Vasopressin cell culture

Mirror-image DNA Inhibiting Vasopressin in Cell Culture... 

Goldberg H, dayman P, Skorecki K Mechanism of U inhibition on vasopressin-sensitive adenylate cyclase in cultured renal epithelial cells. Am J Physiol 24 F995-F1002, 1988... 

Williams, B., P. Tsai, and R.W. Schrier. 1992. Glucose-induced downregulation of angiotensin II and arginine vasopressin receptors in cultured rat aortic vascular smooth muscle cells. Role of protein kinase C. J. Clin. Invest. 90 1992-1999. 

Ausiello DA, Hall DH, and Dayer JM. Modulation of cyclic AMP-dependent protein kinase by vasopressin and calcitonin in cultured porcine renal LLC-PK1 cells. Biochem J 186 773-780,1980. 

Lang MA, Muller J, Preston AS, and HandlerJS. Complete response to vasopressin requires epithelial organization in A6 cells in culture. Am J Physiol 250 Cl 38-145,1986. 

Growth substrate characteristics influence epithelial cell orientation and phenotypic expression due to the interaction of receptor sites on the cell surface with specific sites in the substrate/matrix. For example, growth of the Xenopus laevis kidney cell hne A6 on microporous substrates could induce the expression of vasopressin receptors absent in solid support grown monolayers [157]. Similar observations have been reported when rabbit primary cultures of renal cortical collecting ducts were maintained on microporous supports under constant medium perfusion [158]. 

Other mediators have also been related to CsA-in-duced functional nephrotoxicity. Increased plasma level of adenosine due to reduced uptake by red blood cells was observed in CsA-treated renal transplant recipients [183]. In the same way, rats receiving CsA showed increased concentration of adenosine in renal artery paralleled by a decrease in mRNA expression for Aj and A2a renal adenosine receptors [184]. Experimental use of selective Aj adenosine receptors antagonists or theophylline resulted in contradictory results with some authors finding renal hemodynamic and functional protection whereas other did not [38, 185-187]. Moderate increases in plasma vasopressin were observed in CsA-treated renal transplant recipients [188] and CsA enhanced vasopressin-induced rise in intracellular calcium in cultured glomerular mesangial... 

Angiotensin II, vasopressin, and endothelin inhibit K tp channels in cultured coronary artery smooth muscle cells (e.g., Miyoshi et al, 1992). Muscarinic receptor stimulation inhibited K jp channels in smooth muscle cells in urinary bladder through stimulation of protein kinase C (Bonev and Nelson, 1993b). Serotonin, phenylephrine, histamine, and neuropeptide Y also inhibit K jp currents in smooth muscle cells from mesenteric arteries through stimulation of protein kinase C (Bonev and Nelson, 1995). 

There are a few cases of alkaloids that act at the receptors for peptide hormones. Spiroquinazoline inhibits the binding of substance P to its receptor on astrocytoma cells [315]. Chelerythrine and sanguinarine compete with vasopressin for receptors on rat liver cells [316]. Psycholeine is an antogonist at somatostatin receptors on cultured pituitary cells [317, 318]. Caffeine inhibits the binding of thyrotropinreleasing hormone to pituitary cells [319]. 

A number of studies have investigated the effects of peripheral neurotransmitters and peptides on the regulation of NGF production and secretion by isolated organs or cells. For example, Hellweg et al. (1988) examined the influence of many agents on the release of NGF into the medium of cultured irides. Addition of neuropeptide Y, substance P, vasopressin, somatostatin, vasoactive intestinal polypeptide, neurotensin, serotonin and histamine all failed to change NGF levels. However, as these experiments were carried out in serum that may have resulted in maximal stimulation of the NGF gene, only inhibitory effects may have been detectable. Indeed, noradrenaline and dopamine were shown to be inhibitory. 

Activation ofV receptors by desmopressin or vasopressin increases circulating levels of procoagulant factor VIII and von Willebrand factor These effects are mediated by extrarenal V receptors. Presumably, vasopressin stimulates the secretion of von Willebrand factor and of factor VIII from storage sites in vascular endothelium. Since release of von Willebrand factor does not occur when desmopressin is applied directly to cultured endothelial cells or to isolated blood vessels, intermediate factors are likely to be involved. 

Turk MB, Thompson MM, Corjay MH, Owens GK (1991) Mechanisms of angiotensin II- and arginine vasopressin-induced increases in protein synthesis and content in cultured rat aortic smooth muscle cells. Circ Res 68 288-299... 

Beck, T.R., Hassid, A. and Dunn, M.J. (1980). Effect of arginine vasopressin and its analogues on the synthesis of PGE2 by rat renal medullary interstitial cells in culture. J. Pharmacol Exp. Ther., 215, 15-19... 

Zusman, R.M. and Keiser, H.R. (1977). Prostaglandin biosynthesis by rabbit renomedullary interstitial cells in tissue culture. Stimulation by angiotensin II, bradykinin and arginine vasopressin. J. Clin. Invest., 60, 215—23... 

Pugliese, F., Sato, M., Williams, S., Aikawa, M., Hassid, A. and Dunn, M. (1983). Rabbit and rat renal papillary collecting tubule cells in culture The interactions of arginine vasopressin, prostaglandins and cyclic AMP. In Samuelsson, B., Ramwell, P.W. and Paoletti, R. (eds.) Advances in Prostaglandin, Thromboxane and Leukotriene Research, pp.517-23. (New York Raven Press)... 

Goldring, S.R., Sayer, J.M., Ausiello, D.A. and Krane, S.M. (1978). A cell strain cultured from porcine kidney increases cyclic AMP content upon exposure to calcitonin or vasopressin. Biochem. Biophys. Res. Commun., 83, 434-40... 

Arg ]-vasopressin stimulated surfactant secretion in primary cultures of rat type 2 pneumocytes independently of adenosine 3 ,5 -cyclic monophosphate (Brown and Wood 1989). A 50% loss of tritiated phosphatidyhnsositol 4,5-biphosphate (PIP2) occurred from cells prelabeled with myo[ H]inositol within 15 s (Brown and Chen 1990). Consistent with vasopressin-induced PIP2 hydrolysis the two breakdown products, 1,2-diacylglycerol and inositol 1,4,5-triphosphate, was observed. Vasopressin stimulated protein kinase C activity twofold over the basal activity of 0.7410.07 nM/min x mg protein. The [Arg ]-vasopressin antagonist, 1-deamino-8-D-arginine vasopressin, inhibited [Arg ]-vasopressin activation of protein kinase C. 

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