Very-high-throughput screening

In conclusion, PyMS is undoubtedly very useful for the discrimination of micro-organisms at the genus, species and subspecies level, and whilst it has relatively low throughput (2 min per sample), which would make it unsuitable for very-high-throughput screening programmes, it does present itself as a suitable method for the rapid, precise and accurate analysis of the biochemical composition of bioprocesses. 

Combinatorial catalyst research will help satisfy the demands placed on new process technologies. As 90% of all production processes work with catalysts to ensure efficient, cost-effective conversion, they have a key role to play. In future, combinatorial research will generate what was once mainly acquired empirically. With combinatorial methods, many possible design variants can be tested very reliably and within a short time, to locate their optimum behavior. The information gathered includes the type and structure of the required catalyst carrier as well as catalytic conversion rates. To achieve this, modern machines with a very high throughput/screening rate... 

The use of direct UV spectrophotometry to measure sample concentrations in pharmaceutical research is uncommon, presumably because of the prevalence and attractiveness of HPLC and LC/MS methods. Consequently, most researchers are unfamiliar with how useful direct UV can be. The UV method is much faster than the other methods, and this is very important in high-throughput screening. 

The best combination turned out to be L5 /A9 (ee = 90% at room temperature and 99% at —78 °C with benzaldehydes and ee = 92-99% with other aldehydes).87 Further improvements were reported later.89 Although only a few dozen reactions were monitored by a JASCO-CD-995 instrument, the CD-based assay is amenable to high-throughput screening of enantioselective catalysts. The chemistry itself lends itself ideally to combinatorial asymmetric metal catalysis, since the principle of asymmetric activation is turning out to be very powerful.89... 

However, many recent instruments are still not considered satisfactory, since professional developers in the field of high-throughput screening (HTS) want to use the full performance of the latest generation of robots and computers for automation. This results in new instrumental developments, like the possibility of reading not only 96, but 384 or even 1536 wells plates as well as DNA chips, very rapidly (in a minute or so) and repeatedly without any mechanical failures. Hence, in the eyes of company scientists developing new assays, many present-day instruments still correspond to an intermediate stage of development. For research laboratory scientists, on the other hand, the actual equipment offers excellent performance. 

Mass spectrometry is the most preferred technique employed during high-throughput screening. It provides specificity based on its capability to monitor selected mass ions, sensitivity because it affords enhanced signal-to-noise ratio, and speed due to very short analysis times that allow analysis... 

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