Vitamin minimum

For cats, add 10 lU vitamin E above minimum level per gram of fish oil per kilogram of diet. 

Vitamins A, D, and E are required by mminants and, therefore, their supplementation is sometimes necessary. Vitamin A [68-26-8] is important in maintaining proper vision, maintenance and growth of squamous epitheHal ceUs, and bone growth (23). Vitamin D [1406-16-2] is most important for maintaining proper calcium absorption from the small intestine. It also aids in mobilizing calcium from bones and in optimizing absorption of phosphoms from the small intestine (23). Supplementation of vitamins A and D at their minimum daily requirement is recommended because feedstuffs are highly variable in their content of these vitamins. 

The quaHty, ie, level of impurities, of the fats and oils used in the manufacture of soap is important in the production of commercial products. Fats and oils are isolated from various animal and vegetable sources and contain different intrinsic impurities. These impurities may include hydrolysis products of the triglyceride, eg, fatty acid and mono/diglycerides proteinaceous materials and particulate dirt, eg, bone meal and various vitamins, pigments, phosphatides, and sterols, ie, cholesterol and tocopherol as weU as less descript odor and color bodies. These impurities affect the physical properties such as odor and color of the fats and oils and can cause additional degradation of the fats and oils upon storage. For commercial soaps, it is desirable to keep these impurities at the absolute minimum for both storage stabiHty and finished product quaHty considerations. 

Ascorbic acid is very soluble in water and mainly excreted in the urine. No ascorbic acid is excreted during vitamin C deficiency. A minimum amount is lost in the feces, even after intake of gram dosages (154). 

Approximately 0.05 to 0.2% of vitamin > 2 stores are turned over daily, amounting to 0.5—8.0 )J.g, depending on the body pool size. The half-life of the body pool is estimated to be between 480 and 1360 days with a daily loss of vitamin > 2 of about 1 )J.g. Consequentiy, the daily minimum requirement for vitamin B22 is 1 fig. Three micrograms (3.0 J.g) vitamin B22 are excreted in the bile each day, but an efficient enterohepatic circulation salvages the vitamin from the bile and other intestinal secretions. This effective recycling of the vitamin contributes to the long half-life. Absence of the intrinsic factor intermpts the enterohepatic circulation. Vitamin > 2 is not catabolized by the body and is, therefore, excreted unchanged. About one-half of the vitamin is excreted in the urine and the other half in the bile. 

It is assumed that in order to have vitamin A activity a molecule must have essentially one-half of its structure similar to that of (i-carotene with an added molecule of water at the end of the lateral polyene chain. Thus, P-carotene is a potent provitamin A to which 100% activity is assigned. An unsubstituted p ring with a Cii polyene chain is the minimum requirement for vitamin A activity. y-Car-otene, a-carotene, P-cryptoxanthin, a-cryptoxanthin, and P-carotene-5,6-epoxide aU have single unsubstimted rings. Recently it has been shown that astaxanthin can be converted to zeaxanthin in trout if the fish has sufficient vitamin A. Vitiated astaxanthin was converted to retinol in strips of duodenum or inverted sacks of trout intestines. Astaxanthin, canthaxanthin, and zeaxanthin can be converted to vitamin A and A2 in guppies. ... 

CF patients with low bone mineral density and low serum vitamin D levels may improve bone health through supplemental vitamin D analogs beyond those found in standard CF vitamins. The optimal dose and analog have not been determined. For ergocalciferol, a minimum of 400 IU and 800 IU... 

Immediate administration of 100 meg to 1 mg vitamin K subcutaneously or intravenously, followed by administration of FFP as necessary. Vitamin K administration via the intramuscular route may induce hematoma owing to coagulopathy, and intravenous administration should be slow because it has been associated with anaphylaxis. Prothrombin complex concentrate (PCC) at minimum dose of 50 units/kg should be given for life-threatening bleeding.27,28... 

Only one analytical method has been widely applied to the measurement of vitamins in seawater. The method, bioassay, is not really within the realm of the analytical chemist, since it requires the maintenance of cultures of test organisms. The tests also usually require a minimum of four days before results are available. 

Paul and Paul35 made a study of the effect of various levels of vitamin A intake, particularly on the eye and tooth development of albino rats. They found that 10 times the supposed minimum gave the best response of any of the levels tested. At this level (20 units per 100 gm. body weight daily) 18 of the 19 animals were "normal," both as to teeth and eyes, but one animal was listed as slightly abnormal as to teeth, and one definitely so with respect to eyes. At 1/5 of this level (4 units per 100 gm.) 20 per cent were normal as to teeth and 60 per cent were normal as to eyes. At 1/10 of the top level (2 units per 100 gm.) 26 per cent were normal as to eyes but none were normal as to teeth. It is apparent from these data that the 20 unit level was not sufficient for some of the rats, but that the 4 unit level was sufficient for about 20 per cent. This indicates a greater than 5-fold spread even among these relatively homogeneous albino rats. 

