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UHPLC Applications

Scientific, Darien, IL). Five compounds—ascorbic acid (dead time marker), hydroquinone, resorcinol, catechol, and 4-methyl catechol—were eluted with a 10/90 (v/v) acetonitrile/water mobile phase containing 0.1 % TFA and were detected with amperometric detection (+1.0V versus Ag/AgCl). The chromatogram was obtained near the optimum linear velocity at a run pressure of 3000 bar. All compounds eluted in less than 8 minutes, with efficiencies ranging from a low of 244,000 plates for 4-methyl catechol to as high as 330,000 plates for hydroquinone. These correspond to about 570,000 and 770,000 plates/m, respectively—much higher than the 150,000 plates/m typically seen with conventional columns. [Pg.784]


An example that could be classified as a hybrid of traditional HPLC and UHPLC applications is the work carried out by Garcia-Villalba et al. [10], in which the characterization and quantification of extra-VOO phenolic compounds by an RRLC (rapid resolution liquid chromatography) method coupled to diode-array and TOE MS detection systems was developed. The RRLC method, transferred from... [Pg.229]

Because LC and MS have both evolved rapidly over the last ten years, the majority of recent mass spectrometers is compatible with fast chromatography, as demonstrated in Figure 4.1, which makes MS(/MS) the detector of choice for many UHPLC applications. In this chapter, the compatibility between fast-LC and MS... [Pg.96]

UHPLC column technology will continue to develop, such that in addition to RP-UHPLC, HILIC, and SEC applications, increasing UHPLC applications involving HIC and lEC may be developed. UHPLC is in position to play an important role in high-resolution and high-throughput characterization of protein therapeutics. [Pg.250]

Figure 9.3 shows an impurity separation under conventional pressures with a 5 /mi particle, 2.1 x 150 mm column, and the same separation performed via UPLC using a 2.1 x 50 mm column with 1.7 /im particles. The run time was improved by a factor of six, with overall resolution comparable to that of the original separation on the 5 /an column. The application of UHPLC technology to impurity profile analysis can exert a significant impact on laboratory productivity by achieving a... [Pg.254]

To minimize unacceptable interruptions in highly regulated work flows, the smooth transfer of legacy methods from conventional to fast LC methods (via geometric transfer or method redevelopment) is a critical issue for implementing fast LC for pharmaceutical applications. Method transfer from HPLC to UPLC is discussed in detail in the literature.52,53 Moreover, method transfer software that provides parameter conversion between UHPLC and conventional HPLC is available from instrument vendors. [Pg.261]

Different developed analytical method are discussed in this chapter related to the determination of illicit substances in blood (either whole blood, plasma, or serum), OF, urine, and hair. These methods take into consideration the particular chemical and physical composition of the matrix and applies each time a suitable pretreatment to remove interfering and matrix effect, to maximize recoveries and to achieve a suitable enrichment if necessary. For liquid matrices the applications of the most common techniques are considered from simple PPT to SPE and LLE the results of recent works from literature are reported and new trends as online SPE, pSPE, automated LLE (SLE) or MAE are examined. Several stationary phases have been shown to be suitable for determination of illicit drugs Cl8, pentafluorophenyl, strong cation-exchange, and HILIC columns. The trend toward fast chromatography is investigated, both UHPLC and HPLC with appropriate arrangements moreover, results obtained with different ion sources, ESI, A PCI, and APPI are compared. [Pg.390]

A much more sensitive technique than NMR is MS detection combined with various separation techniques (e.g., high-performance liquid chromatography, HPLC ultrahigh-performance liquid chromatography, UHPLC capillary electrophoresis, CE gas chromatography, GC) that allow previous separation of metabolites. The application of MS enables analysis of large number of metabolites based on their interactions with the separation system and, above all, on the basis of their molecular weights. [Pg.245]

Magiera, S., Baranowska, 1. Rapid method for determination of 22 selected drugs in human urine by UHPLC/MS/MS for clinical application. J. AOAC hit. 97, 1526-1537 (2014)... [Pg.279]

Fiechter, G., Sivec, G., Mayer, H. K. (2013). Application of UHPLC for the simultaneous analysis of free amino acids and biogenic amines in ripened add-curd cheeses. Journal of Chromatography B. http //dx.doi.Org/10.1016/j.jchromb.2012.12.006. [Pg.301]

In addition to these larger solid core particles, smaller sub-2 pm solid core particles with particle sizes of 1.7 and 1.3 pm are commercially available. However, their theoretical potential with respect to separation efficiency and speed of analysis, which certainly exists, cannot be used in practice. This is because no HPLC systems are available with the necessary small volumes (of both the capillaries and detector cells) and which also have the data acquisition rate required to detect and record the separations achieved on these columns without loss. Today s modern UHPLC systems are already quite well optimized, but their performance is by far not good enough for these applications the manufacturers face further challenges here. As with all materials, it is highly recommended to check the batch-to-batch reproducibility especially of solid core phases. [Pg.207]

The aim of this chapter is to present the state of the art on UHPLC-MS(/MS) analysis of pesticides in food. It includes a selection of the most relevant papers recently published regarding instrumental and column technology focusing on UHPLC analysis with sub-2 pm and novel porous shell particle-packed columns. Sample treatment procedures such as QuEChERS, MIPs, and online SPE will also be addressed. MS strategies for the analysis of pesticide residues as well as to guarantee confirmation and identification such as the use of HRMS or alternative confirmation strategies will be discussed with relevant application examples. [Pg.19]

An overview of several recent applications of UHPLC-MS(/MS) methods for the multi-residue analysis of pesticides in food products has been presented. In order to cope with the necessity of high throughput and fast analysis of pesticide residues in food, all aspects of analytical method development, that is, sample extraction and clean-up, chromatographic analysis, and quantitation and confirmation aspects, must be taken into account. [Pg.41]

This chapter will focus on the use of UHPLC techniques in food metabolomics. It includes a selection of the most relevant works recently published. A discussion regarding the use of different stationary phases, advantages, and drawbacks of the technique will be addressed. Moreover, some applications of UHPLC in food metabolomics will also be discussed. [Pg.53]

UHPLC has been used recently in food metabolomics. Table 3.1 summarizes reeent publications of UHPLC separation mode applied in this field. Examples of different applications such as food quality and authenticity and food component... [Pg.53]


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