Microorganisms transport

Monofilament sutures are considered to be a better choice than multifilament ones in closing contaminated wounds. Multifilament sutures elicit more tissue reactions which may lessen tissue ability to deal with wound infections. They also have a capillary effect which could transport microorganisms from one region of the wound to another. The reason that multifilament sutures generally elicit more tissue reactions than their monofilament counterparts is because inflammatory cells are able to penetrate into the interstitial space within a multifilament suture and invade each filament. Multifilament sutures also have a larger surface area in contact with tissues which should be expected to elicit more tissue reaction. 

Persistence of pesticides in the environment is controlled by retention, degradation, and transport processes and their interaction. Retention refers to the abihty of the soil to bind a pesticide, preventing its movement either within or outside of the soil matrix. Retention primarily refers to the sorption process, but also includes absorption into the soil matrix and soil organisms, both plants and microorganisms. In contrast to degradation that decreases the absolute amount of the pesticide in the environment, sorption processes do not affect the total amount of pesticide present in the soil but can decrease the amount available for transformation or transport. 

Some alcohols, eg, propylene glycol, not only lower water activity but also have an additional preservative effect caused by the way they interfere with the ceU membrane transport system of the contaminating microorganisms. Surfactants (qv) may show a similar effect. 

Waters While MIC-causing bacteria may arrive at the surface of their corrosion worksite by almost any transportation system, there is always water present to allow them to become ac tive and cause MIC to occur. There are plenty of examples of even superpure waters having sufficient microorganisms present to feed, divide, and multiply when even the smallest trace of a viable food-stuff is present (e.g., the so-called watei for injection in the pharmaceutical industiy has been the observed subject of extensive corrosion of pohshed stainless steel tanks, piping, and so on). 

All of the transport systems examined thus far are relatively large proteins. Several small molecule toxins produced by microorganisms facilitate ion transport across membranes. Due to their relative simplicity, these molecules, the lonophore antibiotics, represent paradigms of the mobile carrier and pore or charmel models for membrane transport. Mobile carriers are molecules that form complexes with particular ions and diffuse freely across a lipid membrane (Figure 10.38). Pores or channels, on the other hand, adopt a fixed orientation in a membrane, creating a hole that permits the transmembrane movement of ions. These pores or channels may be formed from monomeric or (more often) multimeric structures in the membrane. 

Aquaporins are central players in mammalian physiology, but are also important in microorganisms and plants. The number of AQPs in plants is quite high angiosperms species, for example, express approximately 35 different AQPs divided into four families on the basis of their sequence. Moreover, plant AQPs might be considered multifunctional channels for their different transport properties. 

Measures to improve the present situation are urgently needed. These measures could be optimized by considering the influence of weather conditions on survival and transport of the microorganisms of faecal origin. 

Endosulfan is released to the environment mainly as the result of its use as an insecticide. Significant contamination is limited to areas where endosulfan is manufactured, formulated, applied, or disposed of. The compound partitions to the atmosphere and to soils and sediments. Endosulfan can be transported over long distances in the atmosphere, but the compound is relatively immobile in soils. It is transformed by hydrolysis to the diol and by microorganisms to a number of different metabolites. It is bioconcentrated only to low levels and does not biomagnify in terrestrial or aquatic food chains. 

Microorganisms that escape phagocytosis in a local lesion may now be transported to the regional lymph nodes via the lymphatic vessels. If massive invasion occurs with which the resident macrophages are unable to cope, microorganisms may be transported through the thoracic duct into the bloodstream. The appearance of viable microorganisms in the bloodstream is termed bacteraemia and is indicative of an invasive infection and failure of the primary defences. 

Microbial cells transported with the stream of fluid above the surface interact with conditioning films. Immediately after attachment, microorganisms initiate production of slimy adhesive substances, predominantly exopolysaccharides (EPS) that assist the formation of microcolonies and microbial films. EPS create bridges for microbial cells to the substratum and permit negatively charged bacteria to adhere to both negatively and positively charged surfaces. EPS may also control interfacial chemistry at the mineral/biofilm interface. 

In this chapter, we shall focus on the molecular aspects of amino acid transport and its regulation in Saccharomyces cerevisiae. Kinetic, biochemical and genetic aspects of the amino acid transport systems of eukaryotic microorganisms have been reviewed earlier [7,8]. 

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