Genome, yeast

EIW Mewes, K Albermann, M Blihr, D Erishman, A Gleissner, J Elam, K Eleumann, K Kleme, A Maierl, SG Oliver, E Pfeiffer, A Zollner. Overview of the yeast genome. Nature 387(6634 Suppl) 7-65, 1997. 

OLIVER, S.G., WINSON, M.K., KELL, D.B., BAGANZ, F., Systematic functional analysis of the yeast genome, Trends Biotechnol., 1998,16, 373-378. 

Yeast gene arrays, 26 490, 491 Yeast gene deletion libraries, 26 490-492 Yeast genome, 22 515, 26 446 comparison of, 26 450t Yeast leavening... 

Phosphoryl group transfer reactions add or remove phosphoryl groups to or from cellular metabolites and macromolecules, and play a major role in biochemistry. Phosphoryl transfer is the most common enzymatic function coded by the yeast genome and, in addition to its importance in intermediary metabolism (see Chapter 5), the reaction is catalysed by a large number of central regulatory enzymes that are often part of signalling cascades, such as protein kinases, protein phosphatases, ATPases and GTPases. 

YG-S98 yeast genome chip. In side-by-side experiments, the Febit device performed in concordance to the Affymetrix chip. 

In a related application of this approach, Ferea et al. (1999) examined variations in gene expression of progeny during adaptive evolution. The yeast genomes were monitored in evolving strains subjected to growth under... 

The Wodicka efal. (1997) paper also defined the performance of fhe Affymetrix chip. Semiquantitative measurement of the absolute abundance of mRNA species was possible. Flybridization of total yeast-genomic DNA to the chips revealed the mean hybridization signal across 6049 probe sets to vary by 25% coefficient of variance (CV). The use of gDNA serves to normalize because most genes are represented only once in the population. In fact, the majority (98%) of the intensities were found to cluster well within two standard deviations. Thus, the concentration of a given mRNA could be estimated af >95% probability to reside within twofold of its actual concentration. Measurement at widely different total gDNA concentrations did not appreciably affect this outcome. 

Eromont-Racine, M., J.C. Rain, and P. Legrain, Toward a functional analysis of the yeast genome through exhaustive two-hybrid screens. Nat Genet, 1997.16(3) 277-82. 

An example of this technique is illustrated in Figure 9-23, showing the dramatic results this technique can produce. Segments from each of the more than 6,000 genes in the completely sequenced yeast genome were separately amplified by PCR, and each segment was deposited in a defined pattern to create the illustrated microarray. In a sense, this array provides a snapshot of the entire yeast genome. 

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