Antibody, variable fragment

Fu Y, Shearing LN, Haynes S, Crewther P, Tilley L, Anders RF, Foley M, Isolation from phage display libraries of single chain variable fragment antibodies that recognize conformational epitopes in the malaria vaccine candidate, apical membrane antigen-1, J. Biol. Chem., 272(41) 25678-25684, 1997. 

Deng X K, Nesbit L A, Morrow K J, Jr (2003). Recombinant single-chain variable fragment antibodies directed against Clostridium difficile toxin B produced by use of an optimized phage display system. Clin. Diagn. Lab. Immunol. 10 587-595. 

Choi G H, Lee D H, Min W K, et al. (2004). Cloning, expression, and characterization of single-chain variable fragment antibody against mycotoxin deoxynivalenol in recombinant Escherichia coli. Protein Expr. Purif. 35 84-92. 

Variable Fragment (Fv) The tips of the two Y arms vary greatly from one antibody to another. They are the regions that bind to epitopes of antigens and bring them to the natural killer cells and macrophages for destruction. 

De Wildt, R.M.T., Finnem, R., Ouwehand, W.H., Griffiths, A.D., Van Venrooy, W.J., and Hoet, R.M.A. (1996). Characterization of human variable domain antibody fragments against the U1 RNA-associated A protein, selected from a synthetic and a patient derived combinatorial V gene library. Eur. J. Immunol., 26, 629-639. 

Haidaris, C. G., Malone, J., Sherrill, L. A., Bliss, J. M., Gaspari, A. A., Insel, R. A., et al. (2001) Recombinant human antibody single chain variable fragments reactive with Candida albicans surface antigens. J. Immunol. Meth. 257, 185-202. 

Figure 32.3. The structure of the antibody molecule. All immunoglobulins consist of a basic subunit made up of four polypeptide chains (two light and two heavy) bound together by disulfide bonds. The variable region of the molecule contains the antigen binding site. Papain digestion yields a constant fragment (Fc) and variable fragments (Fab).

RPPs have been designed to capture proteins from cellular or tissue lysate samples or present in the serum or plasma onto surfaces (12), which are further identified by an affinity reagent such as antibody, single-chain variable fragments, and aptamers, among others. Thus, the signal could be related to specific protein detection and it could be affected by antibody affinity and... 

S. Petit, F. Brard, G. Coquerel, G. Perez, F. Tron. Structural models of antibody variable fragments a method for investigating binding mechanisms. J Comput Aided Mol Des. 1998, 12, 147-163. 

SJ Deng, CR MacKenzie, J Sadowska, et al. Selection of antibody single-chain variable fragments with improved carbohydrate binding by phage display. J Biol Chem 269 9533-9538, 1994. 

Rippmann J F, Klein M, Floischen C, Brocks B, Rettig W J, Gumpert J, Pfizenmaier K, Mattes R, Moosmayer D (1998). Procaryotic expression of single-chain variable-fragment (scFv) antibodies Secretion in L-form cells of Proteus mirabilis leads to active product and overcomes the limitations of periplasmic expression in Escherichia coli. Appl. Environ. Microbiol. 64 4862-4869. 

Olea-Popelka F, McLean M D, Horsman J, et al. (2005). Increasing expression of an anti-picloram single-chain variable fragment (ScFv) antibody and resistance to piclo-ram in transgenic tobacco (Nicotiana tabacum). J. Agric. Food Chem. 53 6683-6690. 

Sapats S I, Heine H G, Trinidad L, et al. (2003). Generation of chicken single chain antibody variable fragments (scFv) that differentiate and neutralize infectious bursal disease virus (IBDV). Arch. Virol. 148 497-515. 

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