Single molecule microscopy

Croci M, Irngartinger T, Renn A and Wild U P 1994 Single molecule microscopy Exp. Tech. Phys. 41 249-57... 

Schmidt T 1998 Detection of individual oligonucleotide pairing by single-molecule microscopy Ana/. Chem. 71 279-83... 

Douglass AD, Vale RD. Single-molecule microscopy reveals 35. 

Douglass AD, Vale RD Single-molecule microscopy reveals plasma membrane microdomains created by protein-protein networks that exclude or trap signaling molecules in T cells. Cell... 

G. Jung, J. Wiehler, B. Steipe, C. Brauchle, A. Zumbusch, Single-molecule microscopy of the green fluorescent protein using two-color excitation. Chem. Phys. Chem. 2,392 (2001)... 

Figure 22. Sketch of the experimental setup of the fluorescence microscope with light source, cryostat, and detection system. Three insets show the different optical setups used, (a) The first singlemolecule microscope with the optics outside the cryostat (b) modified commercially available microscope objective immersed in liquid helium (c) mirror objective especially designed for low temperature single molecule microscopy. Adapted from Ref. 44.

Mortehnaier, M, Kogler, EJ, Hesse, J, Sonnleitner, M, Huber, LA, and Schutz, GJ, Single molecule microscopy in hving cells Subtraction of autofluorescence based on two color recording. Single Molecules 3 (2002) 225-231. 

Heinz, WF, Weston, KD, Jolivet, V, Navarro, B, Bernardi, P, and Goldner, LS, A combined atomic force, confocal, and total internal reflection microscope for single molecule microscopy. Biophysical Journal 82 (2002) 201. 

ECFP" 434/477 26,000 40 - No FCS reports - Tested less suitable for single-molecule microscopy - Low photostability Blue [32,107]... 

Certain chromophore systems are intrinsically predisposed for ultrafast single molecule microscopy. Among these, emitters coupled to metal surfaces stand out as exceptionally well-suited subjects. Numerous observations of substantial radiative rate enhancement at the surface or in the vicinity of the surface of a metal were reported. Radiative rate enhancements as large as 10 have been predicted for molecular fluorophores and for semiconductor quantum dots coupled to optimized nanoantennae.Such accelerated emission rates put these systems well within the reach of the emerging femtosecond microscopy techniques. As a result, we decided to apply the Kerr-gated microscope to study of fluorescence dynamics of individual core-shell quantum dots in contact with smooth and nanostructured metal surfaces. 

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