Tobacco alkaloids capillary

Lochmann, H., Bazzanella, A., Kropsch, S., and Bachmann, K. (2001). Determination of tobacco alkaloids in single plant cells by capillary electrophoresis.. Chromatogr. A 917, 311 — 317. 

Harke and Drews used a 50 m long stainless steel capillary column, 0.5 mm I.D., coated with Ucon LB 550 X (polyethylene glycol) and K0H for the separation of tobacco alkaloids. A typical chromatogram of a test-mixture containing 3-pyridyl-n-propylketon, nicotine, nor-nicotine, myosmine, anabasine and nicotyrine is shown in Figure 5.1 (Tobacco alkaloids). 

To identify tobacco alkaloids and their mammalian metabolites, Pilotti et al. made use of capillary gas chromatography-mass spectrometry using glass capillary columns ( 33 m by... 

Pilotti et al. carried out studies on the identification of tobacco alkaloids, their mammalian metabolites and related compounds by gas chromatography-mass spectrometry using packed columns (SE-30, SE-52 and Carbowax 20 M + KOH) and capillary columns (33 m - Emulphor 0 and 9.6 m - 0V-101). Various pyridine compounds, either identified or implied as intermediates in the manmalian metabolism of nicotine present in tobacco or tobacco smoke, were studied by GC-MS. Preliminary GC-MS experiments on the determination of nicotine using capillary columns in combination with multiple ion detection (MID) employing deuterated nicotine as internal standard were reported. The gas chromatographic data of the compounds investigated... 

Thin-layer chromatography is frequently used to separate pyridine alkaloids from tobacco extracts. This method is frequently used by tobacco breeders to screen plant populations and individual tobacco plants to insure seed purity. Thin-layer is considerably faster than paper chromatography and can also be used as a preliminary cleanup for more sensitive methods for analytical analysis such as capillary gas chromatography (GC). Semi-... 

Pyridine alkaloids in tobacco are usually determined by gas chromatography. The parent alkaloids - nicotine, nornicotine, anatabine, anabasine, and myosmine - may be determined in a single extract. A capillary GC procedure we use (Andersen et al. 1990) is based on that of Severson et al. (1981) but with modification. 

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