Ultra-performance liquid chromatography UPLC

Wren, S. A. Peak capacity in gradient ultra performance liquid chromatography (UPLC). / Pharm Biomed Anal 2005, 38, 337-343. 

Swartz, M. E. (2005a). Ultra performance liquid chromatography (UPLC) An introduction. LCGCN. Am. 8-14. 

Ultra-performance liquid chromatography (UPLC) analysis of free amino acids of "on-lees" aged wines gave time- and yeast-dependent amounts of histidine, ll.l-21.1mg/L, and proline 769.7-895.7mg/L (11JC(B)1361). 

Electrospray ionization (ESI), a very powerful MS ionization mode, has been coupled with liquid chromatography becoming a popular tool in biomolecular analysis and drug analysis applications [1], Use of HPLC or ultra performance liquid chromatography (UPLC) combined with a mass spectrometry through the interface—ESI has become a powerful and routinely used analytical tool for many fields in scientific research, pharma industry, healthcare, and clinical diagnostic applications. 

A relatively new analytical separation technique known as Ultra Performance HPLC or Ultra Performance Liquid Chromatography (UPLC ) is similar in principle to HPLC except that it uses smaller particle sizes (<2.5 pm) and higher flow rates to increase the speed of separation and peak capacity, or number of peaks resolved per unit time in gradient separations (Swartz, 2005). UPLC was used to separate and quantify six major polyphenolic compounds in cocoa with a 50 mm by 2.1 mm column with a 1.7 pm particles size and a run time of only... 

With respect to the HPLC methods applied, different liquid chromatographic method was developed by Jones et al. [15] to assess glimepiride and its degradation products. A Ultra Performance Liquid Chromatography (UPLC) method was utilized in conjunction with the MS detection. The use of UPLC can enhance the resolution as well as specificity of MS detection. In addition, shorter retention time is able to gained compared to HPLC [15,37]... 

Ultra performance liquid chromatography (UPLC) is an important advancement in chromatography due to the significantly improved speed, resolution, and sensitivity (160). UPLC typically uses separation media with a particle... 

Recently ultra-performance liquid chromatography (UPLC) has been introduced commercially as a new direction in liquid chromatography. In UPLC columns packed with 1.7-pm, particles are operated at pressures up to lOOMPa. The main advantage is obviously the reduction in analysis time that has been demonstrated for pharmaceutical analysis [16] and metabolomics [17]. 

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