A vitamin has been defined [399] as a biologically active organic compound that is essential for the organism s normal metabolic and physiologic functions. Vitamins are not produced by human body itself, thereby they have to be obtained from the diet. As micronutrients, in contrast to nonessential chemical substances, they present both a minimum and a maximum level of intake beyond which arise risks of deficiency conditions or adverse effects [400]. 

Thiamin 0.23 mg or more per 1000 kcal of food consumed and a minimum total of 0.8 mg/day. Replacement of the methyl group on the pyrimidine ring by ethyl, propyl, or isopropyl gives compounds with some vitamin activity, but replacement by hydrogen cuts activity to 5% of the original. The butyl analog is a competitive inhibitor. 

Honey. A natural syrup which vanes in composition and flavor, depending upon die plant source from which die nectar was collected by die honeybee, the amount of processing, and the duration of storage. The pnncipal sugars contained in honey are fructose and glucose, the same components as in table sugar. There are minute amounts of vitamins and minerals in honey, but these are not usually considered in terms of calculating minimum requirements. 

Retinol and its esters exhibit similar UV absorption spectra within a broad wavelength range and have practically equal molar absorptivities when dissolved in a given solvent. The e value of crystalline all-frans-retinol in 2-propanol at the Amax of 325 nm is 52,300 (120), which corresponds to an A m of approximately 1830. The on-column minimum detectable quantity of vitamin A using UV absorption is approximately 2 ng (121). 

It is fortuitous that indigenous concentrations of vitamin E in the principal food sources are on the order of mg rather than yug/100 g, for the tocopherols and tocotrienols exhibit relatively low intensities of UV absorption. Individual vitamers are characterized by a slightly different absorption maximum within the wavelength range of 292-298 nm in ethanol. Published A] m values for the tocopherols at their Amax in ethanol are a-T 70-73.7 at 292 nm, (3-T 86-87 at 297 nm, y-T 90-93 at 298 nm, and <5-T 91.2 at 298 nm (126). The different absorption characteristics among the vitamers necessitates the running of individual standards for accurate quantitation of each vitamer. The absorption intensity of a-tocopheryl acetate is lower still with an A j m value of only 40-44 at the Amax of 285.5 nm (44). Reported minimum detectable quantities of a-, / -, y-, and (5-tocopherols at 295 nm are, respectively, 50 ng, 70 ng, 90 ng, and 130 ng (127). 

Total Vitamin C extraction with 12.5% trichloroacetic acid reduce DHAA with 0.8% DL-homo-cysteine (minimum 40 1 excess of homocysteine DHAA). 

The desugaring of cane juice concentrates the heat- and alkali-stable vitamins in the final molasses. Even after this accumulation, only myo-inositol may have reached the level of minimum dietary requirements.109 Niacin, pantothenic acid and riboflavin are also present in significant quantities109 the thiamine, pyridoxin, pantothenic acid, biotin and folic acid contents of molasses have been estimated by bioassay.110 111 The biotin content of Hawaiian and Cuban molasses was 2.1 and 1.7 gammas per gram, respectively.119 The antistiffness factor (closely related to stigmasterol) has been found in cane molasses.88 89 The distillery slop from the yeast fermentation of molasses is marketed as a vitamin concentrate this product also contains vitamins originating in the yeast. 

Figure 1 is the ultraviolet spectrum of a 10 mcg/ml solution of vitamin D3 in methanol. The spectrum was obtained using a Cary Model 219 recording spectrophotometer (Varian Instrument Co., Palo Alto, CA). Vitamin D3 and related compounds have a characteristic UV absorption maximum at 265 nm and a minimum at 228 nm. The extinction coefficient at 265 nm is about 17,500 and 15,000 at 254 nm. An index of purity of vitamin D3 is a value of 1.8 for the ratio of the absorbance at 265 to that at 228 nm. The high absorbance at 254 nm enables one to use the most common and sensitive spectrophotometric detector used in high performance liquid chromatography (HPLC) for the analysis of vitamin D3 in multivitamin preparations, fortified milk, other food products, animal feed additives etc. 

The numbers in parentheses represent the minimum amounts (pg) of vitamin Dg that can be detected and identified on the plates. 

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