Umbilical cord blood, hematopoietic stem cells

Gluckman E. (2000) Current status of umbilical cord blood hematopoietic stem cell transplantation, Exp HeinatolZS, 1197-1205. 

Chua KN et al (2006) Surface-aminated electrospun nanofibers enhance adhesion and expansion of human umbilical cord blood hematopoietic stem/progenitor cells. Biomaterials 27(36) 6043-6051... 

Chua, K. N., C. Chai, P. C. Lee et al. 2007. Functional nanofiber scaffolds with different spacers modulate adhesion and expansion of cryopreserved umbilical cord blood hematopoietic stem/progenitor cells. Exp Hematol 35(5) 771-81. 

Transplant with umbilical cord blood (UCB) offers an alternative stem cell source to patients who do not have an acceptable matched related or unrelated donor. When allogeneic hematopoietic cells are obtained from UCB, the cord blood is obtained from a consenting donor in the delivery room after birth and delivery of the placenta.32 The cord blood then is processed, a sample is sent for HLA typing, and the cord blood... 

The bone marrow is not the only source of hematopoietic stem cells. Such cells are present in low numbers in peripheral blood, and these can be expanded in number with specific factors. Peripheral blood stem cells have the major therapeutic advantage that their harvest is relatively painless and less invasive. Likewise, umbilical cord stem cells can also be obtained in a non-invasive manner from... 

Stem cell therapy involves infusion of specialized cells utilized to perform specific functions. The traditional use of cell therapy includes harvest and cryopreservation of autologous hematopoietic cells either from the bone marrow (old approach) or mobilization and pheresis of hematopoietic stem cells from peripheral blood using stem cell-mobilizing cytokines such as hematopoietic colony-stimulating factors (G-CSF, GM-CSF) or chemokine inhibitors (AMD-3100). A more recent stem cell source is umbilical cord blood that has rich pleuripotent potential and can engraft at lower doses than bone marrow or mobilized peripheral blood stem cells. 

Mayani H, Lansdorp PM, 1998. Biology of human umbilical cord blood-derived hematopoietic stem/progenitor cells. Stem Gells 16 153... 

Broxmeyer, H.E., Gordon, G.W., Hangoc, G. et al. (1989) Human umbilical cord blood as a potential source of transplantable hematopoietic stem/ progenitor cells, Proc. Natl. Acad. ScL t/S 86 3828-3832. 

There are three sources of hematopoietic stem and progenitor cells available bone marrow (BM), peripheral blood after stem cell mobifization (MPB) and umbilical cord blood (CB). 

Many patients do not have HLA-matched family members or unrelated donors in the marrow registries. An alternative for these patients is the use of human umbilical cord blood, which contains hematopoietic stem cells capable of reconstituting bone marrow function following high-dose chemotherapy. " An almost unlimited number of cord blood donors is potentially available, because the cord and its associated blood are commonly discarded following delivery. 

Hematopoietic stem cells are found in the bone marrow, peripheral blood, and umbilical cord blood. Because of the rarity and similarity to other cells, hematopoietic stem cells are difficult to isolate and measure. These stem cells express the CD34 antigen, and the number of CD34+ cells has become a clinically useful measure of the number of hematopoietic stem cells. 

Hematopoietic stem cell transplantation (HSCT) is a process that involves intravenous infusion of hematopoietic stem cells from a compatihle donor into a recipient, usually following administration of high-dose chemotherapy. Hematopoietic stem cells can be derived from the bone marrow, peripheral blood, or umbilical cord blood. The rationale for HSCT in the treatment of malignant disease is based on studies that show that most anticancer drugs have a steep... 

Chen N, Hudson J E, Walczak P, et al. (2005). Human umbilical cord blood progenitors The potential of these hematopoietic cells to become neural. Stem Cells. 23 1560-1570. 

Patterson J, Moore CH, Palser E, Hearn JC, Dumitru D, Harper HA, Rich IN. Detecting primitive hematopoietic stem cells in total nucleated and mononuclear cell fractions from umbilical cord blood segments and units. J Transl Med. 2015 13 94. 

Regarding the source of hematopoietic stem cells, the transplant maybe from bone marrow, mobilized peripheral blood or umbilical cord blood. 

Fig. 9 Migration of hematopoietic stem cells isolated from umbilical cord blood on various collagen samples. Concentration was 1.2mg/ml for collagen and 0.4mg/ml for heparin and hyaluronic acid, respectively...

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