan. Ph.D. Thesis, University of Wisconsin—Madison, Madison, WI, 1988. 

Estuarine water column particulates, primarily derived from rivers, adjacent wetland systems, and resuspension events, have been shown to be important in controlling the fate and transport of chemicals in estuaries. In particular, mineral surfaces on these particles have been shown to be important in binding organic molecules, gels, and microaggregates. 

Sample Collection, Pretreatment, and Analysis. Sediment-bound phosphorus in the Genesee River was studied by sampling bottom sediment, fine-material washed from bottom sediment, suspended sediment, and water column particulate material at six stations on the river. The sampling program was planned to be synoptic with complete chemical and hydrological parameters recorded at each site. One kilogram surficial sediment samples were collected in midstream at most sites during six field trips... 

Depth profiles of cell densities in the photic zone generally show E. huxleyi to live within the mixed layer. Cortes et al. (2001) studied the seasonal depth distribution of coccohthophorid species off Hawaii. Sampling showed that the main production occurred in the middle photic zone (50-100 m), which lay within the mixed layer for most of the year. While the depth of maximum E. huxleyi density varied during the annual cycle, it generally lay between the shallowest sampling level (10 m) and 100 m. Depth profiles off Bermuda (Haidar and Thierstein, 2001) found that maximum densities of E. huxleyi were nearly always shallower than 100 m, and more commonly within the upper 50 m. The highest cell densities for E. huxleyi recorded were at 1 m depth in March, after the seasonal advection of nitrate into the mixed layer. Seven years of water-column particulate data off Bermuda confirm that alkenone concentrations in the surface mixed layer are 2-4 times higher than in the deep fluorescence maximum at 75-110 m (Conte et al., 2001). 

Biphytanes derived from membrane ether lipids of archaea have been found in water column particulates, and sedimentary organic matter (Hoefs et al. 1997). 

Plutonium solutions that have a low activity (<3.7 x 10 Bq (1 mCi) or 10 mg of Pu) and that do not produce aerosols can be handled safely by a trained radiochemist in a laboratory fume hood with face velocity 125—150 linear feet per minute (38—45 m/min). Larger amounts of solutions, solutions that may produce aerosols, and plutonium compounds that are not air-sensitive are handled in glove boxes that ate maintained at a slight negative pressure, ca 0.1 kPa (0.001 atm, more precisely measured as 1.0—1.2 cm (0.35—0.50 in.) differential pressure on a water column) with respect to the surrounding laboratory pressure (176,179—181). This air is exhausted through high efficiency particulate (HEPA) filters. 

Relatively low operating pressure drops (for degree of particulate removal obtained) in the range of approximately 2- to 6-in water column... 

There are two main sources of Rn to the ocean (1) the decay of sediment-bound "Ra and (2) decay of dissolved "Ra in a water column. Radon can enter the sediment porewater through alpha recoil during decay events. Since radon is chemically inert, it readily diffuses from bottom sediments into overlying waters. The diffusion of radon from sediments to the water column gives rise to the disequilibrium (excess Rn) observed in near-bottom waters. Radon is also continuously being produced in the water column through the decay of dissolved or particulate "Ra. 

TBT exists in solution as a large univalent cation and forms a neutral complex with CH or OH . It is extremely surface active and so is readily adsorbed onto suspended particulate material. Such adsorption and deposition to the sediments limits its lifetime in the water column. Degradation, via photochemical reactions... 

The test divides the drilling fluid into three phases the liquid phase, the suspended particulate phase, and the solid phase. These phases are designed to represent the anticipated conditions that organisms would be exposed to when drilling mud is discharged into the ocean. Certain drilling fluid components are water column, others are fine particulates which would stay suspended, and still water soluble and will dissolve in the other material would settle rapidly to the bottom. 

Fig. 10-15 Organic carbon fluxes with depth in the water column normalized to mean annual primary production rates at the sites of sediment trap deployment. The undulating line indicates the base of the euphotic zone the horizontal error bars reflect variations in mean annual productivity as well as replicate flux measurements during the same season or over several seasons vertical error bars are depth ranges of several sediment trap deployments and uncertainities in the exact depth location. (Reproduced with permission from E. Suess (1980). Particulate organic carbon flux in the oceans - surface productivity and oxygen utilization, Nature 288 260-263, Macmillan Magazines.)...

As a starting point we can view the ocean as one large reservoir to which materials are continuously added and removed (Fig. 10-17). The major sources of material include rivers and winds, which carry dissolved and particulate materials from the continents to the sea. The major removal process is the formation of marine sediments both by settling of particles through the water column as well as by precipitation of insoluble solid phases. For many ele-... 

The water column distribution of particulate " Th in partially mixed estuaries aids in assessing the transport of particles throughout the system, as a consequence of tidal mixing or the estuarine circulation. Feng et al. (1999a) took advantage of the fact that the... 

Baskaran and Santschi (1993) examined " Th from six shallow Texas estuaries. They found dissolved residence times ranged from 0.08 to 4.9 days and the total residence time ranged from 0.9 and 7.8 days. They found the Th dissolved and total water column residence times were much shorter in the summer. This was attributed to the more energetic particle resuspension rates during the summer sampling. They also observed an inverse relation between distribution coefficients and particle concentrations, implying that kinetic factors control Th distribution. Baskaran et al. (1993) and Baskaran and Santschi (2002) showed that the residence time of colloidal and particulate " Th residence time in the coastal waters are considerably lower (1.4 days) than those in the surface waters in the shelf and open ocean (9.1 days) of the Western Arctic Ocean (Baskaran et al. 2003). Based on the mass concentrations of colloidal and particulate matter, it was concluded that only a small portion of the colloidal " Th actively participates in Arctic Th cycling (Baskaran et al. 2003). 

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