Vacuum degas

Adhesive flash is excessively Excess air stirred into Vacuum-degas adhesive before... 

Typical furnace camera coverage of walking beam furnace in steel industry. ("High-Temperature Furnace Camera Systems/ Lenox Application Solutions in the Steel Industry (reference sheets with diagrams for reheat furnaces, vacuum degas-sers, remelt/reverberatory furnaces).)... 

Adhesive flash is excessively porous Excess air stirred into adhesive Solvent not completely dried out before bonding Adhesive material contains volatile constituent Vacuum-degas adhesive before application Increase dr3ring time or temperature Seek advice from manufacturers... 

Suspend 300 mg of agarose and 100 mg of gelatin in 20 ml- of overlay buffer in a small suction flask. Heat mixture in a microwave oven until the agarose is dissolved. Vacuum degas solution briefly. Pour solution into preheated glass plate assembly. 

Vacuum degas or decontaminate titanium beta alloys that absorb hydrogen in reducing baths... 

Vacuum-degas adhesive before application. Increase drying time or temperature. Make sure drying area is properly ventilated... 

The dilatometer is generally made of glass and is the vessel where the mercury is intruded into the sample pores. The design is dependent on the pressure source and monitoring system of the instrument. The dilatometer consists of a sample holder and a calibrated stem, which is used to measure the amount of mercury intruded into the sample. The sample in the dilatometer must be cleaned from adsorbed species by degassing the material in a vacuum [42], Most commercial instruments degas the sample in the instrument before mercury intrusion. Once the sample is degassed, the dilatometer (sample holder and stem) are filled with mercury. 

A sample of monomer ( 50 g) was placed in a 100-ml round bottomed flask, which was attached to a rotary evaporator. The flask was heated to 150°C with the aid of an oil bath while stirring under vacuum (0.20 mmHg). The monomer was stirred and heated under vacuum for a period of 45 min to 1 h in order to simultaneously degas and B-stage the monomer. The B-staged monomer was then held under nitrogen and poured into a preheated mold, which consisted of two polished aluminum plates (6 x 6 x 1/2 in.) with a three-sided 1/8-in. thick Teflon spacer between the two plates. The spacer was cut from a 6 x 6 x 1/8 in. 

Mobile phase compositions for this experiment are polar methanol-water mixtures in the ratios 90/10, 80/20, and 70/30 by volume. The stationary phase is C18. Prepare 200 mL of each mobile phase and then filter and degas each through 0.45-/./m filters with the aid of a vacuum (instructor will demonstrate). Slowly pour each (so as to avoid reaeration by splashing) into individual mobile phase reservoirs that are labeled appropriately. 

Prepare 500 mL of a mobile phase that is 25 mM KC1, 5 mM MgCl2, and 50 mM tris-(hydroxym-ethyljamino methane (TRIS or THAM). Adjust the pH to 7.2 using concentrated HC1. Filter and degas this mobile phase using a vacuum filtration apparatus equipped with 0.45-/LL filters. 

Figure 6.3. Levitation of a molten metal in a radio-frequency field. The coil consists of water-cooled copper tubes. The counter winding above the sample stabilizes levitation. The same coils (and possibly additional ones) act as the induction heater. This technique has been applied to container-less melting and zone refining of metals and for drop calorimetry of liquid metals. It can be also used to decarburize and degas in ultrahigh vacuum mono-crystalline spheres of highly refractory metals (adapted from Brandt (1989)). The arrows indicate the instantaneous current flow directions in the inductors.

Vacuum source or a bath sonicator to degas buffers. 

Filter BBS through a 0.45-)im filtration membrane. Degas the buffer by applying vacuum for 30 min or by sonicating in a bath sonicator for 5 min. 

Degas the matrix under vacuum and pack the HR 5/10 column with the washed matrix. Equilibrate the column with 40 mL 100 mM sodium phosphate and check for proper column packing. The matrix should be free of particles, air bubbles, or cracks. Apply 20 mL of filtered 100 mM glycine, pH 3.0, followed by 20 mL of 100 mM sodium phosphate, pH 8.0 (see Note 2). 

Lilter all buffers through a 0.45- am filter and degas the solutions under vacuum or by sonication. 

Slurry the swollen, settled gel in about a half of its volume of buffer and degas under vacuum for 10 min. Connect the column outlet with tubing ending in a vessel at the level of the upper border of the column. Then mount the filling reservoir to the vertical column as shown in Fig. 3.3A. Fill the column up to one-fourth to one-third of its height with buffer and pour the gel slurry without bubbles in one portion into column and reservoir. Regulate the recommended hydrostatic pressure Ah (cf. Fig. 3.3 Table 3.2) by lowering the outlet. 

Note Values refer to the amount remaining from a 5-mL sample. Solutions were prepared on a generator column (13). b Sample was exposed to vacuum for 60 s to degas. 

Nitrogen adsorption measurements were done using a Micromeritics model ASAP 2010 adsorption analyzer (Norcross, GA). Adsorption isotherms were measured at -196°C over the interval of relative pressures from 10 6 to 0.995 using nitrogen of 99.998% purity. Before each analysis the sample was degassed for 2 hours at 150°C under vacuum of about 10 Torr in the degas port of the adsorption apparatus. 

Degas the slurry using a Buchner side arm flask under vacuum for 1-2 h with periodic swirling. Do not use a magnetic stirrer, since this may damage the beads. 

G25 column buffer (AE, 0.05A/) 3 35 g sodium acetate, 0 526 mL glacial acetic acid, 0 186 g EDTA, made up to 1 L. Degas before use, e.g, under vacuum (see Chapter 10) or by using nitrogen... 

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