Virus-infected cells identification

Sawyer, S.T., Krantz, S.B., and Luna, J. (1987) Identification of the receptor for erythropoietin by cross-linking to Friend virus-infected erythroid cells. Proc. Natl. Acad. Sci. USA 84, 3690-3694. 

In 1961 it was reported that human leukocytes were capable of producing IFN in response to viral infections [8,9]. This viral stimulation of white blood cells was initially used to produce leukocyte IFN for clinical applications. Identification of a number of varied IFN inducers such as mycoplasma or other microorganisms in cell cultures, lipopolysaccharides (LPS, derived from bacteria membranes), tumor-derived or virus-transformed cells, and synthetic chemical compounds such as polyanions and poly I C (poly inosine-cytosine) suggested that different IFN mixtures could be derived from interaction of various inducing agents and appropriate target cells [10-16]. Another pH-labile, nonvirus-induced IFN termed immune-IFN (induced by immune effector cells) was discovered in 1965. It was produced by... 

Shang, F., Huang, H., Revesz, K., Chen, H.-C., Herz, R., and Pinter, A. (1991) Characterization of monoclonal antibodies against the human immunodeficiency virus matrix protein pl7 identification of epitopes exposed at the surfaces of infected cells. J. Virol. 65,4798-4804. 

Margulies BJ, Browne H, Gibson W. Identification of the human cytome ovirus G protein-coupled receptor homologue encoded by UL33 in infected cells and enveloped virus particles. Virology 1996 225(1) 111 25. 

One aspect of viral proteomics has been the characterization of virus particles and virally infected cells. Characterization of purified virions has led to the identification of viral proteins that were not originally identified with the virion as well as the identification of cellular proteins associated with the purified virus. For example, analysis of HCMV viral particles identified 12 additional ORFs not previously known to reside in virions as well as the identification of 71 cellular proteins [6]. The importance of these cellular and viral proteins in viral replication or pathogenesis awaits further analysis. Additionally, 12 unique polypeptides were identified that did not correspond to previously identified ORFs [6], illustrating the fact that despite intensive sequence analysis, sequence characteristics of viral promoters and ORFs are still not entirely understood. Analysis of virally infected cells has also led to the characterization of events leading to EBV-induced transformation [9,10,47], identification of cellular proteins induced in HIV-infected... 

Burgoyne, R. D., and Stephen, J., 1979, Further studies on a vaccinia virus cytotoxin present in infected cell extracts. Identification as surface tubule monomer and possible mode of action. Arch. Virol. 59 107. 

MHC Class I proteins identification of cells infected with a virus... 

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