Vibrio fisheri

Environment Canada (1992a) Biological test method toxicity test using luminescent bacteria (Vibrio fisheri), Environmental Protection Publications, Environment Canada, Ottawa, EPS Report l/RM/24,... 

The Microtox assay which measures light inhibition with the bacterium Vibrio fisheri is a well known and useful aquatic toxicity test (see Chapter 1, volume 1 of this book). As previously reported (Blaise et al., 1994) and based on our own experience, it appears more appropriate to determine 60 min IC50 for waste leachates, as opposed to 15 min or 30 min endpoints. IC50s measured after 60 min on MIOM leachates were clearly more sensitive and reproducible than those measured at 30 min and 15 min (Ferrari et ah, 1999). Since WASTOXHAS was applied on (poly)metallic matrices in this study, we also found it more suitable to use zinc sulphate as a reference toxicant to periodically verify the sensitivity of the Microtox bacterial light reagent. 

Microtox test) Vibrio fisheri ISO 11348-3 (1999) Inhibition of bioluminescence Acute... 

ISO 11348-3 (1999) Water quality - Determination of the inhibitory effect of water samples on the light emission of Vibrio fisheri (luminescent bacteria test) - Part 3. Method using freeze-dried bacteria, International Organization for Standardization, Geneva. 

A bacterial assay, the Photobacterium test, based on the inhibition of light emission of a bioluminescent bacterium Vibrio fisheri (ISO, 1998), originally developed to test the toxicity of industrial effluents, gives an indication of the effects of the test agent on the oxidative metabolism of the cell. As a bacterial assay its advantages are rapidity and relatively low costs, but naturally the differences between bacterial and mammalian systems make the interpretation of the results even more difficult than with other short-term tests. 

Reported toxicity values using the Vibrio fisheri and the Cellsense biosensor either with P. putida or E. coli expressed in 50% inhibition (EC50) and toxicity units (TUs) for some pollutants [60]... 

Castro, E.A., Toropov, A.A., Netserova, A.l. and Nazarov, A.U. (2003) QSAR study of the toxic action of aliphatic compounds to the bacteria Vibrio fisheri based on correlation weighting of local graph... 

Cronin, M.T.D. Schultz, T.W. 1997. Validation of Vibrio fisheri acute toxicity data mechanism of action-based QSARs for non-polar narcotics and polar narcotic phenols. Science of the Total Environment, Vol. 204, No 1, pp. 75-88. 

The test is based on measuring the luminescence of luminescent bacteria Vibrio fisheri and was developed for measuring the toxicity of solid and coloured samples [13]. 

The ecotoxicological impact of lactic acid-based polymers was evaluated by biotests, i.e. by the Flash test, measuring the inhibition of light production of Vibrio fisheri, and by plant growth tests with cress, radish, and barley [16]. Poly(lactic acid)s, poly(esterurethane)s... 

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